Histological Changes in Some Organs of The Female Rats Infected With Toxoplasma Gondii Parasite Isolated From Embryo of Aborted Sheep

  • Liqaa H. Al-Delami University of Mosul
  • Buthaina H. Al-Sabawi University of Mosul
  • Firas M. Basheer University of Mosul
  • Hafidh I. Al-Sadi University of Mosul
Keywords: Toxoplasma gondii, histopathological changes, Sheep


The current study included identifying the lesions and the histological changes caused by the intracellular parasite Toxoplasma gondii, which was isolated from embryos of aborted ewes. Intraperitonially injected in 15 female albino rats (3 months old) with suspension containing 100 tissue cysts.Histological section showed after four months of injection a chronic infection characterized by autolysis in all sections, where the liver sections showed expansion in the central hepatic veins, congestion in sinusoidal curves with irregularity in hepatic cords, and the presence of parasites in the liver cells. The brain tissue showed vacuoles in the neurons with an increase in the number of Purkenji cells. Kidney sections were characterized by degenerative and necrotic changes in the endothelial cells of the proximal and distal convoluted tubules with Sloughing of necrotic and degenerated cells of the tubes which accumulated inside the cavity. The parasites appeared in the endothelial cells of the glomerular tufts. Ovary and the uterus showed increased vascular wall thickness, furthermore, the spleen showed autolytic changes, deposition of pigment with the presence of parasites within the cells.

Author Biographies

Liqaa H. Al-Delami, University of Mosul

Department of biology, Education College for Girls, University of Mosul, Iraq.

Buthaina H. Al-Sabawi, University of Mosul

Department of anatomy, College of medicine, University of Mosul, Iraq.

Firas M. Basheer, University of Mosul

Department of biology, Education College for Girls, University of Mosul, Iraq.

Hafidh I. Al-Sadi, University of Mosul

Department of pathology, College of Veterinary, University of Mosul, Iraq.


Ahmed, Y.F.; Sokkar, S.M.; Desouky, H.M., and Soror, A.H. (2008). Abortion due to toxoplasmosis in small ruminants. Glob. Veteri., 6:337-342.
Aitken, I.D. (2007). Diseases of sheep. 4th ed. Blackwell, Oxford, pp:13-142.
Barakat, A.M.A.; Abd-Elaziz, M.M., and Fadaly, H.A. (2009). Comparative diagnosis of toxoplasmosis in Egyptian small ruminant by indirect hemagglutination assay and ELISA. Glob. Veteri., 3:9-14.
Buxton, D.; Maley, S.W.; Wright, S.E.; Rodger, S.; Bartley, P.,
and Innes, E.A. (2007). Toxoplasma gondii and Ovine toxoplasmosis: new aspects of an old story, Veteri. Parasitol., 149:25-28.
Coppin, A.; Dzierszinski, F.; Legrand, S.; Mortuaire, M.; Ferguson, D.J.P, and Tomavo, S. (2003). Developmentally regulated biosynthesis of carbohydrate and storage polysaccharide during differentiation and tissue cyst formation in Toxoplasma gondii .Biochimie., 85:353-61.
Djurkovic-Djakovic, O.; Klun, I.; Khan, A.; Nikolic, A.; Kne-zevic, U.S., and Sibley, L.D. (2006). A human origin type II strain of Toxoplasma gondii causing severe encephalitis in mice. Micr and infect.,20:1-7.
Hasan, T.A.; Ayan, N.G.; Slia, C., and Ogur, K. (2013). Hepatic stellate cells increase in Toxoplasma gondii infection in mice. Parasites &vectors, 6:135.
Husein, M.Q., and Kridli, R.T. (2003). Effect of progesterone Prior to GnRH-PGF2alpha treatment on induction of Ostrys and pregnancy in anoestrous Awassi ewes. Reprodu. in Domest. Anim., 38(3):228-232.
Laug, C.; Gross, U., and Luder, C.G. (2006). Subversion of innate and adaptive immuneresponses by Toxoplasma gondii. Parasitol. Res., Oct 6.
Mohamad,A.A.; Mustafa, M.A.; Nektarios, D.G., and Shawkat, Q.L.(2010).Ovine and caprine toxoplasmosis(Toxoplasma gondii). Iranian Journal of Veterinary Science and Technology, 2(2):61-76.
Montoya, J.G., and Liesenfeld, O. (2004). Toxoplasmosis. Lancet ., 363: 1965-76.
Mordue, D.G.;Monroy, F.; Regina, M.L., Dinarello; C.A., and Sibley, L.D. (2001). Acute Toxoplasmosis lead to lethal overproduction of Th1 cytokines. J. Immunol., 45:74-84.
Pearse, A.G.E. (1985). Histochemistry Theoretical and applied, 4 thed, Analytical Technology, Churehiy Livingston, Ediburgh, UK.
Pinheiro, H.W.; Mota, R.A.; Olibeira, A.A.; Faria, E.B.; Gondim, L.F.; da Silva,A.V., and Anderlini, G.A. (2009). Prevalence and risk factors associated to infection by Toxoplasma gondii in ovine in the state of Alagoas, Brazil.Parasitol. Res., 105:709-715.
Ramzam, M;, AKgtar, M.; Muhammad, F.;Hussain, I.; Hiszszynska-Sawicka, E.; Haq, A.U.; Mahmood, M.S., and Hafeez, M.A. (2009). Seroprevelencen of Toxoplasma gondii in sheep and goats in Rahim Yar Khan (Punjab), Pakistan, Tropi.Anim., 41: 1225-1229.
Riyadh, R. (2005). Flock level seroprevalence of and risk factors for Toxoplasma gondii in sheep and goats in northern
Jordan M.Sc. Thesis, Jordan University of Science and Technology, Irbid, Jordan.
Rodger, S.M.; Maley, S.W.; Wright, S.E.; Mackellar, A.; Wesley, F.; Sales, J., and Buxton, D. (2006). Role of endogenous transplacental transmission in toxoplamosis in sheep, Veteri. Reco., 159:768-771.
Savio, E., and Nieto, A. (1995).Ovine toxoplasmosis:
Seroconversion during pregnancy and lamb birth rate in
Uruguaayan sheep flocks Veteri. Nary. parasitol., 60: 241-247.
Sukthana, Y.; Waree, P.; Pongponratn, E.; Chaisri, U., and Riganti, M. (2003). Pathologic study of acute Toxoplasmosis in experimental animals, Trop. Med., 34 (1):16-21.
Williams, R.H.; Hughes, J.M.; Thomasson, D.; Terry, R.S.; Duncanson, P.,;Smith, J.E., and Hide, G. (2008). Evidence that primary infection of Charollais sheep with Toxoplasma gondii may not prevent fetal infection and abortion in subsequent lambings, parasitol., 135: 167-173.
Wong, S., and Remington, J.S. (1994). Toxoplasmosis in pregnancy. Clin. Infect. Dis., 8:853-861.
How to Cite
Al-Delami, L., Al-Sabawi, B., Basheer, F., & Al-Sadi, H. (2014). Histological Changes in Some Organs of The Female Rats Infected With Toxoplasma Gondii Parasite Isolated From Embryo of Aborted Sheep. Science Journal of University of Zakho, 2(2), 276-284. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/223
Science Journal of University of Zakho