Effect of Some Chemical Factors on Pullulan Production from Date Syrup by Alocal Isolate of the Fungus Aurobisedium Pullulans
DOI:
https://doi.org/10.25271/2017.5.1.294Keywords:
Date syrup, Aeurobasidium, Exopolysaccharide, pullulanAbstract
This study was developed to use Iraqi date syrup as a carbon source and basal substrate for the production of the exopolysaccharide “Pullulan” by the yeast like fungus A. pullulans. It has been revealed that the best incubation period for high accumulation of pullulan was three days of incubation with initial pH value of 6.5. Also, the fermentation medium of 12% sugar was found to be superior to other sugar concentrations with respect to pullulan production. Moreover, the results of the addition of different nitrogen source containing same amounts of nitrogen present in 0.2% ammonium sulphate show that the urea stimulated pullulan biosynthesis by the fungus more than other tested nitrogen sources. Additionally, highest production of pullulan was obtained when a concentration of 0.2% sodium chloride and was added to the fermentation medium.
References
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Choudhury, A. R., Bhattacharyya, M. S., and Prasad, G. S., (2012). application of response surface methodology to understand the interaction of media component during pullulan production by Aureobasidium Pullulans RBF-4A3, Biocatalysts Agr. Biotechnol. 1:232-237.
Dubios, M. K. A., Gilles, J. K., Hamitton, P. A., Aobers, and smith, F. (1955). Colorimetric method for the determination of sugars, Anal. Chem., 28: 350-356.
Gniewosz, M. E. , Sobczak, and WZielinsk (1997). Optimization of sacchrose and Ammonium sulfate concentration for pullulan biosynthesis by Aureobasidium Pullulans in batch culture. Microbiol., 6:61-68.
Goksunger, Y. and Guvenc, U. (2004). Production of pullulan from beet molasses and synthesis medium by Aureobasidium pullulans.Biol., 28:23-30.
Hamer, B. and West, P. (1993). Effect of temperature on pullulan production in relation to carbon source, Microbiol.,80: 83-90.
Hamer, B. and West, P (1994). Effect of complex nitrogen source on pullulan Production relative to carbon source, Microbiol., 80:83-90.
Imshenetskii, A. A. T. F., Kondrateva, and Sumuko, A.N. (1981). Influence of the acidity of the medium, condition of aeration of the medium, condition of aeration, and temperature on pullulan biosynthesis by polyploidy strains of Pullularia Aureobasidium pullulans, Microbiol.,50: 471-479.
Kim J.H., Kim M.R., Lee J.H., Lee J.W., Kim S.K. (2000). Production of high molecural weight pullulan by Aureobasidium pullulans using glucosamine. Biotechnol. Lett. 22: 987-990.
Lazaridou, G., Costas, and Biliaderis .(2002). Production and characterization of Pullulan from beet molasses using anon pigment strain of Aureobasidium Pullulans in batch culture.Apple. Biochem. Biotechnol., 97:1385-1392.
Leathers, T. and Timothy, D. (2004).Characterization of exopolysaccharides Produced from Aureobasidium Pullulans. Biotechnol. Bioeng.72:161-169
Ma, J., Jiang, G., Yao, S., Jin, H. and Wang, C. (2012). Studies on the optimal culture conditions of Aureobasidium pullulans to produce exopolysaccharides, J. Biomed. Sci. Eng., 5:203-207.
McNeil, B., Kristiansen B. (1987). Influence of impeller speed upon the pullulan fermentation. Biotechnol. Lett. 9: 101-104.
Mishra B. M., Vuppu S., Rath, K.(2011). The role of microbial pullulan, a biopolymer in pharmaceutical approaches: A review. J. Appl. Pharm. Sci. 1: 45-50.
Najum, N. M. (2008). Production of pullulan from date extract by the fungus A. pullulans ATCC 16628. M. Sc. Thesis, university of Garyounis, Benghazi, Libya.
Oliveira, J. D., Lopes, L. M., Pimenta, F. D. and Servulo, E. F. (2015). Low cost production of pullulan obtained from granulated sugar and different nitrogen sources, Int. J. Eng. Technol., 15:11-17.
Peters, H. P. F., Ravestein P., van der Hijden H.T.W.M., Boers H.M., Mela D.J. (2011). Effect of carbohydrate digestibility on appetite and its relationship to postprandial blood glucose and insulin levels. Eur. J. Clin. Nutr.65: 47-54.
Rho D., Mulchandali A., Luong J.H.T., Leduy A. (1988). Oxygen requirement in pullulan fermentation. Appl. Microbiol. Biotechnol. 28, 361 -366.
Roukas T. (1999). Pullulan production from brewery wastes by Aureobasidium pullulans. World J. Microbiol. Biotechnol.15: 447-450.
Roukas T., Biliaderis C.G. (1995). Evaluation of carob pod as a substrate for pullulan production by Aureobasidium pullulans. Appl. Biochem. Biotechnol.55: 27-44.
