Vasorelaxant and Antispasmodic Effects of Some Polyphenols and Glycoside in Rat Smooth Muscles

Authors

  • Mudhir S. Shekha University of Salahaddin

Keywords:

vasorelaxant, antispasmodic, polyphenol, glycoside, rat, smooth muscles

Abstract

Objective: The present study was undertaken to investigate the effect of Luteolin, chlorogenic acid, amygdalin and tannic acid on vascular smooth muscle contractility.

Materials and Methods: The effect of different concentrations of some polyphenols and glycosides on contractile responses of isolated aorta, ileum and trachea to Acetylcholine, KCl and phenylephrine (PE) were evaluated.

Results: The results of the current study indicated that luteolin and chlorogenic acid has potent relaxant effects on KCl and PE-induced contractions in rat’s aortic ring. Amygdalin and tannic acid reduced ACh-induced ileum contractions significantly. Conclusions: The inhibitory effect of luteolin and chlorogenic acid on the contraction induced by PE and KCl may be due to their anti-adrenergic and anti-hyperpolarizing effect.

Author Biography

Mudhir S. Shekha, University of Salahaddin

Department of Biology, Colleges of Science, University of Salahaddin, Kurdistan Region – Iraq.

References

Andrade R.G., Jr., Dalvi L.T., Silva J.M., Jr., Lopes G.K., Alonso A. & Hermes-Lima M. (2005) The antioxidant effect of tannic acid on the in vitro copper-mediated formation of free radicals. Arch Biochem Biophys 437, 1-9.
Bankar G.R., Nayak P.G., Bansal P., Paul P., Pai K.S., Singla R.K. & Bhat V.G. (2011) Vasorelaxant and antihypertensive effect of Cocos nucifera Linn. endocarp on isolated rat thoracic aorta and DOCA salt-induced hypertensive rats. J References
Andrade R.G., Jr., Dalvi L.T., Silva J.M., Jr., Lopes G.K., Alonso A. & Hermes-Lima M. (2005) The antioxidant effect of tannic acid on the in vitro copper-mediated formation of free radicals. Arch Biochem Biophys 437, 1-9.
Bankar G.R., Nayak P.G., Bansal P., Paul P., Pai K.S., Singla R.K. & Bhat V.G. (2011) Vasorelaxant and antihypertensive effect of Cocos nucifera Linn. endocarp on isolated rat thoracic aorta and DOCA salt-induced hypertensive rats. J Ethnopharmacol 134, 50-4.
Baroni A., Paoletti I., Greco R., Satriano R.A., Ruocco E., Tufano M.A. & Perez J.J. (2005) Immunomodulatory effects of a set of amygdalin analogues on human keratinocyte cells. Experimental Dermatology 14, 854-9.
Calixto J.B., Nicolau M. & Rae G.A. (1986) Pharmacological actions of tannic acid. I. Effects on isolated smooth and cardiac muscles and on blood pressure. Planta Med, 32-5.
Chang K.H., Shin M.-S., Yang H.-Y., Lee J.-W., Kim Y.-S., Lee M.-H., Kim J., Kim K.-H. & Kim C.-J. (2006) Amygdalin induces apoptosis through regulation of Bax and Bcl-2 expressions in human DU145 and LNCaP prostate cancer cells. Biol. Pharm. Bull. 29, 1597– 602.
Chen D., Bi A., Dong X., Jiang Y., Rui B., Liu J., Yin Z. & Luo L. (2014) Luteolin exhibits anti-inflammatory effects by blocking the activity of heat shock protein 90 in macrophages. Biochem Biophys Res Commun 443, 326-32.
Chung K.F. (2008) Airway Smooth Muscle in Asthma and COPD: Biology and Pharmacology. In: Airway Smooth Muscle in Asthma and COPD (pp. 303-12. John Wiley & Sons, Ltd.
Duarte J., Perez Vizcaino F., Utrilla P., Jimenez J., Tamargo J. & Zarzuelo A. (1993) Vasodilatory effects of flavonoids in rat aortic smooth muscle. Structure-activity relationships. Gen Pharmacol 24, 857-62.
Fatehi M., Farifteh F. & Fatehi-Hassanabad Z. (2004) Antispasmodic and hypotensive effects of Ferula asafoetida gum extract. J Ethnopharmacol 91, 321-4.
Fulton D., Mcgiff J.C. & Quilley J. (1996) Cytochrome P450 arachidonate metabolites: deficit in diabetes mellitus. FASEB J 9.
Graf B.A., Milbury P.E. & Blumberg J.B. (2005) Flavonols, flavones, flavanones, and human health: epidemiological evidence. J Med Food 8, 281-90.
