Screening of Tpo Gene C.1708c<T And C.1978c<G Mutations In Iraqi Patients with Hypothyroidism and Thyroid Cancer
Article Sidebar
-
Hypothyroidism, Thyroid cancer, TPO, c.1708C<T, c.1987C<G, Mutations, LAN primers
Abstract
Thirty seven patients (hypothyroidism, n=20 and thyroid cancer, n=17) and twenty five healthy individuals (control) were used in this study. These patients, who attended the endocrinologist in Nuclear Medicine Hospital and Al Yarmouk Nuclear Medicine Department in Baghdad, were selected during a period from July to October 2009.
Modified specific locked nucleic acid (LNA) primers were used for the detection of TPO gene mutations which include c.1708 C> T and c.1978 C>G. Seven mutations were found in TPO gene. Mutations involved transition cytosine by thymine at position 1708 of the exon 10 (c.1708C<T) were found in four thyroid cancer patients, while, transversion of cytosine by guanine at the position 1978 of exon 11 (c.1978C<G) were found in one thyroid cancer and two hypothyroidism patients. It can be concluded that the c.1708C<T and / or c.1978C<G mutant TPO in hypothyroidism and thyroid cancer patients studied are very likely to be properly expressed but lacks enzymatic activity.
Full text article
Authors
Copyright (c) 2013 Al-Faisal A.H.M., Abdul-Hassan I.A., Al-Ramahi I. J., Barusrux S.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License [CC BY-NC-SA 4.0] that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work, with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online.