TY - JOUR AU - Mohammed, Ahmed B. AU - Mustafa, Awat AU - Issa, Araz R. AU - Mero, Wijdan M.S. PY - 2016/06/30 Y2 - 2024/03/29 TI - Molecular Characterization of Fasciola Spp. Isolated From the Gallbladder of Infected Cattle in Duhok Province, Kurdistan Region Iraq JF - Science Journal of University of Zakho JA - SJUOZ VL - 4 IS - 1 SE - DO - UR - https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/303 SP - 37 - 42 AB - <p><em>Fascioliasis</em>&nbsp;caused by the liver flukes of the genus&nbsp;<em>Fasciola</em>&nbsp;is considered as the most significant Trematodes infection of ruminants in both temperate and tropical countries. In the present study fifty adult&nbsp;<em>Fasciola&nbsp;</em>flukes (Platyhelminthes: Trematoda: Digenia) were collected from the bile ducts and gallbladder of infected bovine hosts (cattle) slaughtered at Duhok abattoir, Kurdistan region, Iraq.&nbsp; Genomic DNA extraction was performed using a Genomic DNA Extraction kit. ITS-1 and ITS-2 ribosomal DNA sequences have been used to characterize these liver flukes as a specific marker. The PCR products were separated by electrophoresis in 1.5% agarose gel, visualized by staining with ethidium bromide, and photographed. ITS-2 marker for&nbsp;<em>F. hepatica</em>&nbsp;was amplified successfully and the length of produced band for ITS-2 was 330 bp. The present study is the first trail for molecular characterization of&nbsp;<em>F. hepatica</em>&nbsp;in cattle in Duhok, Kurdistan Region, Iraq &nbsp;using the ITS-2 rDNA as a reliable genetic marker.</p> ER -