Sena, R. F., Costeli, M. C., Gibson, L. H., and Coughlin, R. W., (2006). enhanced production of pullulan by two strains of Aureobasidium Pullulans with different concentration of soybean oil in source solution in batch fermentation. Chemical. Engineering,23:507-515.
Sharma, G., Muthukumaran, C., Nayan, G. and Nidhi, B. (2013). Extracellular biopolymer production by Aureobasidium pullulans MTCC2195 using jackfruit seeds powder, J. Polym. Env., 21: 487-494.
Singh, R. ,Gaur, R., Tiwari, S. and Gaur, M. K. (2012). Production of pullulan by a Thermotolerant Aureobasidium Pullulans strain in non-stirred fed batch Fermentation process, Brazilian J. Microbiol., 43: 1042-1050.
Smith, A., and Pace G. W. (1982). Recovery of microbial polysaccharide. J. Chem. Technol. Biotechnol., 32:119-129.
Sobczak, E., and Gniewosz, M., (1996). Molasses brew applied in the production of pullulan by Aureobasidium Pullulans. Food and Nutrition Sci., 5:17-25.
Taguchi R., Kikuchi Y., Sakano Y., Kobayashi T., 1973. Structural uniformity of pullulan produced by several strains of Pullularia pullulans. Agr. Biol. Chem. 37, 1583-1588.
Timothy, D., Leather, T. D., and Subhash, C. (1994). Production of pullulan from fuel ethanol by products by Aureobasidium pullulans, Biotechnol., Lett., 16:1163-1166.
Urkut, Z. (2007). Optimization of pullulan production by using –Ca-alaginate immobilized Aureobasidium Pullulans P56. Ege University Institute of science. Master Thesis ,pp 125, Bornova-Izmir, Turkey.
West, T. P. and B. Reed-Hamer (1999). Ability of Aureobasidium Pullulans to Synthesize pullulan upon selected sources of carbon and nitrogen Microbiol., 67:117-124.
West, T. P. and Hamer, B. (1995). Effect of oils and surfactants on pullulan Production relative on nitrogen source, Microbiol., 83:249-259.
West, T. P. (2011). Effect of carbon source on polysaccharide production by alginate –entrapped Aureobasidium Pullulans ATCC 42023 cells. J Basic Microbiol. 40:10: 1002-1008.
Wolf B.W., Garleb K.A., Choe Y.S., Humphrey P.M., Maki K.C., 2003. Pullulan is a slowly digested carbohydrate in humans. J. Nutr. 133, 1051-1055.
Yousif, F., Roukas, T., and Biliaderis, C. G., (1999). Pullulan production by a non-pigment strain of Aureobasidium Pullulans using batch and fed-batch culture.process Biochem., 34:355-366.
Cheng K.C., Demirci A., Catchmark J.M. (2011). Pullulan: biosynthesis, production and applications. Appl. Microbiol. Biotechnol. 92: 29-44.
Choudhury, A. R., Bhattacharyya, M. S., and Prasad, G. S., (2012). application of response surface methodology to understand the interaction of media component during pullulan production by Aureobasidium Pullulans RBF-4A3, Biocatalysts Agr. Biotechnol. 1:232-237.
Dubios, M. K. A., Gilles, J. K., Hamitton, P. A., Aobers, and smith, F. (1955). Colorimetric method for the determination of sugars, Anal. Chem., 28: 350-356.
Gniewosz, M. E. , Sobczak, and WZielinsk (1997). Optimization of sacchrose and Ammonium sulfate concentration for pullulan biosynthesis by Aureobasidium Pullulans in batch culture. Microbiol., 6:61-68.
Goksunger, Y. and Guvenc, U. (2004). Production of pullulan from beet molasses and synthesis medium by Aureobasidium pullulans.Biol., 28:23-30.
Hamer, B. and West, P. (1993). Effect of temperature on pullulan production in relation to carbon source, Microbiol.,80: 83-90.
Hamer, B. and West, P (1994). Effect of complex nitrogen source on pullulan Production relative to carbon source, Microbiol., 80:83-90.
Imshenetskii, A. A. T. F., Kondrateva, and Sumuko, A.N. (1981). Influence of the acidity of the medium, condition of aeration of the medium, condition of aeration, and temperature on pullulan biosynthesis by polyploidy strains of Pullularia Aureobasidium pullulans, Microbiol.,50: 471-479.
Kim J.H., Kim M.R., Lee J.H., Lee J.W., Kim S.K. (2000). Production of high molecural weight pullulan by Aureobasidium pullulans using glucosamine. Biotechnol. Lett. 22: 987-990.
Lazaridou, G., Costas, and Biliaderis .(2002). Production and characterization of Pullulan from beet molasses using anon pigment strain of Aureobasidium Pullulans in batch culture.Apple. Biochem. Biotechnol., 97:1385-1392.
