Genotoxic and Histopathological Effects of Chlorpyrifos on African Catfish, C. gariepinus and Ameliorative Potentials of Ackee Apple, blighia sapida Seed
Chlorpyrifos (CPF) is a very toxic pesticide commonly used for controlling agriculturally important pests. The present study investigates DNA damaging effects of CPF on Clarias gariepinus was assessed using genetic assays. Fish were exposed to varying concentrations of CPF (100ppm, 200ppm and 300ppm) at 96 hours (acute exposure). LC50 of the pesticide was found to be 120ppm and one-tenth of the LC50 (12ppm) was taken for the chronic exposure. Distilled water and colchicine were used as negative and positive controls respectively. After 28 days of chronic exposure, fish were fed with inclusion of B. sapida seed (powder) for 14 days. Liver and gills of the fish were removed following 96h exposure, days 7, 14 and 28 of the chronic exposure as well as after the period of amelioration (14 days) for CA assay and histopathological analysis. The results of CA assay showed statistically significant (p ˂ 0.05) increase in CA in a dose-dependent manner for all the exposed groups after acute exposure and time dependent after chronic exposure. Also, this study showed that CPF can potentially induce genotoxic and histopathological changes in fish and other aquatic organisms.
Ahmadivand S., Farahmand, H., Mirvaghefi, A.R., Eagderi,S., Shokrpoor, S., and Rahmati-Holasoo, H. (2014): Histopathological and Haematological Response of Male Rainbow Trout (Oncorhynchus mykiss) Subjected to Butachlor. Veterinarni Medicina, 59 (9): 433-439.
Ali, D., Nagpure, N.S., Kumar, S., Kumar, R., Kushwaha, B., and Lakra, W.S. (2009). Assessment of Genotoxic and Mutagenic Effects of Chlorpyrifos in Freshwater Fish Channa punctatus (Bloch) Using Micronucleus Assay and Alkaline Single-Cell gel Electrophoresis. Food Chemistry and Toxicology, 47(3):650-656.
Al-Sabti, K. and Metcalfe, C.D. (1995). Fish Micronuclei for Assessing Genotoxicity in Water: sensitive Monitor for Aquatic Pollution, African Journal of Mutation Research, 343: 121-135.
Ambali, S.F., Abubakar, A.T., Shittu, M., Yaqub L.S., Anafi, S.B. and Abdullahi, A. (2010). Chlorpyrifos induced alteration of hematological parameters in wistar rats: ameliorative effect of zinc. Research Journal of Environmental Toxicology, 4(2):55-66.
Arjmandi, R., Tavakol, M. and Shayeghi, M. (2010). Determination of organophosphorus insecticide residues in the rice paddies. International Journal of Environmental Science Technology, 7 (1): 175-182.
Asano, Y.F.N., Sifuni, T. and Ojima, Y. (1998). Development of genotoxic assay in systems that use aquatic organisms. Mutation Research, 399: 125-133.
Ayoola, S.O. and Ajani E.K. (2008). Histopathological effects of cypermethrin on juvenile african catfish (Clarias gariepinus). World Journal of biological research 001: 2.
Banaee, M., Sureda, A. Mirvaghefi, A.R. and Ahmadi, K. (2011). Effects of Diazinon on Biochemical Parameters of Blood in Rainbow Trout (Onchorhynchos mykiss). Pesticide Biochemistry and Physiology, 99:1-6.
Biswas, S. and Manna, G.K. (1992). The ‘Hay Bacillus’, Bacillus subtillis as Genotoxic Agent in Treated Fresh Water Tilapia, In: Perspectives of Cytology and Genetics Manna G.K. and Roy, S.C. (Ed) 7, pp 945-952.
Bolognesi, C. (2003). Genotoxicity of Pesticides: A Review of Human Biomonitoring Studies. Mutation Research, vol. 543, pp. 251-272.
