Histopathological Study of Some Organs After Long-Term Treatment With Dexamethasone in Male Rabbits.
Keywords:
Dexamethasone, histopathology, adrenal gland, Kidney, liverAbstract
The purpose of the study was to determined the histopathological changes in the adrenal gland, Kidney and liver which results from treatment with dexamethasone(DEX) .Thirty local male rabbits in Basra city were divided into three groups,the first group control group was given the normal saline orally , group two were orally given DEX. (0.2 mg/ k g/ BW) and the third group was treated with oral administration of DEX.at a dose of (1 mg. / k g/ BW) for (60) days. The results showed several histopathological changes in the adrenal gland of rabbits in both treated groups represented by congestion of blood vessels, degeneration, vaculation and necrosis of cells in the zona glomerulosa and zona fasciculata of the adrenal cortex. In treated rabbits,the histological section of kidney show some changes represented by degeneration, edema and cells necrosis of epithelial lining of convoluted tubules, infiltration of inflammatory cells and atrophy of some glomeruli. Liver of treated rabbits show ballooning hepatocytes and extension the sinuses.
References
of other. Many fields of liver histological sections revealed cirrhotic and fatty changes.
These results were in agreement with (Kim and shin,1998), who note the ballooning of hepatocytes at mid-and periportal zones in dog liver treated with dexamethasone and explain that due to the accumulation of intracellular fluids which caused hydropic swelling.
References
Adachi, J.D., and papaioannou, A.(2001).Corticosteroid induced osteoporosis : Detection and management .Drug safety,24:607-624.
Bennett, P. and Brown, M. (2003). Clinical pharmacology. 9th. (eds.). Churchill Livingstone., P:663- 675.
Cunha CF, Silva IN, Finch FL (2004). Early adrenocortical recovery after glucocorticoid therapy in childre with leukemia. J. Clin. Endocrinol. Metab. 89: 2797-2802.
Ganong, W.F.(2003). Review of Medical Physiology. 21st ed. McGraw Hill Company, New York, U.S.A.
Gyton, A.C. and Hall, J.E. (2004). Text book of Medical physiology 11th edition press :Elsevier Saunders.
Hemmaid, K. Z. (2009). Ultrastructural patterns of the Adrenal cortical cells of rats during suppression of secretion by dexamethasone injection. Fourth Environmental Conference. Faculty of Science. Zagazig University. 65-83.
House L.and Hill J.,(2002).Textbook of rabbit medicine .Agency Ltd, 90 Tottenham court Road, London, England.
Katzung, B. (2007). Basic pharmacology. 10th. (eds.). Mc Graw Hill. USA
Kim, M. and Shin, H.K. (1998). The water-soluble extract of chicory influences serum and liver lipid concentrations, cecal short-chain fatty acid concentrations and fecal lipid excretion in rats. J. Nutr., 128: 1731-1736.
Latif, D. A. (2010). Immunoprotective Effect of Nigella sativa seed extract in Male Rabbits treated with Dexamethasone. Thesis, College of Vet-Medicine University of Al-Qadisiya.
Luna, L. (1968). Manual of Histological staining Methods. The armed Forces institute of pathology. 3ed. (eds.). Mc Grow- Hill. USA., P:12- 13.
Margarita, F.C., Ivan, V., Manuel, P. and Radu, R. (2006). The effects of sympathoectomy and dexamethasone in rats ingesting sucarose. Int. J. Biol. Sci., 2:17-22.
Messer, J. Reitman, D, Sacks, H. S. Smith, H. J. and Chalmers, T.C. (1983). Association of adrenocorticosteroid therapy and peptic-ulcer disease. N. Engl. J. Med. 309: 21-24.
Mitani F, Mukai K, Miyamoto H, Suematsu M and Ishimura Y( 2003). The undifferentiated cell zone is a stem cell zone in adult rat adrenal cortex. Biochimica et Biophysica Acta 1619 317–324.
Mughal IA, Qureshi AS and Tahir MS (2004). Some histological observations on postnatal growth of rat adrenal gland with advancing age (AHRLM Study). Int.
Ranta F.,Avram D.,Berchtold S.,Dufer M.,Drews G.,Lang F. and Ullrich S.(2006).Dexamethasone induces cell death in insulin-secreting cell,an effect reversed by exendin-4.American Diabetes Association Journal vol.55.
Severino, C., Brizzi, P., Solinas, A.,Secchi, G.,Maioli, M. and Tonolo, G. (2002). Lowdose dexamethasone in the rat: a model to study insulin resistance. Am. J. Physiol. Endocrinol. Metab., 283:367-373.