Gülçin İ., Huyut Z., Elmastaş M. & Aboul-Enein H.Y. (2010) Radical scavenging and antioxidant activity of tannic acid. Arabian Journal of Chemistry 3, 43-53.
Habauzit V. & Morand C. (2012) Evidence for a protective effect of polyphenols-containing foods on cardiovascular health: an update for clinicians. Therapeutic Advances in Chronic Disease 3, 87-106.
Hwang H.J., Kim P., Kim C.J., Lee H.J., Shim I., Yin C.S., Yang Y. & Hahm D.H. (2008) Antinociceptive effect of amygdalin isolated from Prunus armeniaca on formalin-induced pain in rats. Biol Pharm Bull 31, 1559-64.
Hwang S.J., Kim Y.W., Park Y., Lee H.J. & Kim K.W. (2014) Anti-inflammatory effects of chlorogenic acid in lipopolysaccharide-stimulated RAW 264.7 cells. Inflamm Res 63, 81-90.
Karaki H. & Weiss G.B. (1988) Calcium release in smooth muscle. Life Sci 42, 111-22.
Ko W.C., Shih C.M., Leu I.J., Chen T.T. & Chang J.P. (2005) Mechanisms of relaxant action of luteolin in isolated guinea pig trachea. Planta Med 71, 406-11.
Ethnopharmacol 134, 50-4.
Baroni A., Paoletti I., Greco R., Satriano R.A., Ruocco E., Tufano M.A. & Perez J.J. (2005) Immunomodulatory effects of a set of amygdalin analogues on human keratinocyte cells. Experimental Dermatology 14, 854-9.
Calixto J.B., Nicolau M. & Rae G.A. (1986) Pharmacological actions of tannic acid. I. Effects on isolated smooth and cardiac muscles and on blood pressure. Planta Med, 32-5.
Kumar S.P., Patel J.S. & Saraf M.N. (2008) Mechanism of vasorelaxant activity of a fraction of root extract of Sesamum indicum Linn. Indian J Exp Biol 46, 457-64.
Leblais V., Krisa S., Valls J., Courtois A., Abdelouhab S., Vila A.M., Merillon J.M. & Muller B. (2008) Relaxation induced by red wine polyphenolic compounds in rat pulmonary arteries: lack of inhibition by NO-synthase inhibitor. Fundam Clin Pharmacol 22, 25-35.
Loizzo M.R., Said A., Tundis R., Rashed K., Statti G.A., Hufner A. & Menichini F. (2007) Inhibition of angiotensin converting enzyme (ACE) by flavonoids isolated from Ailanthus excelsa (Roxb) (Simaroubaceae). Phytother Res 21, 32-6.
Menaa F., Menaa A. & Tréton J. (2014) Polyphenols in Human Health and Disease. 1, 819–30.
Namkung W., Thiagarajah J.R., Phuan P.-W. & Verkman A.S. (2010) Inhibition of Ca(2+)-activated Cl(−) channels by gallotannins as a possible molecular basis for health benefits of red wine and green tea. The FASEB Journal 24, 4178-86.
Reddy H., Watson N., Ford A.P.D.W. & Eglen R.M. (1995) Characterization of the interaction between muscarinic M2 receptors and β-adrenoceptor subtypes in guinea-pig isolated ileum. Br J Pharmacol 114, 49-56.
Sato Y., Itagaki S., Kurokawa T., Ogura J., Kobayashi M., Hirano T., Sugawara M. & Iseki K. (2011) In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid. Int J Pharm 403, 136-8.
Shekha M.S. & Al-Habib O.A.M. (2012) Vasorelaxant, antispasmodic and Cardiotonic effect of the Chloroform Fraction of Crataegus aronia on isolated rat muscles. International Journal of Biological and Biomedical Sciences 1, 6-11.
Shi T., Duan Z.H., Papay R., Pluskota E., Gaivin R.J., de la Motte C.A., Plow E.F. & Perez D.M. (2006) Novel alpha1-adrenergic receptor signaling pathways: secreted factors and interactions with the extracellular matrix. Mol Pharmacol 70, 129-42.
Song Y.S. & Park C.M. (2014) Luteolin and luteolin-7-O-glucoside strengthen antioxidative potential through the modulation of Nrf2/MAPK mediated HO-1 signaling cascade in RAW 264.7 cells. Food Chem Toxicol 65, 70-5.
Zhao Y., Wang J., Ballevre O., Luo H. & Zhang W. (2012) Antihypertensive effects and mechanisms of chlorogenic acids. Hypertens Res 35, 370-4

Downloads

Published

2014-12-30

How to Cite

Shekha, M. S. (2014). Vasorelaxant and Antispasmodic Effects of Some Polyphenols and Glycoside in Rat Smooth Muscles. Science Journal of University of Zakho, 2(2), 220–227. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/177

Issue

Section

Science Journal of University of Zakho