Determination of Lipids and Glucose Content in Hydatid Cysts of Echinococcus Granulosus Isolated from Different Intermediate Hosts (Sheep, Goats, Cattle And Human) Tissues
Keywords:
Echinococcosis, Echinococcus granulosus, HCS chemical composition, Lipid, Cholestrole, GlucoseAbstract
This study included comparative biochemical composition of hydatid fluid, protoscolices, infected and non-infected tissues isolated from liver and lungs of infected sheep, goats, and cattle slaughtered in Duhok abattoirs during the period from Nov. 2009 to Apr. 2010. Also hydatid fluid of cysts surgically removed from humans in Azadi Teaching Hospital, Duhok during the period from Mar. 2010 to Jul. 2010. Protoscolices contained higher levels of Lipids, triglycerides and cholesterol with the highest being in cattle liver (63.81±1.434 mg/100g, 38.28±1.277 mgs/100gs and 28.85±0.795 mgs/100gs, respectively) and infected cattle liver and lung tissues (57.78±1.3 mgs/100gs and 51.91±1.299 mgs/100gs, respectively). Hydatid fluid contained high levels of glucose with the highest in hydatid fluid of sheep cysts (Liver: 37.41±0.384 mgs/dl and Lung: 38.98±0.424 mgs/dl) and infected sheep Lung tissues (48.12±0.475 mg/100g).
References
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Thompson, R.C.A., A.J. Lymbery and C.C. Constantine. (1994).Variation in Echinococcosistowards a taxonomic revision of the genus. Advances in Parasitology. 35: 145-176.
Bahr J. M.; Frayha G. J. and Hajjar J. J. (1979). Mechanism of cholesterol absorption by the hydatid cysts of Echinococcus gramdosus (Cestoda). Comparative Biochemistry and Physiology, 62A: 485-490. (Abstract).
Biava, M. F.; Dao, A. and Fortier, B. (2001). Laboratory diagnosis of cystic hydatic disease. World journal of surgery, 25(1): 10-4.
Bowles, J. and McManus, D. P. (1992). Genetic Species within the genus Echinococous identified by mitochondrial DNA sequencing. Mol. Biochem. Parasitol., 54: 156-174.
Bowles, J. and McManus, D. P. (1993). Rapid discrimination of Echinococous species and strains using a PCR-based KELP method. Mol. Biochem. Parasitol., 57: 231-239.
Bowles, J. and McManus, D. P. (1994). Molecular genetic characterization of the Cervid strain (northern form) of Echinococous granulosus. Parasitol., 109: 215-221.
Cohen, B. H. (2003). Calculating a Factorial ANOVA From Means and Standard Deviations. Department of Psychology, New York University, Understanding Statistical, 1(3): 191-203.
Craig, P. S.; Deshan, L.; MacPherson, C. N.; Dazhong, S.; Reynolds, D.; Barnish, G.; Gottstein, B. and Zhirong, W. (1992). A large focus of alveolar Echinococcosis in central China. Lancet, 340: 826-831.
Craig, P. S.; Rogan, M. T. and Allan, J. C. (1996). Detection, screening and community epidemiology of taeniid cestode zoonoses: cystic Echinococcosis, alveolar Echinococcosis and neurocysticercosis. Adv. Parasitol., 38: 169-250.
Dalian, A. and Mobedi, I. (1997). Helminth parasits of Carnivores in northern Iran. Ann. Trop. Med. Parasitol., 86: 395-397.
Eslamirad, Z.; Deylami, A. and Moubedi, A. (2000). Comparison of protein and lipid levels in hepatic hydatid cyst fluid isolated from sheep, cattle and goat. Journal of Ghazvin Medical Science University, 11: 22-26.
Frayha, G. J. and Haddad, R. (1980). Comparative chemical composition of protoscolices and hydatid cyst fluid of Echinococcus granulosus (Cestoda). Int. J. Parasitol., 10: 359-364.
Izadi, J. and Ajami, A. (2006). Biochemical profiles of hydatid cyst fluids of Echinococcus granulosus of human and animal orign (Sheep, Goat, Cattle and Camel). J. Animal Vet. Adv., 5(7): 574-577.
MacPherson, C. N. L.; French, C. M.; Stevenson, L., Karstad, and Arundel, J.H. (1985). Hydatid disease in the Turkana District of Kenya, IV. The prevalence of E. granulosus infection in dog, and observation on the role of the dog in the lifestyle of the Turkana. Ann. Trop. Med. Parasitol., 79:51-61.
McManus, D. P. (1981). Biochemical study of adult and cystic stages of Echinococcus granulosus of human and animal origin from Kenya. J. Helminthes, 55: 21-27.
McManus, D.P. and McPherson, C. (1984). Strain characterization in the Hydatid organism, Echinococcus granulosus. J. Helminthes, 78: 193- 198.
Mero, W.M., Al-Zako, S.S. and Zakaria, S.J. (1988). Comparative study on some enzyme activity in Fasciola hepatica, Fasciola gigantia and Bovine liver. J. Edu. And Sci. Vol. 8. Dep. of Biology, College of Science, University of Mosul- Iraq, p 62-67.
Radfar, M. H. and Iranyar, N. (2004). Biochemical profiles of hydatid cyst fluids of Echinococcus granulosus of human and animal origin in Iran. J. Vet., 4 (6): 435-442.
Refik, M.; Mehmet, N.; Durmaz, B. and Egri, M. (2002). Determination of some biochemical parameters in hydatid cyst fluids. Erciye Medical Journal, 24(1): 10-13.
Saeed, K. H. M. and Al-Habbib, O. A. M. (1990). Practical animal physiology, Dar Al-Hikma, Mosul University, 228.
Shaafie, I.A.; Khan, A., and Rambabu, K. (1999). Biochemical profiles of hydatid cyst fluids of E. granulosus of human and animal origin in Libya. J. Helminthol., 73: 253-258.
Sharif M.; Keighobadi M.; Ziaee H.; Izadi J.; Gholami S.H.; Khalilian A. (2004). Measurement of biochemical components of liver Hydatid cyst fluids in human, sheep, goats, cattle and camel. Mazandaran. Journal of Arak University of Medical Science, 2: 24-31.
Tangprawat, C. (2006). Determination of total lipid content and gamma-oryzanol in rice bran. Science (Public health) major in nutrition faculty of graduate studies Mahidol University. M.Sc. Thesis.
Thompson, R. C. A. (1995). Biology and systematic of Echinococcus. In Echinococcus and hydatid disease (R. C. A. Thompson and A. J. Lymbery, eds). CAB International, Wallingford, 1-50.
Thompson, R.C.A., A.J. Lymbery and C.C. Constantine. (1994).Variation in Echinococcosistowards a taxonomic revision of the genus. Advances in Parasitology. 35: 145-176.
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2013-06-30
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Meerkhan, A. A., & Mero, W. M. (2013). Determination of Lipids and Glucose Content in Hydatid Cysts of Echinococcus Granulosus Isolated from Different Intermediate Hosts (Sheep, Goats, Cattle And Human) Tissues. Science Journal of University of Zakho, 1(1), 50–57. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/36
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