Van, L.(1994). Dexamethason. Institute of Public Health and Environmental Protection. Bilthoven, Netherlands.
Wang W.,Hullinger R.L.,Andrisani O.M.,(2008).Hepatitis B virus x protein via the p38MApk pathway induces E2F1 release and ATR Kinase activation mediating p53 Apoptosis.The Journal of Biological Chemistary Vol.283 No.37,pp25455-25467 USA
These results were in agreement with (Kim and shin,1998), who note the ballooning of hepatocytes at mid-and periportal zones in dog liver treated with dexamethasone and explain that due to the accumulation of intracellular fluids which caused hydropic swelling.
References
Adachi, J.D., and papaioannou, A.(2001).Corticosteroid induced osteoporosis : Detection and management .Drug safety,24:607-624.
Bennett, P. and Brown, M. (2003). Clinical pharmacology. 9th. (eds.). Churchill Livingstone., P:663- 675.
Cunha CF, Silva IN, Finch FL (2004). Early adrenocortical recovery after glucocorticoid therapy in childre with leukemia. J. Clin. Endocrinol. Metab. 89: 2797-2802.
Ganong, W.F.(2003). Review of Medical Physiology. 21st ed. McGraw Hill Company, New York, U.S.A.
Gyton, A.C. and Hall, J.E. (2004). Text book of Medical physiology 11th edition press :Elsevier Saunders.
Hemmaid, K. Z. (2009). Ultrastructural patterns of the Adrenal cortical cells of rats during suppression of secretion by dexamethasone injection. Fourth Environmental Conference. Faculty of Science. Zagazig University. 65-83.
House L.and Hill J.,(2002).Textbook of rabbit medicine .Agency Ltd, 90 Tottenham court Road, London, England.
Katzung, B. (2007). Basic pharmacology. 10th. (eds.). Mc Graw Hill. USA
Kim, M. and Shin, H.K. (1998). The water-soluble extract of chicory influences serum and liver lipid concentrations, cecal short-chain fatty acid concentrations and fecal lipid excretion in rats. J. Nutr., 128: 1731-1736.
Latif, D. A. (2010). Immunoprotective Effect of Nigella sativa seed extract in Male Rabbits treated with Dexamethasone. Thesis, College of Vet-Medicine University of Al-Qadisiya.
Luna, L. (1968). Manual of Histological staining Methods. The armed Forces institute of pathology. 3ed. (eds.). Mc Grow- Hill. USA., P:12- 13.
Margarita, F.C., Ivan, V., Manuel, P. and Radu, R. (2006). The effects of sympathoectomy and dexamethasone in rats ingesting sucarose. Int. J. Biol. Sci., 2:17-22.
Messer, J. Reitman, D, Sacks, H. S. Smith, H. J. and Chalmers, T.C. (1983). Association of adrenocorticosteroid therapy and peptic-ulcer disease. N. Engl. J. Med. 309: 21-24.
Mitani F, Mukai K, Miyamoto H, Suematsu M and Ishimura Y( 2003). The undifferentiated cell zone is a stem cell zone in adult rat adrenal cortex. Biochimica et Biophysica Acta 1619 317–324.
Mughal IA, Qureshi AS and Tahir MS (2004). Some histological observations on postnatal growth of rat adrenal gland with advancing age (AHRLM Study). Int.
Ranta F.,Avram D.,Berchtold S.,Dufer M.,Drews G.,Lang F. and Ullrich S.(2006).Dexamethasone induces cell death in insulin-secreting cell,an effect reversed by exendin-4.American Diabetes Association Journal vol.55.
Severino, C., Brizzi, P., Solinas, A.,Secchi, G.,Maioli, M. and Tonolo, G. (2002). Lowdose dexamethasone in the rat: a model to study insulin resistance. Am. J. Physiol. Endocrinol. Metab., 283:367-373.
Van, L.(1994). Dexamethason. Institute of Public Health and Environmental Protection. Bilthoven, Netherlands.
Wang W.,Hullinger R.L.,Andrisani O.M.,(2008).Hepatitis B virus x protein via the p38MApk pathway induces E2F1 release and ATR Kinase activation mediating p53 Apoptosis.The Journal of Biological Chemistary Vol.283 No.37,pp25455-25467 USA
Published
2014-06-30
How to Cite
Hussein, A. J., Majeed, M. F., & Abbas, A. S. (2014). Histopathological Study of Some Organs After Long-Term Treatment With Dexamethasone in Male Rabbits. Science Journal of University of Zakho, 2(1), 39–48. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/99
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