Cytogenetic Effects of Honey Contaminated With Fumagillin (Dicyclohexylamine) on Male Mice Mus Musculus Balb/C In Vivo
Keywords:
TheAbstract
Cytogenetic effects of honey contaminated with fumagillin that collected from different sources in Duhok province was investigated in mouse bone-marrow cells using damaged cells%, (chromatids and chromosomal aberrations and mitotic index (M.I). A group of mice was orally administrated with honey that gave a positive result with qualitative chemical test. Fumagillin was administered to another group of mice by gavage, at doses of 25, 50, 75 mg/kg body weight (b.w) prepared with honey that give a negative result by biochemical test as artificially contaminant honey. All mice were treated for two different periods, 7 and 35 days at 24-hrs intervals .The treated groups were compared with negative control and Cyclophosphamide (40 mg/kg bw) as a positive control. The biochemical test for all honey samples shows that 16.67% of honey samples were contaminated with fumagillin. The honey sample that give a positive result in the presence of fumagillin in biochemical test revealed its ability to increase the damage cell% and chromosomal aberrations in bone marrow cells after 7 and 35 days of treatment .The ability of this sample equivalent to that of honey experimentally contaminated with fumagillin (25 mg/kg b.w).The result of the present study shows that the contaminated honey sample revealed its ability to reduce the M.I after 35 days of treatment in bone marrow cells as compared to negative controls.
References
Allsop, K.A. and Miller, J.B. (1996). Honey revisited: A reappraisal of honey in pre-industrial13 diets. Br J Nutr ,75:513-520.
Birch, A. J. and Hussain S. F. (1969).Studies in relation to biosynthesis.Part 38.A preliminary study of fumagillin.J. Chem. Soc. ,11:1473–1474.
Bogdanov, S., Tomislav, J., Robert, S. and Peter, G.(2008). Honey for Nutrition and Health, a Review. American Journal of the College of Nutrition, 27: 677-689.
Colin G Mitchell, Joan Slight and Kenneth Donaldson. (1997). Diffusible component from the spore surface of the fungus Aspergillusfumigatuswhich inhibits the macrophage oxidative burst is distinct from gliotoxin and other hyphal toxins. Thorax,52:796–801.
Crane, E. (1975). History of honey. In Crane E (ed): “Honey, a comprehensive survey.” 6 London: William Heinemann, pp. 439-488.
Crane, E. (1983). The archaeology of beekeeping.” London: Gerald Duckworth and Co. Cornell University Press.
Didier E.S. (2005). Microsporidiosis: an emerging and opportunistic infection in humans and animals, Acta Trop, 94: 61–76.
Diserens, J. M (2007).Contaminants and residues in Food.Strategies to screen and analyse Veterinary drug residues in food from animal origin. 5th International Fresenius conference Nestle Research Center, 1000 Lausanne 26Switzerland.Availablefrom:www.biocop.org/Contaminant_Residues_in_Food_5th_Fresenuisppt.pdf
EMEA (2000).The European Agency for the Evaluation of Medicinal Products, Veterinary Medicines and Information Technology,Committee for Veterinary Medicinal Products.
Emmanouel K., Chrysoula T., Urania M. and Andreas T. (2008).Residue distribution of the acaricidecoumaphos in honey following application of a new slow-release formulation. Pest Management Science, 64(2):165-172.
Evans, E.P., Breckon G. and Ford, C.F. (1964). An air drying method for meiotic preparations from mammalian testes.Cytogenetics.3:289 – 294.
Fardis, M., Pyun, H.J., Tario, J., Jin, H., Kim, C.U. and Ruckman, J. (2003). Design, synthesis and evaluationof a series of novel fumagillin analogues.Bioorg Med Chem, 11: 5051-5058.
Geni da, S. S., Luís Carlos, M., OrgedaLuiza, A., Domingues, Z. V., Franco Nascimento, F., IvaniPozar, O., Augusta Carolina, de C. and Carmello, M.(2007). Determination of chemical elements in africanizedApismellifera (Hymenoptera: Apidae) honey samples from the State of Piauí,.Brazil ,SCIELO.30 no.4 São Paulo.
