@article{Al Rawi_Al Mudhaffar_Hassan_2013, title={Kinetic and Thermodynamic Studies on Afp Binding to Afp Antibody in Colorectal Tumor Homogenates}, volume={1}, url={https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/372}, abstractNote={<p>Kinetic and thermodynamic parameters associated with the binding of <sup>125</sup>I- anti AFP Antibody to AFP in both crude colorectal homogenate and partially- purified fractions were investigated. It was shown that the reaction in all studied groups follow pseudo- first order reaction kinetics.The value of kinetic parameters K<sub>a</sub>, K<sub>d</sub>, K<sub>obs</sub>, K<sub>+1</sub>, K<sub>-1</sub>, (t<sub>1/2</sub>)<sub> ass.</sub>, (t<sub>1/2</sub>)<sub>diss</sub>. and maximal binding capacity (B<sub>max</sub>) at 25<sup>o</sup>C for the binding of anti <sup>125</sup>I-AFP antibody with its cytosolic antigen in benign colorectal tumor were found to be : 0.0543 x 10<sup>10</sup> M<sup>-1</sup>, 15.064 x 10<sup>-10</sup>M, 0.0158 min<sup>-1</sup>, 4.086 x 10<sup>6</sup> M<sup>-1</sup>.min<sup>-1</sup>, 64.76x10<sup>-4</sup> min<sup>-1</sup>, 43 min, 104.28 min and 29.14 Pmol/mg protein respectively, while 0.0538 x 10<sup>10</sup> M<sup>-1</sup>, 15.142 x 10<sup>-10</sup> M, 0.0163 min<sup>-1</sup>, 4.301 x 10<sup>6</sup> M<sup>-1</sup>.min<sup>-1</sup>, 67.63 x 10<sup>-4</sup> min<sup>-1</sup>, 40 min, 79.66 min and 46.25 Pmol/mg respectively for the binding of anti <sup>125</sup>I-AFP antibody with its cytosolic antigen in tissues of malignant colorectal tumor and at 4<sup>o</sup>C.  The maximum binding of partially purified AFP occurred at 25<sup>o</sup>C. The values ofK<sub>a</sub> and K<sub>+1</sub> increased with increasing temperature. The Van<sup>’</sup>t Hoff plot demonstrated linear relationship between lnKa and 1/T, using crude colorectal homogenate partially purified AFP as AFP source. Plotting between ln K<sub>+1</sub> and 1/T gave linear relationship called Arrheniuns relationship. The thermodynamic parameters ΔΗ˚, ΔG˚ and ΔЅ˚ for the formation of (<sup>125</sup>I- anti AFP Antibody / AFP) complex at the standard state had been determinedas well asEa, ΔΗ*, ΔG* and ΔЅ* which representing the transition state.</p>}, number={2}, journal={Science Journal of University of Zakho}, author={Al Rawi, Bilal J. M. and Al Mudhaffar, Sami A. and Hassan, Hathama R.}, year={2013}, month={Sep.}, pages={699–714} }