Bremmer, J.N., Walker, A.I.T. and Grasso, P. (1988). Is Dichlorvos a Carcinogenic Risk for Humans? Mutation Research, 209: 35-44.
Bryant, P. E. (1998).The signal model: a possible explanation for the conversion of DNA double-strand breaks into chromatid breaks. International Journal of Radiation Biology, 73: 243–251.
Candioti, J.V., Soloneski, S., and Larramendy, M.L. (2010). Genotoxic and Cytotoxic Effects of the Formulated Insecticide Aficida on Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces: Poeciliidae). Mutation Research, 703, 180-186.
Caporale L.H. (Ed.), (1999). Molecular Strategies in Biological Evolution. Annal of New York Academic of Science. 870.
Çavas, T., Garanko, N.N. and Arkhipchuk, V.V. (2005). Induction of Micronuclei and Binuclei in Blood, Gill and Liver cells of Fishes Subchronically Exposed to Cadmium Chloride and Copper Sulphate. Food Chemistry and Toxicology, 43:569-574.
Cavas, T., and Ergene-Gözükara S. (2005). Micronucleus Test in Fish Cells: A Bioassay for In situ Monitoring of Genotoxic Pollution in the Marine Environment. Environmental Molecular Mutagen. 46:64-70.
Daoud, A.A., Nagpur, N.S., Sudhir, K.B., Ravindra, K., Kushwahaa, B. and Lakra, W.S., (2009). Assessment of Genotoxic and Mutagenic Effects of Chlorpyrifos in Freshwater Fish Channa punctatus (Bloch) Using Micronucleus Assay and Alkaline Single-cell Gel Electrophoresis. Food and Chemical Toxicology, 47: 650-656.
Dar, S.A., Yousuf, A.R., Balkhi, M.H., Ganai, F.A. and Bhat, F.A. (2015): Assessment of Endosulfan Induced Genotoxicity and Mutagenicity Manifested by Oxidative Stress Pathways in Freshwater Cyprinid Fish Crucian Carp (Carassius carassius). Chemosphere, 120, 273-283.
Dede, E.B. (1992). Effect of Lindane Pretreatment on Diclovors Toxicity in vitro and in vivo. Ph.D Thesis. Ahmadu Bello University, Zaria, Nigeria.
De Silva, P. M. C.S. and Samayawardhena, L. A. (2002). Low concentrations of lorsban in water result in far reaching behavioral and histological effects in early life stages in guppy, Ecotoxicology and Environmental Safety, 53, 248-254.
Devi, Y. and Mishra, A. (2013). Study of behavioural and morphological anomalies of fry fish of freshwater teleost, Channa punctatus under chlorpyrifos intoxication, International Journal of Pharma and Bio Sciences, 4(1B), 865-874.
Ekué, M .R. M., Sinsin, B., Eyog-Matig, O. and Finkeldey, R. (2010). Uses, Traditional Management, Perception of Variation and Preferences in Ackee (Blighia sapida, K.D. Koenig) Fruit Traits in Benin: Implications for Domestication and Conservation. Journal of Ethnobiology and Ethnomedicine, 6(12), 1-14.
Elezaby, M.M., El-Serafy, S., Heckmann, R.K., Sharf, E., and Seddek M.M. (2001). Effects of Some Toxicants on the Freshwater Fish, Oreochromis niloticus. Journal of Egyptian and German Society of Zoology. 36:407-434.
Elif, I. C. and Erhan, U. (2006). Sub-lethal Effects of Commercial Deltamethrin on the Structure of the Gill, Liver and Gut Tissues of Mosquitofish, Gambusia affinis: A Microscopic Study. Environmental Toxicology and Pharmacology 2, 246–253.
Emmanuel, C.O., Helmina O.A., and Egwim C.E. (2014). Phytochemical Constituents of Seeds of Ripe and Unripe Blighia Sapida (K. Koenig) and Phytochemical Properties of the Seed Oil. International Journal of Pharmaceutical Science Invention. Vol.3, 9, PP. 31-40.