Golden, M.C., Hahm, S.J., Elessar, R.E., Saksonov, S. and Steinberg , J.J. (1998). DNA damage by gliotoxinfromAspergillusfumigatus.Mycoses. 41: 97-104.
Hanaa, I. Assil and Peter Sporns.( 1991).ELISA and HPLC ethods for analysis of fumagillin and its decomposition products in honey. J. Agric. Food Chem,39 (12): 2206–2213.
Heil, J.,Reifferscheid, G., Waldmann, P., Leyhausen, G. And Geurtsen, W. (1996). Genotoxicity of dental materials.Mutat Res, 368: 181-194.
Heitkamp, K (1984) Pro und kontraHonig - Sind AussagenzurWirkung des Honigs "wissenschaftlichhinreichendgesichert"? SchriftenzurOecotrophologie: 1-60.
Heitkamp K. K. and M M Busch-StockfischZLebensmUntersForsch(1986). The pros and cons of honey--are statements about the effects of honey scientifically verified?,182(4):279-286.
John, M., D. Stanley Tarbel, William E. Lovett, and Alexander D. Cross. (1956). The chemistry of fumagillin. IV. The presence of epoxide grouping and other observations in the nature of oxygen functions, Contributions from the department of chemistry of the University of Rochester.
Kulic, M.,Aleksic,N.,Stanimirovic,Z.,Ristic,S. and Medenica, S. (2009). Examination Of Genotoxic Effects OfFumagillinin vivo.Genetika, 41 (3): 329 -338.
Liu, S.,Widom, J., Kemp, C.W., Crews, C.M. and Clardy, J., (1998). Structure of human methionine aminopeptidase-2 complexed with fumagillin, Science, 282: 1324-1327.
Louveaux,J. (1985). Le Miel, in Cahier NutritioneletDietetique. 20(1):57-70.
Mazzanti, C.M., Tandle, A., Lorang, D., Costouros, N., Roberts, D., Bevilacqua, G., and Steven K. Libutti.(2004). Early genetic mechanisms underlying the inhibitory effects of endostatin and fumagillin on human endothelial cells.Genome Res. 14, 1585-1593.
Molina, J. M., Tourneur, M., Sarfati, C., Chevret, S., de Gouvello, A., Gobert, J. G., Balkan, S. and Derouin, F.(2002). Fumagillin treatment of intestinal microsporidiosis.N. Engl. J. Med. 346:1963-1969.
Morris D.J., Adams A, Smith P, Richards RH.(2003). Effects of oral treatment with TNP-470 on rainbow trout (Oncorhynchusmykiss) infected with Tetracapsuloidesbryosalmonae(Malacosporea), the causative agent of proliferative kidney disease, Aquaculture. 221: 51-64.
Nieminen, S.M., Maki-Paakkanen, J., Hirvonen, M.R., Roponen, M. and Von Wright, A. (2002).Genotoxicityofgliotoxin, a secondary metabolite of Aspergillusfumigatus, in a battery of short-term tests ystems.Mutat Res. 520: 161-170.
Perez, R.A., Iglesias, M.T., Pueyo, E., Gonzalez, M. and de Lorenzo, C. (2007) .Amino acid composition and antioxidant capacity of Spanish honeys. J Agric Food Chem ,55:360-365.
Richard, J.L., Lyon, R.L., Fichtner, R.E. and Ross, P.F.(1989). Use of thin layer chromatography for detection and high performance liquid chromatography for quantitating gliotoxin from rice cultures of Aspergillusfumigatus Fresenius. Mycopathologia, 107:145–151.
Sabater-Vilar, M., Nijmeijer, S. and Fink-Gremmels, J. (2003).Genotoxicity assessment of five tremorgenicmycotoxins (fumitremorgen B, paxilline, penitrem A, verruculogen, and verrucosid) producedby molds isolated from fermented meats. J Food Protect. 66: 2123-2129.
