Effect of Parainfluenza Virus Infection on Human Immuno-Inflammatory Genes Expression and Gene Ontology Analysis

  • ABDULRHEM THONNON AL-GHAZAL University of Mosul
  • SAID IBRAHEM ISMAIL University of Jordan
  • YOUSUF IBRAHEM UMARY University of Jordan
  • RULA FAIEK AL-KHUZIE University of Jordan
Keywords: RT-PCR-arrays, Parainfluenza virus- white blood cell interaction, inflammatory gene expression, cytokines, cytokines receptors

Abstract

Parainfluenza virus (PIV) causes respiratory infections with high rates of morbidity and mortality in Children and immune-deficiency patients worldwide.  The aim of this study is to detect the changes of mRNA expression of 84 human inflammatory genes and gene ontology analysis. From 90 patients only 2 gave positive IgM –PIV test. PIV infection caused over expression of 25 prominent genes (from 168 genes) affect functional pathways, ten of these genes regard as proinflammatory genes. Three genes were down-regulated; this suggests that the host-viral interaction is a cellular response against viral infection.  In conclusion: Gene ontology analysis revealed that PIV stimulated 4 different key role immunological pathways represented by cytokine-cytokine receptor interaction, NOD-like receptor signaling, Toll-like receptor signaling, and asthma pathway. The patients are mostly infected with related subtypes of PIV. These results may help in further analysis of viral-host interaction.

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Published
2013-06-30
How to Cite
AL-GHAZAL, A., ISMAIL, S., UMARY, Y., & AL-KHUZIE, R. (2013). Effect of Parainfluenza Virus Infection on Human Immuno-Inflammatory Genes Expression and Gene Ontology Analysis. Science Journal of University of Zakho, 1(1), 8-21. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/30
Section
Science Journal of University of Zakho