Effect of He-Ne Laser on Blood Serum Testosterone and Testicular Tissue in Adult Male Rat
Keywords:
He-Ne laser, Rats, TestosteroneAbstract
The current study was conducted to examine the effect of He-Ne laser therapy on the blood serum testosterone level and testicular tissue in adult male rats. Thirty five Albino Western adult male rats aged 3-4 months and weighing approximately 250-300 g were used and divided in to three groups. The testicular tissue of rats in the first treatment was exposed to a dose of irradiation 1.02 j/cm2 (40 second) once daily for three successively days, while second treatment was exposed to a dose of irradiation 2.03 j/cm2 (80 second) once daily for three successive date, while the third group remained without any treatments (control). The results showed that the process of irradiation adversely affected on the level of blood serum testosterone in the first and second treatment compared to the normal level in the control group. The histological examination in treatment one showed low reduction in numbers of sertoli , leydig and spermatid cells at day one, while in day two showed medium reduction in numbers of sertoli , leydig and spermatid cells,and high reduction in numbers of sertoli , leydig and spermatid cells in day three of irradiation. In treatment two, the results showed medium reduction in numbers of sertoli , leydig and spermatid cells at day one, while in day two showed high reduction in numbers of sertoli , leydig and spermatid cells and very high reduction in numbers of sertoli , leydig and spermatid cells in day three of irradiation . In conclusion the current study revealed that steers factor cause reduction in numbers of sertoli , leydig and spermatid cells lead to low fertility rate within increasing of duration and repetition of irradiation.
References
Arthur. G.H. Noaks .D.E. Pearson. H. and Parkinson.T.J (1998).Veterinary reproduction and obstetrics seventh Ed.W.B. Sounders company limited 553-559.
Bartke, A. R.E. Steel, N. Musto and B.V. Calewell. (1973). Fluctuation in plasma testosterone levels in Adult Male Rats and mice .Endocrinology.92 (4): 1223-1228.
Drury, R.A., Wallington, E.A. and Cameron, S.R (1976). Carletons histological technique , 4th ed. Oxford University press. London: 35,49-59.
Huyan, Si-Le Ren-yu. (1986). Effects of irradiation to the rat scrotal testes on spermatogenesis with Nd:YAG laser. Chinese Journal of Biomedical Engineering- 04.
Knoll BW (1991). Stress and the endocrine hypothalamus pituitary testis system. A review Vet.Q; 13 (2):104-14.
Mailankot, M., Kunnath, A.B.,Tayalesksmih, Kodurn B. andValsalon, R. (2009). Radio frequency Electromagnetic Irradiation (free EMR) from gsm (0.9/1.8 ghz) Mobile Phones Induced Oxidative Stress Reduces Sperm Motility. Clinics, 64(6):561-565.
Meikha, N.H. (2005). Stimulation Burn Healing Using 790 Diode laser in Rabbits. M.Sc. Thesis. Laser Institute for Higher Education. University of Baghdad.
Omran, M.F., ABU-ZIED, N.M. and YACOB, S.F.( 2001). Role of serotonin in the regulation of hormonal and antioxidant defense system in male rats exposed to laser radiation .National center for radiation research and technology, AEA, Cairo Egypt. (on line)
Orr, T.E. and Mann, D.R. (1992) Role of Glucocorticoids in the stress induced suppression of testicular steroidogeness in adult male rats. Hormone and Behavior; 26(3):350-63
Pollard lrine ,BassettJR,and Joss M.P.Jean. (1980). Plasma testosterone levels and 3B-hydroxysteroid-dehydrogness activity in the testis of the rat following prolonged exposure to stress. Journal of reproduction and fertility; 59: 101-106.
Rai ,Jyti;Pandey,S.N. and Srivasatava R.K. (2003). Effect of immoliziationstresson spermatogenesis of albino Rats. J Ant .Soc.India 52 (1) 55-57.
Taha, M.F. and Valojerdi, M.R. (2004). Quantitative and qualitative changes of the seminiferous epithelium induced by Ca. Al. As (830 nm) laser radiation. Lasers surgery and medicine. 34:352-359
William H. Walker and Jing Cheng. (2005). FSH and testosterone signaling in sertoli cells Repoductive J1,130:15-28.
Zhu Jiuming. (1989). Laser Irradiation Effect on Sperm Motility and Viability of Sheep. Application of Laser Issue.
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