Assessment of Bioplastic Producing Potential of Bacillus subtilis using Some Agricultural Residues as Carbon Source

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Abdullahi Balarabe Sallau Bashir Salim Aliyu Salihu


This study was carried out to assess the bioplastic producing potential of Bacillus subtilis using a variety of pretreated agricultural residues. Four agricultural residues (rice husks, molasses, bagasse, and corn cobs) were subjected to acid, alkaline and oxidative pretreatments using standard procedures. Polyhydroxybutyrate (PHB) produced was extracted using chloroform precipitation and quantified spectrophotometrically. The PHB production (g/L) for acid, alkali and hydrogen peroxide pretreatments were 1.52±0.02, 1.82±0.01, and 1.70±0.01 for rice husk;  1.82±0.01, 1.52±0.02, and 1.69±0.01 for molasses; 0.87±0.06, 1.10±0.10, and 0.96±0.07 for sugarcane bagasses and 0.5±0.00, 0.77±0.06, and 0.60±0.10 for corn cob, respectively. The maximum bioplastic yield of 63.94±2.59% was obtained in alkali pretreated corn cobs, while the lowest yield of 50.33±0.76% was found in acid pretreated rice husk. Thus, the findings in this study revealed that agricultural residues could be explored for PHB production in the presence of potential microbial strains due to their abundance, high carbon content, limited inhibitory effect and cost effectiveness.

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SALLAU, Abdullahi Balarabe; SALIM, Bashir; SALIHU, Aliyu. Assessment of Bioplastic Producing Potential of Bacillus subtilis using Some Agricultural Residues as Carbon Source. Science Journal of University of Zakho, [S.l.], v. 6, n. 2, june 2018. ISSN 2410-7549. Available at: <>. Date accessed: 22 aug. 2018. doi:
Science Journal of University of Zakho


Balat M., Balat H. and Oz C. (2008). Progress in bioethanol processing. Prog Energ Combust 34, 551 573.
Barham P.J. (1990). Physical properties of poly(hydroxybutyrate) and poly(hydroxybutyrate-co hydroxyvalerate). Novel Biodegradable Microbial Polymers, Kluwer, Dordrecht
Braunegg G., Lefebvre G. and Genser K.F. (1998). Polyhydroxyalkanoates, Biopolyesters from Renewable Resources: Physiological and Engineering Aspects. J Biotechnol. 65, 127 161
Byrom D. (1987). Polymer synthesis by microorganisms: technology and economics, TIBTECH 5, 156-167.
Castro-Sowinski S, Burdman S, Matan O, Okon Y (2009) Natural functions of bacterial polyhydroxyalkanoates. Microbiol Monogr. doi: 10.1007/978-3-642-03287- 5_3
Choi J. and Lee S Y (1997) Process analysis and economic evaluation for Poly (3-hydroxybutyrate) production by fermentation. Bioprocess Engineering, 17(6), 335-342
Dashtban M., Schraft H. and Qin W. (2009). Fungal bioconversion of lignocellulosic residues; opportunities and perspectives. Int Biol Sci, 5(6), 578–595
Gazaerly M.A. (1983). The utilization of beet molasses for single cell protein production [M Sc Thesis]. Botany Department, Faculty of Science, Alexandria University, Egypt.
Hassan, M. A., Shirai, Y., Kusubayashi, N., Abdulkarim, M. I., Nakanishi, K. and Hashimoto, K. (1996). Effect of Organic Acid Profiles during Anaerobic Treatment of Palm Oil Mill Effluent on the Production of Polyhydroxyalkanoates by Rhodobacter sphaeroides. Journal of Fermentation and Bioengineering 82(2), 151 – 156
Helal G.A. (1986). Protein and fats from halophilic fungi [Ph.D Thesis]. Department of Botany, Faculty of Science, Zagazig University, Egypt.
Hon DNS, Shiraishi N (2001) Wood and cellulosic chemistry, Second Edition. Dekker, New York
Jiun-Yee C., Sugama-Salim Y., Nyok-Sean L., Siew-Chen L., Raed M. M. A., and Kumar S. (2010) Bacterially Produced Polyhydroxyalkanoate (PHA): Converting Renewable Resources into Bioplastics (A. Mendez-Vilas: Editor). Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology.
Insomphun C., Kobayashi S., Fujiki T., and Numata K. (2016). Biosynthesis of polyhydroxyalkanoates containing hydroxyl group from glycolate in Escherichia coli. AMB Express, 6(29), 2016
Lopez J.A., Naranjo J.M., Higuita J.C., Cubitto M.A., Cardona C.A., Villar M.A. (2012). Biosynthesis of PHB from a New Isolated Bacillus megaterium strain : Outlook on Future Development with Endospore Forming Bacteria. Biotechnology and Bioprocess Engineering. 258, 250-258.
Rodriguea-Contreras A., Koller M, Miranda-de Sousa Dias M, Calafell-Monfort M, Braunegg G, Marqués-Calvo MS.(2013) High production of poly(3-hydroxybutyrate) from a wild Bacillus megaterium Bolivian Strain. Journal of Applied Microbiology, 114(5),1378-87.
Sanchez C., (2009). Lignocellulosic residues: biodegradation and bioconversion by fungi. Biotechnol Adv, 27(2), 185–194
Singh P., Ashwini P., Avinash A.K., Badri R., and Kajal D., (2009). Mutagenesis of Bacillus thuringiensis IA 12077 for increasing pol (-β-) hydroxybutyrate (PHB) production, Turk. J. Biol., 33, 225-230
Singh A, and Bishnoi N.R., (2013). Comparative study of various pretreatment techniques for ethanol production from water hyacinth. Ind Crops Prod, 44, 283–289
Singh P. and Parmar, N., (2011). Isolation and characterization of two novel polyhydroxybutyrate (PHB) producing bacteria. African J. Biotechnology, 10, 4902-919.
Steinbüchel, A. (2001). A. Perspectives for biotechnological production and utilization of biopolymers: metabolic engineering of polyhydroxyalkanoate biosynthesis pathways as successful example. Macro Biosci, 11:1-24
Sudesh K, and Iwata T (2008) Sustainability of biobased and biodegradable plastics. Clean – Soil, Air, Water 36: 433–442.
Tripathi, A.K., P.B. Tiwari, Mahima and D. Singh (2009) Assessment of air pollution tolerance index of some trees in Moradabad city. Indian J. Environ. Biol, 30:545-550.
Wang, H.H., Li, X.T., and Chen, G. Q. (2009). Production and characterization of homopolymer polyhydroxyheptanoate by a fadBA knockout mutant Pseudomonas putida KTOY06 derived from P. putida KT2442. Process Biochemistry 44(1), 106-111
Williams, S. F. and Martin, D. P. (2001) Applications of PHAs in Medicine and Pharmacy. Wiley -VCH, Weinham