SAS . (2010) . SAS/STAT , Statistical analysis system . SAS Institute Inc ., Cary , NC. USA.
Sin, N., Meng, L., Wang, M.Q.W., Wen, J.J., Bornmann, W.G. and Crews, C.M. (1997). The anti-angiogenic agent fumagillin covalently binds and inhibits the methionine aminopeptidase, MetAP-2. Proc. Nat. Acad. Sci. USA ,94: 6099-6103.
Stanimirovic Z., StevanovicJevrosima, Kulic M. and Stojic V.(2006). Frequency of chromosomal aberrations in the evaluation of genotoxic potential of Dicyclohexylamine (Fumagillin) in vivo.ActaVeterinaria (Beograd), 56 ( 4): 353-366.
Stanimirovic, Z., Pejin I. I., Kuli Z. and Djiporovic M. (2007). Evaluation Of Genotoxic Effects Of Fumagillin By Sister Chromatid Exchange And Chromosomal Aberration Tests In Human Cell Cultures. Actaveterinaria (Beograd), 57 (2-3): 257-273.
Stanimirovic, Z., Stevanovic J., Bajic V. and Radovic I. (2007). Evaluation of genotoxic effects of fumagillin by cytogenetic tests in vivo. Mutat Res., 628(1): 1-10.
Stanimirovic Z, NevenkaAleksic, Kulic M, and Maletic M. (2010).Fumagillin-induced chromosome aberrations in mouse bone –marrow cells. Arch. Biol. Sci., Belgrade, 62 (1): 47-55.
Steel , RGD. andTorrie,JH.(1980). Principles and Procedures of Statistics.2nd Ed.McGraw-Hill Book Com. New York.
StevanovićJ.,StanimirovićZ., Radaković M. and V. Stojić.(2008).In vitro evaluation of the clastogenicity of fumagillin. Environ. Mol. Mutagen., 49: 594-601.
Toxicological Evaluation, last updated .(2000). Dicyclohexylamine, CAS No. 101-83-7. BG Chemie, Berufsgenossenschaft der chemischenIndustrie,No. 212.
Zuzana ,Barboráková , Roman Labuda , Georg Häubl and Dana Tančinová. (2012).Effect of glucose concentration and growth conditions on the fungal biomass, pH of media and production of Fumagillin by a non-pathogenic strain penicilliumscabrosum.Journal of Microbiology, Biotechnology and Food Sciences,1 (4): 466-477.
Birch, A. J. and Hussain S. F. (1969).Studies in relation to biosynthesis.Part 38.A preliminary study of fumagillin.J. Chem. Soc. ,11:1473–1474.
Bogdanov, S., Tomislav, J., Robert, S. and Peter, G.(2008). Honey for Nutrition and Health, a Review. American Journal of the College of Nutrition, 27: 677-689.
Colin G Mitchell, Joan Slight and Kenneth Donaldson. (1997). Diffusible component from the spore surface of the fungus Aspergillusfumigatuswhich inhibits the macrophage oxidative burst is distinct from gliotoxin and other hyphal toxins. Thorax,52:796–801.
Crane, E. (1975). History of honey. In Crane E (ed): “Honey, a comprehensive survey.” 6 London: William Heinemann, pp. 439-488.
Crane, E. (1983). The archaeology of beekeeping.” London: Gerald Duckworth and Co. Cornell University Press.
Didier E.S. (2005). Microsporidiosis: an emerging and opportunistic infection in humans and animals, Acta Trop, 94: 61–76.
Diserens, J. M (2007).Contaminants and residues in Food.Strategies to screen and analyse Veterinary drug residues in food from animal origin. 5th International Fresenius conference Nestle Research Center, 1000 Lausanne 26Switzerland.Availablefrom:www.biocop.org/Contaminant_Residues_in_Food_5th_Fresenuisppt.pdf
EMEA (2000).The European Agency for the Evaluation of Medicinal Products, Veterinary Medicines and Information Technology,Committee for Veterinary Medicinal Products.