Leathers, T. and Timothy, D. (2004).Characterization of exopolysaccharides Produced from Aureobasidium Pullulans. Biotechnol. Bioeng.72:161-169
Ma, J., Jiang, G., Yao, S., Jin, H. and Wang, C. (2012). Studies on the optimal culture conditions of Aureobasidium pullulans to produce exopolysaccharides, J. Biomed. Sci. Eng., 5:203-207.
McNeil, B., Kristiansen B. (1987). Influence of impeller speed upon the pullulan fermentation. Biotechnol. Lett. 9: 101-104.
Mishra B. M., Vuppu S., Rath, K.(2011). The role of microbial pullulan, a biopolymer in pharmaceutical approaches: A review. J. Appl. Pharm. Sci. 1: 45-50.
Najum, N. M. (2008). Production of pullulan from date extract by the fungus A. pullulans ATCC 16628. M. Sc. Thesis, university of Garyounis, Benghazi, Libya.
Oliveira, J. D., Lopes, L. M., Pimenta, F. D. and Servulo, E. F. (2015). Low cost production of pullulan obtained from granulated sugar and different nitrogen sources, Int. J. Eng. Technol., 15:11-17.
Peters, H. P. F., Ravestein P., van der Hijden H.T.W.M., Boers H.M., Mela D.J. (2011). Effect of carbohydrate digestibility on appetite and its relationship to postprandial blood glucose and insulin levels. Eur. J. Clin. Nutr.65: 47-54.
Rho D., Mulchandali A., Luong J.H.T., Leduy A. (1988). Oxygen requirement in pullulan fermentation. Appl. Microbiol. Biotechnol. 28, 361 -366.
Roukas T. (1999). Pullulan production from brewery wastes by Aureobasidium pullulans. World J. Microbiol. Biotechnol.15: 447-450.
Roukas T., Biliaderis C.G. (1995). Evaluation of carob pod as a substrate for pullulan production by Aureobasidium pullulans. Appl. Biochem. Biotechnol.55: 27-44.
Sena, R. F., Costeli, M. C., Gibson, L. H., and Coughlin, R. W., (2006). enhanced production of pullulan by two strains of Aureobasidium Pullulans with different concentration of soybean oil in source solution in batch fermentation. Chemical. Engineering,23:507-515.
Sharma, G., Muthukumaran, C., Nayan, G. and Nidhi, B. (2013). Extracellular biopolymer production by Aureobasidium pullulans MTCC2195 using jackfruit seeds powder, J. Polym. Env., 21: 487-494.
Singh, R. ,Gaur, R., Tiwari, S. and Gaur, M. K. (2012). Production of pullulan by a Thermotolerant Aureobasidium Pullulans strain in non-stirred fed batch Fermentation process, Brazilian J. Microbiol., 43: 1042-1050.
Smith, A., and Pace G. W. (1982). Recovery of microbial polysaccharide. J. Chem. Technol. Biotechnol., 32:119-129.
Sobczak, E., and Gniewosz, M., (1996). Molasses brew applied in the production of pullulan by Aureobasidium Pullulans. Food and Nutrition Sci., 5:17-25.
Taguchi R., Kikuchi Y., Sakano Y., Kobayashi T., 1973. Structural uniformity of pullulan produced by several strains of Pullularia pullulans. Agr. Biol. Chem. 37, 1583-1588.
Timothy, D., Leather, T. D., and Subhash, C. (1994). Production of pullulan from fuel ethanol by products by Aureobasidium pullulans, Biotechnol., Lett., 16:1163-1166.
Urkut, Z. (2007). Optimization of pullulan production by using –Ca-alaginate immobilized Aureobasidium Pullulans P56. Ege University Institute of science. Master Thesis ,pp 125, Bornova-Izmir, Turkey.
West, T. P. and B. Reed-Hamer (1999). Ability of Aureobasidium Pullulans to Synthesize pullulan upon selected sources of carbon and nitrogen Microbiol., 67:117-124.
West, T. P. and Hamer, B. (1995). Effect of oils and surfactants on pullulan Production relative on nitrogen source, Microbiol., 83:249-259.
West, T. P. (2011). Effect of carbon source on polysaccharide production by alginate –entrapped Aureobasidium Pullulans ATCC 42023 cells. J Basic Microbiol. 40:10: 1002-1008.
Wolf B.W., Garleb K.A., Choe Y.S., Humphrey P.M., Maki K.C., 2003. Pullulan is a slowly digested carbohydrate in humans. J. Nutr. 133, 1051-1055.
Yousif, F., Roukas, T., and Biliaderis, C. G., (1999). Pullulan production by a non-pigment strain of Aureobasidium Pullulans using batch and fed-batch culture.process Biochem., 34:355-366.
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2017-03-30
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Haidar, M. M., & Adil, J. N. (2017). Effect of Some Chemical Factors on Pullulan Production from Date Syrup by Alocal Isolate of the Fungus Aurobisedium Pullulans. Science Journal of University of Zakho, 5(1), 20–24. https://doi.org/10.25271/2017.5.1.294
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