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In the present study, the histological effect of titanium dioxide nanoparticles (TDN) on testicular tissue of mature Mus musculus mice was investigated. The animals were divided into six groups, control group treated with TDN free saline and five groups treated with TDN as follow 5, 10, 50, 100 and 150 mg/kg B.W. The results showed that TDN has histological effects on testicular tissue like sever congestion, mild edema between seminiferous tubules (STs) and decrease the thickness of germinal epithelium at low concentrations, While, the histological changes at high concentrations involved disturbance in STs diameters, sever edema between STs, sever vaculation in the germinal epithelium and necrosis in spermatogonia, germinal epithelium and Sertoli cells.
Ahotupa M, and Huhtaniemi I. (1992). Impaired detoxification of reactive oxygen and consequent oxidative stress in experimentally criptorchid rat testis. Biol. Reprod., 46: 1114-1118.
Ashraf MM, Marwa IA, and Peter AN (2015) Reproductive toxicity investigation of titanium dioxide nanoparticles in male albino rats. W.J. Pharm. and Pharmaceut. Sci. 10 (4): 34-49.
Coskun O, Ocakci A, Bayraktaroglu T, and Kanter M (2004). Exercise training prevents and protects streptozotocin-induced oxidative stress and beta-cell damage in rat pancreas. Tohoku J. Exp. Med. 203. 145-154.
Culling C.F.A.; Allison R.J. and Barr W.T. (1985). Cellular pathology technique. Butterworth and Co. (Publishers) 1st ed., London.
Dankovic D, Kuempel E, and Wheeler M (2007) An approach to risk assessment for TiO2. Inhal Toxicol, 19(Suppl 1):205–212.
Ferrini M, Wang C, Swerdloff R, SinhaHikim A, Rajfer J, and Gonzalez-Cadarid N. (2001). Aging related increased expression of inducible nitric oxide synthase and cytotoxicity markers in rat hypothalamic region associated with male reproductive function. Neuroendocrinology, 74 (1): 1-11.
Fujishima A, Zhang X, and Tryk DA. (2008). Ti photocatalysis and related surface phenomena. Surface Science Reports, 63: 515-582.
Gao G, Ze Y, Zhao X, Sang X, Zheng L, Ze X, Gui S, Sheng L, Sun Q, Hong J, Yu X, Wang L, Hong F, and Zhang X. (2013). Titanium dioxide nanoparticle-induced testicular damage, spermatogenesis suppression, and gene expression alterations in male mice. Journal of Hazardous Materials, 259:133-143.
Herrero MB, Viggiano JM, Martinez SP, and Gimeno MF, (1997). Evidence that nitric oxide synthase is progesterone-induced acrosomal exocytosis in mouse spermatozoa. Repro, Fert. and Devel., 9:1 -10.
Hongbo S, Ruth M, Vincent C and Jinshun Z (2013) Titanium dioxide nanoparticles: a review of current toxicological data. Particle and Fibre Toxicology, 8977-10-15.
Jiang J, Oberdorster G, Elder E, Gelein R, Mercer P, and Biswas P. (2008). Does nanoparticle activity depend upon size and crystal phase? Neurotoxicology, 2: 33-42.
Komatsu T, Tabata M, Kubo-Irie M, Shimizu T, Suzuki K, Nihei Y, and Takeda K (2008) The effects of nanoparticles on mouse testis Leydig cells in vitro. Toxicol In Vitro, 22:1825–1831.
Liu H, Ma L, Zhao J, Liu J, Yan J, Ruan J, and Hong F (2009) Biochemical toxicity of nanoanatase TiO2 particles in mice. Biol Trace Elem Res, 129:170–180.
Ma L, Liu J, Li N, Wang J, Duan Y, Yan J, Liu H, Wang H, and Hong F (2010). Oxidative stress in the brain of mice caused by translocated nanoparticulate TiO2 delivered to the abdominal cavity. Biomaterials, 31:99–105.
Mahrousa MHK. (2004). Cytogenetic and biochemical effects of some food colors in rats. Ph. D. thesis submitted to Animal Production Department, Faculty of Agriculture, Cairo University.
Medina C, Santos-Martinez MJ, and Radomski A. (2007). Nanoparticles: Pharmacological andtoxicological significance. Br. J. Pharmacol., 150: 552-558.
Nabela I, EL- Sharkawy S, Hamza M, and Ehsan, HA, (2010). Toxic Impact of Titanium Dioxide (TiO) In Male Albino Rats with Special Reference to its Effect on Reproductive System. J Ameri.Sci. 6(11): 865-872.
Schkroeder HA, Balassa JJ, and Tipton IH (1963) Abnormal trace metals in man: titanium. J Chronic Dis, 16:55–69.
Trouiller B, Reliene R, Westbrook A, Solaimani P, and Schiestl RH. (2009). Titanium dioxide nanoparticles induce DNA damage and genetic instability in vivo in mice. Cancer Res. 69: 8784-8789.
Wang J, Chen C, and Liu Y. (2008). Potential neurological lesion after nasal instillation of TiO nanoparticles in the anatase and rutile crystal phases. Toxicology Letters, (183): 72-80.
Wang J, Zhou G, Chen C, Yu H, Wang T, Ma Y, Jia G, Gao Y, Li B, Sun J, Li Y, Jiao F, Zhao Y, and Chai Z. (2007). Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration. Toxicology Letters, 168: 176-185.
Zhao J, and Castranova V (2011) Toxicology of nanomaterials used in nanomedicine. J Toxicol Environ Health B Crit Rev, 14:593–632.