Emmanouel K., Chrysoula T., Urania M. and Andreas T. (2008).Residue distribution of the acaricidecoumaphos in honey following application of a new slow-release formulation. Pest Management Science, 64(2):165-172.
Evans, E.P., Breckon G. and Ford, C.F. (1964). An air drying method for meiotic preparations from mammalian testes.Cytogenetics.3:289 – 294.
Fardis, M., Pyun, H.J., Tario, J., Jin, H., Kim, C.U. and Ruckman, J. (2003). Design, synthesis and evaluationof a series of novel fumagillin analogues.Bioorg Med Chem, 11: 5051-5058.
Geni da, S. S., Luís Carlos, M., OrgedaLuiza, A., Domingues, Z. V., Franco Nascimento, F., IvaniPozar, O., Augusta Carolina, de C. and Carmello, M.(2007). Determination of chemical elements in africanizedApismellifera (Hymenoptera: Apidae) honey samples from the State of Piauí,.Brazil ,SCIELO.30 no.4 São Paulo.
Golden, M.C., Hahm, S.J., Elessar, R.E., Saksonov, S. and Steinberg , J.J. (1998). DNA damage by gliotoxinfromAspergillusfumigatus.Mycoses. 41: 97-104.
Hanaa, I. Assil and Peter Sporns.( 1991).ELISA and HPLC ethods for analysis of fumagillin and its decomposition products in honey. J. Agric. Food Chem,39 (12): 2206–2213.
Heil, J.,Reifferscheid, G., Waldmann, P., Leyhausen, G. And Geurtsen, W. (1996). Genotoxicity of dental materials.Mutat Res, 368: 181-194.
Heitkamp, K (1984) Pro und kontraHonig - Sind AussagenzurWirkung des Honigs "wissenschaftlichhinreichendgesichert"? SchriftenzurOecotrophologie: 1-60.
Heitkamp K. K. and M M Busch-StockfischZLebensmUntersForsch(1986). The pros and cons of honey--are statements about the effects of honey scientifically verified?,182(4):279-286.
John, M., D. Stanley Tarbel, William E. Lovett, and Alexander D. Cross. (1956). The chemistry of fumagillin. IV. The presence of epoxide grouping and other observations in the nature of oxygen functions, Contributions from the department of chemistry of the University of Rochester.
Kulic, M.,Aleksic,N.,Stanimirovic,Z.,Ristic,S. and Medenica, S. (2009). Examination Of Genotoxic Effects OfFumagillinin vivo.Genetika, 41 (3): 329 -338.
Liu, S.,Widom, J., Kemp, C.W., Crews, C.M. and Clardy, J., (1998). Structure of human methionine aminopeptidase-2 complexed with fumagillin, Science, 282: 1324-1327.
Louveaux,J. (1985). Le Miel, in Cahier NutritioneletDietetique. 20(1):57-70.
Mazzanti, C.M., Tandle, A., Lorang, D., Costouros, N., Roberts, D., Bevilacqua, G., and Steven K. Libutti.(2004). Early genetic mechanisms underlying the inhibitory effects of endostatin and fumagillin on human endothelial cells.Genome Res. 14, 1585-1593.
Molina, J. M., Tourneur, M., Sarfati, C., Chevret, S., de Gouvello, A., Gobert, J. G., Balkan, S. and Derouin, F.(2002). Fumagillin treatment of intestinal microsporidiosis.N. Engl. J. Med. 346:1963-1969.
Morris D.J., Adams A, Smith P, Richards RH.(2003). Effects of oral treatment with TNP-470 on rainbow trout (Oncorhynchusmykiss) infected with Tetracapsuloidesbryosalmonae(Malacosporea), the causative agent of proliferative kidney disease, Aquaculture. 221: 51-64.
