DEVELOPING AND VALIDATING A STABILITY-INDICATING UV-VIS NANODROP 2000C METHOD FOR THE SIMULTANEOUS DETERMINATION OF THE ANTI-HYPERTENSIVE DRUG HYDROCHLOROTHIAZIDE (HCTZ) IN BOTH BULK AND TABLET DOSAGE FORMS

Authors

  • Faroq O. Qasim Department of Horticulture, Technical College of Akre, Akre University for Applied Sciences, Kurdistan Region, Iraq
  • Mohammed A. Hami Chemistry Department, College of Science, University of Zakho, Kurdistan-Region, Iraq
  • Nzar I. Yousif Department of Medical Laboratory Science, College of Science, Cihan University-Erbil, Kurdistan Region, Iraq

DOI:

https://doi.org/10.25271/sjuoz.2024.12.3.1324

Keywords:

UV-Vis Nanodrop 2000c, Validation, Hydrochlorothiazide, Stability Indicating, Hypertension

Abstract

For the simultaneous determination of anti-hypertensive drugs Hydrochlorothiazide (HCTZ) in bulk and tablet dosage forms, a simple, specific, accurate, and precise stability-indicating UV-Vis Nanodrop 2000c method was developed and validated. The maximum absorption of Hydrochlorothiazide was revealed at 271 nm using methanol as a solvent. The validation results showed good linearity (R2 = 0.9997) within the concentration range of 10-50µg/mL. The RSD revealed an acceptable value (less than 0.6 percent) in the precision study at three levels. 1.01 and 3.3g µg/mL were found to represent the LOD and LOQ, respectively. Locally and commercial tablets are Available Drug Products. Accuracy at three levels showed good recovery, more than 99% percent. The proposed method was used to analyse the stability of local and commercial tablets. The stability of their pharmaceuticals was investigated under acidic, oxidative, thermal, alkaline, and photolytic conditions in this research. In accordance with the guidelines set by the International Council for Harmonisation (ICH), the method was effectively validated. Subsequently, the validated method was employed to analyze commercially available pharmaceutical dosage forms.

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Published

2024-08-15

How to Cite

Qasim, F. O., Hami , M. A., & Yousif , N. I. (2024). DEVELOPING AND VALIDATING A STABILITY-INDICATING UV-VIS NANODROP 2000C METHOD FOR THE SIMULTANEOUS DETERMINATION OF THE ANTI-HYPERTENSIVE DRUG HYDROCHLOROTHIAZIDE (HCTZ) IN BOTH BULK AND TABLET DOSAGE FORMS. Science Journal of University of Zakho, 12(3), 375–381. https://doi.org/10.25271/sjuoz.2024.12.3.1324

Issue

Vol. 12 No. 3 (2024): July-September issue

Section

Science Journal of University of Zakho

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Copyright (c) 2024 Faroq O. Qasim, Mohammed A. Hami , Nzar I. Yousif

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