Ensminger, M., Bergin R., Spurlock F., and Goh, K.S. (2011). Pesticide Concentrations in Water and Sediment and Associated Invertebrate Toxicity in Del Puerto and Orestimba Creeks, California, 2007–2008. Environmental Monitoring and Assessment.
EPA (2001). Pesticide IndustrySales and Usages: 1996 and 1997 Market Estimates. www.epa.gov/Obeas1/pestsales/97pestsales/table1.html.
Fulton, M.H. and Key, P.B. (2001). Acetylcholinesterase Inhibition in Estuarine Fish and Invertebrates as an Indicator of Organophosphorus Insecticide Exposure and Effects. Environmental Toxicology and Chemistry, 20: 37-45.
Garcia, C. Z and Martinez, C. B. R. (2012). Biochemical and genetic alterations in the freshwater neotropical fish Prochilodus lineatusafter acute exposure to Microcystis aeruginosa. Neotropical ichthyology. vol.10 no.3:613-622
Giesy, J.P., Solomon, K.R., Coates, J.R., Dixon, K.R., Giddings, J.M. and Kenaga, E.E. (1999). Chlorpyrifos: Ecological Risk Assessment in North American Aquatic Environments. Rev Environmental Contamination Toxicology, 60:1–129.
Hartwell LH, Hood L, Goldberg ML, Reynolds AE, Silver LM, and Veres, R.C. (2000). Genetics: From Genes to Genomes. McGraw Hill Higher Education. ISBN 0-07-540923-2. pp. 70-98.
Hellar, H. (2002). Pesticides Residues in Sugarcane Plantations and Environs after Long Term Use; the Case of TPC Ltd, Kilimanjaro Region, Tanzania.
Humphrey, C.A., Klump, D.W., and Raethke, N. (2004). Ambon Dansel (Pomancentrus amboinensis) as a Bioindicator Organism for the Great Barrier Reef: Responses to Chlorpyrifos. Bulletin of Environmental Contamination and Toxicology. 72, pp 888-895.
John, P.J. (2007). Alteration of Certain Blood Parameters of Freshwater Teleost Mystus Vittatus after Chronic Exposure to Metasystox and Sevin. Fish Physiology and Biochemistry, 33:15-20.
Kamal, K. S. and Renu, C. (2010). Study of Chromosomal Abnormalities in Channa punctatus Exposed to Fenvalerate. Journal of Applied and Natural Science 2 (1): 70-73.
Mohanty, G., Mohanty, J., Nayak, A.K., Mohanty, S. and Dutta, S.K. (2011). Application of Comet Assay in the Study of DNA Damage and Recovery in Rohu (Labeo rohita) Fingerlings after an Exposure to Phorate, an Organophosphate Pesticide. Ecotoxicology, 20, 283-292.
Myers, M.S., Olson, O.P., Johnson, L.L., Stehr, C.S., Homt, R., and Varansi, U. (1992). Hepatic Neoplasm and Related Lesions and Exposure to Toxic Chemicals in Marine Fish from U.S West. Environmental Health Perspective, 90:7-15.
Nagpure, N.S. and Barat, A. (1997). A Simplified Method of Fish Chromosome Preparation by In Vitro colchicine Treatment. Indian Journal of Experimental Biology, 35:915-916.
Nwani C.D., NjokuIvoke, D.O., Ugwu C.A., Onyishi, G.C., Chinedu, P. E. and Ogbonna, S.A. (2013a). Investigation on Acute Toxicity and Behavioral Changes in a Freshwater African Catfish, C. gariepinus (Burchell, 1822) Exposed to Organophosphorous Pesticide, Termifos®. Pakistani Journal of Zoology, vol. 45(4), pp. 959-965.