Nieminen, S.M., Maki-Paakkanen, J., Hirvonen, M.R., Roponen, M. and Von Wright, A. (2002).Genotoxicityofgliotoxin, a secondary metabolite of Aspergillusfumigatus, in a battery of short-term tests ystems.Mutat Res. 520: 161-170.
Perez, R.A., Iglesias, M.T., Pueyo, E., Gonzalez, M. and de Lorenzo, C. (2007) .Amino acid composition and antioxidant capacity of Spanish honeys. J Agric Food Chem ,55:360-365.
Richard, J.L., Lyon, R.L., Fichtner, R.E. and Ross, P.F.(1989). Use of thin layer chromatography for detection and high performance liquid chromatography for quantitating gliotoxin from rice cultures of Aspergillusfumigatus Fresenius. Mycopathologia, 107:145–151.
Sabater-Vilar, M., Nijmeijer, S. and Fink-Gremmels, J. (2003).Genotoxicity assessment of five tremorgenicmycotoxins (fumitremorgen B, paxilline, penitrem A, verruculogen, and verrucosid) producedby molds isolated from fermented meats. J Food Protect. 66: 2123-2129.
SAS . (2010) . SAS/STAT , Statistical analysis system . SAS Institute Inc ., Cary , NC. USA.
Sin, N., Meng, L., Wang, M.Q.W., Wen, J.J., Bornmann, W.G. and Crews, C.M. (1997). The anti-angiogenic agent fumagillin covalently binds and inhibits the methionine aminopeptidase, MetAP-2. Proc. Nat. Acad. Sci. USA ,94: 6099-6103.
Stanimirovic Z., StevanovicJevrosima, Kulic M. and Stojic V.(2006). Frequency of chromosomal aberrations in the evaluation of genotoxic potential of Dicyclohexylamine (Fumagillin) in vivo.ActaVeterinaria (Beograd), 56 ( 4): 353-366.
Stanimirovic, Z., Pejin I. I., Kuli Z. and Djiporovic M. (2007). Evaluation Of Genotoxic Effects Of Fumagillin By Sister Chromatid Exchange And Chromosomal Aberration Tests In Human Cell Cultures. Actaveterinaria (Beograd), 57 (2-3): 257-273.
Stanimirovic, Z., Stevanovic J., Bajic V. and Radovic I. (2007). Evaluation of genotoxic effects of fumagillin by cytogenetic tests in vivo. Mutat Res., 628(1): 1-10.
Stanimirovic Z, NevenkaAleksic, Kulic M, and Maletic M. (2010).Fumagillin-induced chromosome aberrations in mouse bone –marrow cells. Arch. Biol. Sci., Belgrade, 62 (1): 47-55.
Steel , RGD. andTorrie,JH.(1980). Principles and Procedures of Statistics.2nd Ed.McGraw-Hill Book Com. New York.
StevanovićJ.,StanimirovićZ., Radaković M. and V. Stojić.(2008).In vitro evaluation of the clastogenicity of fumagillin. Environ. Mol. Mutagen., 49: 594-601.
Toxicological Evaluation, last updated .(2000). Dicyclohexylamine, CAS No. 101-83-7. BG Chemie, Berufsgenossenschaft der chemischenIndustrie,No. 212.
Zuzana ,Barboráková , Roman Labuda , Georg Häubl and Dana Tančinová. (2012).Effect of glucose concentration and growth conditions on the fungal biomass, pH of media and production of Fumagillin by a non-pathogenic strain penicilliumscabrosum.Journal of Microbiology, Biotechnology and Food Sciences,1 (4): 466-477.
Downloads
Published
2013-06-30
How to Cite
Zuhdi, S. S., & AL-Asady, A. A. B. (2013). Cytogenetic Effects of Honey Contaminated With Fumagillin (Dicyclohexylamine) on Male Mice Mus Musculus Balb/C In Vivo. Science Journal of University of Zakho, 1(1), 120–131. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/55
Issue
Section
Science Journal of University of Zakho
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License [CC BY-NC-SA 4.0] that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work, with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online.
