TY - JOUR AU - Khalid, Haval M. AU - Yassin, Najim A. PY - 2017/03/30 Y2 - 2024/03/29 TI - Distribution of Extended Spectrum Β-Lactamase Genes among Proteus Mirabilis Isolated From Clinical Specimens in Duhok City, Kurdistan Region, Iraq JF - Science Journal of University of Zakho JA - SJUOZ VL - 5 IS - 1 SE - DO - 10.25271/2017.5.1.290 UR - https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/444 SP - 1-6 AB - <p>Extended Spectrum Beta Lactamase-among&nbsp;<em>Proteus mirabilis</em>&nbsp;strains recorded high incidence leaving few therapeutic options of potential infections. The purpose of current study was to assess the prevalence of antibiotic resistance among Extended Spectrum Beta-Lactamases (ESBL) producing&nbsp;<em>P. mirabilis</em>, in addition to molecular characterization of the ESBL gene-types using PCR. All isolates were fully identified, checked for antibiotic susceptibility and ESBL production using double disk synergy phenotypic method. Positive ESBL-producing isolates were subjected to PCR assay using specific primers for detection of CTX-M, TEM, and SHV genes. The majority of the isolates exhibited absolute susceptibility (100%) to both meropenem and ertapenem and high susceptibility (95%) to imipenem, while co-resistance were expressed toward cefotaxime, ceftazidime, ceftriaxone and other non-lactam antibiotics. Out of 37 isolates, 21(57%) were ESBL-producers and using a double-disc synergy test (DDST). Using molecular-based PCR, CTX-M (81%), TEM (57%) and SHV (24%) were determined among ESBL-positive. CTX-M was predominant and circulating among phenotypic multiple resistant strains. Moreover, the coexistence of CTX-M and TEM gene was a more frequent combination. The study highlighted the increasing levels of low antibiotic susceptibility among&nbsp;<em>P. mirabilis</em>&nbsp;harbored ESBL genes at Duhok city and also confirms that a high level of&nbsp;<em>bla</em>CTX-M-positive ESBL isolates is circulating in this area.</p> ER -