Nwani, C.D., Ugwu D.O., Okeke, O.C., Onyishi, G.C., Ekeh, F.N., Atama, C, and Eneje, L.O. (2013b). Toxicity of the Clorpyrifos-based Pesticide Termifos: Effects on Behaviour and Biochemical and Haematological Parameters of African catfish Clarias gariepinus. African Journal of Aquatic Science.
Nwani, .CD., Lakra, W.S., Nagpure, N.S., Kumar, R., Kushwaha, B., and Srivastava, S.K. (2010a). Mutagenic and Genotoxic Effects of Carbosulphan in Freshwater Fish Channa punctatus (Bloch) Using Micronucleus Assay and Alkaline Single-Cell Gel Electrophoresis. Food and Chemical Toxicology, 48:202-208.
Nwani, C.D, Nagpure, N.S., Kumar, R, Kushwaha, B., Kumar, P., and Lakra, W.S. (2010b). Lethal Concentration and Toxicity Stress of Carbosulfan, Glyphosate and Atrazine to Freshwater Air Breathing Fish Channa punctatus (Bloch). International Aquatic Research, 2: 105-111.
Okwu, D.E. (2004). Phytochemicals and Vitamin Content of Indigenous Species of South-eastern Nigeria. Journal of Sustain Agriculture Environment, 6: 30-34.
Okwu, D.E. (2005). Phytochemicals, Vitamins and Mineral contents of Two Nigerian Medicinal Plants. International Journal of molecular medicinal and advanced science, 1(4): 375-381.
Osman, A.G., Abd El Reheema, A.M., Moustafa, M.A., Mahmoud, U.M., Abuel-Fadl, K.Y. and Kloas, W. (2011). In Situ Evaluation of the Genotoxic Potential of the River Nile: Micronucleus and Nuclear Lesion Tests of Erythrocytes of Oreochromis niloticus (Linnaeus, 1758) and C. gariepinus (Burchell, 1822). Toxicological and Environmental Chemistry, 93, 1002-1017.
Pan, G. and Dutta, H.M. (1998). The Inhibition of Brain Acetylcholinesterase Activity of Juvenile Largemouth Bass, Micropterus salmoides by Sub-letha Concentrations of Diazinon. Env. Res. Sec A, 79:133-137.
Piemental, D. and Levitan L. (1986). Pesticides- Amounts Applied and Amounts Reaching Pests. Biosciences, 36:86-91.
Ramesh, H. and Muniswamy, D. (2009). Behavioural Responses of the Freshwater Fish, Cyprinus carpio (Linnaeus) Following Sublethal Exposure to Chlorpyrifos; Turkish Journal of Fisheries and Aquatic Sciences 9: 233-238.
Rao, J.V., Begum, G., Pallela, R., Usman, P.K., and Rao, R.N. (2003). Changes in Behavior and Brain Acetylcholinesterase Activity in Mosquito Fish, Gambusia affinis in Rresponse to the Sub-lethal Exposure to Organophosphate Pesticide. International Journal of Environmental Research and Public Health, 2:478–83.
Rao, J.V. (2008). Brain Acetylcholinesterase Activity as a Potential Biomarker for the Aphida Assessment of Organophosphate Pesticide Toxicity in a Euryhaline Fish, Oreochromis mosambicus. Environmental Journal of Bioindicators, 3: 11-22.
Rishi, K.K. and Grewal, S. (1995). Chromosome Aberration Test For The Insecticide, Dichlorvos, of Fish Chromosomes. Mutation Research, 344: 1-4.
Rishi, K.K. and Sunita G. (1993). Cytogenetic Screening of Effect of a Mosquito Larvicide on Kidney Cell Chromosomes of Channa punctatus, Abstr in Eighth India Congress of Cytology and Genetics, pp 87.
Rishi, K.K., and Haobam, M.S. (1990). A Chromosomal Study on Four Species of Snake Heads (Ophicoephalidae, Pisces) With Comments on Their Karyotype Evolution. Caryologia, 43: 163-163.
