Identification of P53 Tumor Suppressor Gene Alterations in Human Glioma Using Sscp-Pcr Technique.
Keywords:
Brain Tumors, Glioma, SSCP-PCR, P53Abstract
The P53 is the most commonly altered gene in cancers, which is located on chromosome 17p. Because of it is important role in the cell cycle it considered as the guardian of the genome that maintains genome integrity. In this study we analyzed DNA from 15 surgical specimens of human glioma for mutations in the p53 gene along exons 6-9 using single-strand conformation polymorphism analysis of polymerase chain reaction product (PCR-SSCP). The frequency of P53 gene mutation in gliomas examined was 26.6% (4 of 15). All 4 mutated cases were in exon 6 and no mutation was observed in exon 7,8 and 9. The results suggest the involvement of P53 gene mutation in Glioma and it might play an important role in the tumorigenesis process of patients with brain tumor in preliminary study in Duhok province.
References
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Kovach J S, McGovern R M, Cassady J D, Swanson S K, Wold L S, Vogelstein B and Sommer S S. (1991). Direct sequencing from touch preparations of Human carcinomas: Analysis of p53 mutations in Breast carcinoma; J. Natl. Cancer Inst. 83: 1004-1009.
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Quan, A. L.; Barnett, G. H.; Lee, S. Y.; Vogelbaum, M. A.; Toms, S. A.; Staugaitis, S. M.; Prayson, R. A.; Peereboom, D. M.; Stevens, G. H. J.; Cohen, B. H. and Suh, J. H.(2005). Epidermal growth factor receptor amplification does not have prognostic significance in patients with glioblastoma multiforme.
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Zupanska, A., and Kaminska, B. (2002). The diversity of p53 mutations among human brain tumors and their functional consequences. Neurochemistry International, 40, 637–645.
Barlow, A., and Ironside, J. W. (2009). Pathology of tumors of the central nervous system. Surgery, 27(3), 99-106.
Bartlett, J. M. S. and Stirling, D. (2003) .Methods in Molecular Biology :PCR Protocols (2nd ed). Humana Press Inc., New Jersey.
Bass, F., Bikker, H., Ommen, G.J., and Vijlder, J. (1984). Unusual scarcity of restriction site polymorphisms in human thyroglobin gene: A linkage study suggesting autosomal dominance of defective thyroglobin allele. Human genetics. 67, 301-305.
Chosdol, K.; Chattopadhyay , P. and Sinha ,S.(2002). Molecular pathways of glial tumorigenesis. Current science, 82(6): 648-654.
Cui W, Kong X, Cao HL, Wang X, Gao JF, Wu RL, Wang XC.( 2008) Mutations of p53 gene in 41 cases of human brain gliomas, Ai Zheng ,27(1):8-11.
Fomchenko, E. I. and Holland, E. C. (2005). Stem cells and brain cancer. Experimental cell Research, 306, 323– 329.
Gomes , A. L.; Reis-Filho ,J. S.; Lopes , J. M. ; Martinho, O. ; Lambros ,M. B.K. ; Martins ,A.; Schmitt F.; Pardal ,F. and Reis, R. M. (2007). Molecular alterations of KIT oncogene in gliomas. Cellular Oncology, 29: 399–408.
Haberland, C. (2007). Clinical neuropathology text and color atlas. Demos medical publishing, LLC, New York.
Isa, M. N., Sulong, S. Sidek, M. R., George, P. J., and Abdullah, J. M. (2003). Telomerase activity in Malaysian patients with central nervous system tumors. Southeast Asian J. Trop. Med. Public Health, 34(4), 872-6
Jafri,A.; Aziz, M.Y.; Ros, S. and I Nizam.(2003). Mutations of the p53 Gene in Gliomas From Malay Patients. Med J Malaysia, 58 ( 2): 236-243.
Keshava R, Jothi M, Gope ML, Gope R.(2010). Functional modulation of the p53 gene and its protein in human brain tumors. Ann Neurosci; 15: 75–86.
Kovach J S, McGovern R M, Cassady J D, Swanson S K, Wold L S, Vogelstein B and Sommer S S. (1991). Direct sequencing from touch preparations of Human carcinomas: Analysis of p53 mutations in Breast carcinoma; J. Natl. Cancer Inst. 83: 1004-1009.
Maniatis, T., Fritch, E. F., and Sambrook, J. (1982). Molecular cloning: A laboratory manual, Cold Spring Harbor Laboratory Press, New York.
Osman, F. A. (2005). Brain tumors. Humana Press Inc.
Patt, S.; Gries, H.; Giraldo, M.; Cervos-Navarro, J.; Martin, H.; Janisch, W. and Brockmoller, J. (1996). p53 Gene mutations in human astrocytic brain tumors including pilocytic astrocytomas. Human Pathology, 27: 586-589.
Phatak, P.; Selvi, S. K.; Divya, T.; Hegde ,A. S., Hegde S. and Somasundaram ,K. (2002). Alterations in tumor suppressor gene p53 in human gliomas from Indian patients. Journal of Bioscience, 27: 673–678.
Quan, A. L.; Barnett, G. H.; Lee, S. Y.; Vogelbaum, M. A.; Toms, S. A.; Staugaitis, S. M.; Prayson, R. A.; Peereboom, D. M.; Stevens, G. H. J.; Cohen, B. H. and Suh, J. H.(2005). Epidermal growth factor receptor amplification does not have prognostic significance in patients with glioblastoma multiforme.
Ruddon, R. W. (2007). Cancer biology. (4th ed ).Oxford University Press, Inc., New York.
Smith, C. and Ironside, J. W. (2007). Diagnosis and pathogenesis of gliomas. Current Diagnostic Pathology, 13, 180–192.
Zupanska, A., and Kaminska, B. (2002). The diversity of p53 mutations among human brain tumors and their functional consequences. Neurochemistry International, 40, 637–645.
Zupanska, A., and Kaminska, B. (2002). The diversity of p53 mutations among human brain tumors and their functional consequences. Neurochemistry International, 40, 637–645.
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Published
2015-09-30
How to Cite
Ameen, N. A., Nerweyi, F. F. A., Sulayman, A., Jubrael, J. T. M. S., Al-Rawi, W. W. H., & Ibrahim, S. H. (2015). Identification of P53 Tumor Suppressor Gene Alterations in Human Glioma Using Sscp-Pcr Technique. Science Journal of University of Zakho, 3(2), 194–198. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/140
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Science Journal of University of Zakho
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