Rishi, K.K. (1989). Current Status of Fish Cytogenetics. Fish Genetics in India (Das and Jhingran, Eds) Hindasia, New Delhi, pp 1-20.
Siang, H.Y. Yee, L.M. and Seng, C.T. (2007). Acute toxicity of Organochlorine Insecticide Endosulfan and Its Effect on Behaviour and Some Hematological Parameters of Asian Swamp Eel (Monopterus albus). Pesticide Biochemistry and Physiology, 89: 46–53.
Somdare, P.O., Nwani, C.D., Nwadinigwe, A.O., Nwani, J.C., Odo, G.E.,Ugbor, O.N., Ukonze, J.A. and Ezeibe, A.B.C.(2015). Fenthion Induced Toxicity and Histopathological Changes in Gill Tissue of Freshwater African Catfish, C. gariepinus (Burchell, 1822). African Journal of Biotechnology, vol. 14(25), pp. 2103-2113.
Sunmonu, T.O., and Oloyede, O.B.(2006). Changes in Liver Enzyme Activities in African Catfish (Clarias gariepinus) Exposed to Crude Oil. Asian Fisheries Science. 19: 104-109.
Sunanda, M. Rao C. S. J., Neelima, P. Rao, G. K. and Simhachalam, G. (2016). Effects of Chlorpyrifos (an Organophosphate Pesticide) in Fish. Int. J. Pharm. Sci. Rev. Res., 39(1), 299-305
Testai, E., Buratti, F.M., and Consiglio, E.D. (2010). Chlorpyrifos. In: Krieger, R.I., Doull J, van Hemmen, J.J., Hodgson, E., Maibach, H.I., Ritter, L., Ross, J., Slikker, W. (eds) Handbook of Pesticide Toxicology, vol 2. Elsevier, Burlington, MA, pp 1505-1526.
Timchalk, C. (2010). Organophosphorus Insecticide Pharmacokinetics. In: Krieger, R.I., Doull J, van Hemmen, J.J., Hodgson, E., Maibach, H.I., Ritter, L., Ross, J., Slikker, W. (eds) Handbook of Pesticide Toxicology, vol 2. Elsevier, Burlington, MA, pp 1409–1433.
Toni, P. G. and Lília, M. M. (2009). Evaluation of Genotoxicity Using the Micronucleus Assay and Nuclear Abnormalities in the Rropical Sea Fish Bathygobius soporator (Valenciennes, 1837) (Teleostei, Gobiidae). Genetics and Molecular Biology, 32: 394-398.
Topal A. Atamanalp M. Oruç E. Demir Y. Beydemir Ş. and Işık A. (2014). In vivo changes in carbonic anhydrase activity and histopathology of gill and liver tissues after acute exposure to chlorpyrifos in rainbow trout, Arh Hig Rada Toksikol, 65, 377-385.
Veronica, M. Dosso., Jacob K. A., Sussana, C., and Sarah, A. (2014). Ackee (Blighia sapida) fruit arils: Nutritional, Phytochemicals and Antioxidant Properties. International Journal of Nutrition and Food Sciences, 3(6): 534-537.Veterinarni Medicina, 59, 2014 (9): 433-439.
Vocke, G. (1986). The Green Revolution for Wheat in Developing Countries, US Department of Agriculture, USA.
Werimo, K. Bergwerff, A.A. and Seinen, W. (2009). Residue Levels of Organochlorines and Organophosphates in Water, Fish and Sediments from Lake Victoria-Kenyan Portion. Aq. Eco.Health Man., 12:337-4341.
Whitney, K.D., Seidler, F.J., and Slotkin, T.A. (1995). Developmental Neurotoxicity of Chlorpyrifos: Cellular Mechanisms. Toxicology and Applied Pharmacology, 134: 53–62.
Yadav K. K. and Trivedi, S. P. (2009). Chromosomal Chromosomal Aberrations in a Fish, Channa punctata After In vivo Exposure to Three Heavy Metals. Mutation Research, 678:7-12.
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