Characterization of Two Domestic Goat Breeds in Duhok Province / Iraq Using Microsatellites
Keywords:
Duhok, microsatellites, Merzi and Native goatsAbstract
In this study, a molecular‒based technique was employed to characterize two Iraqi goat breeds. For this, thirty blood samples were obtained from two Iraqi indigenous goat breeds (Native and Merzi goat) in Duhok province. Genomic DNA was extracted, out of 8 microsatellites, 6 microsatellites were amplified and produced bands in polymerase chain reactions (PCR). PCR products were subjected to 1.5% agarose gel electrophoresis for fast and easy detection of successful amplification. Then, the PCR products were subjected to 6% Polyacrylamide electrophoresis and stained with silver nitrate. Many distinct alleles were observed that they can be indicative of possible breed marker.Close genetic distance was observed between Native and Merzigoat (D=0.1667). These results can be used to establish national conservation and breed improvement strategies.
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Beckman, J.S., and Weber, J. L.(1992).Survey of Human and Rat Microsatellites. Genomics,12:627–631.
Koreth, J.; O'Leary, J. J., and McGee, O.'D. J.(1996). Microsatellites and PCR Genomic Analysis. J Pathol.,178:239–248.
FAO.(2000). World Watch List for Domestic Animals Diversity.
Iniguez, L. (2005). Characterization of Small Ruminants Breeds in West Asia and North Africa.Vol.1: West Asia. ICARDA.
Jouquand, S.; PriatHitte, C.; Lachaume, P.; Andre, C., and Galibert, F. (2000) . Identification and
characterization of a set of 100 tri- and dinucleotide microsatellites in the canine genome.
Anim. Genet., 31: 266-272.
Kumar, S.; Gupta, T.; Kumar, N.; Dikshit, K.; Navani N.; Jain, P., and Nagarajan, M .(2006) . Genetic variation and relationships among eight Indian riverine buffalo breeds. Mol. Ecol. 15: 593- 600.
Mahmoudi, B.; Bayat,M.; Sadeghi, R.; Babayer, M., and Abdollahi, H.( 2010). Genetic
Diversity among Three Goat Populations Assessed by Microsatellite DNA Markers in Iran. Global Veterinaria, 4 (2): 118-124.
Mason, L.L. (1981). Breeds. Pages.57-110 in Goat Production (C. Gall.ed). Academic Press, London, England.
Michelmore, R. W.; Paran, I., and Kesseli, R. V .(1991). Identification of markers linked to
disease-resistance genes by bulked segregate analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Pro. Nati. Acad. Sci. USA, 88: 9828-9823.
Ministry of Planning .(1991). Means and Prospects of Sheep and Goats Department in Iraq. Directorate of Agricultural Statistics. Central Statistical Bureau. Baghdad. Iraq. In Arabic.
Moioli, B.; Georgoudis, A.; Napolitano, F.; Catillo, G.; Giubilei, E.; Ligda, C.H., and Hassanane M. (2001) . Genetic diversity between Italian, Greck and Egyptian buffalo population Livestock.Pro.Sci., 70:203-211.
Muema, E. K.; Wakhungu, J.W.; Hanotte, O., and Jianlin, H. (2009). Genetic diversity and Relationships of indigenous goats of Sub-Saharan Africa using microsatellite DNA
Marker Livestock Research for Rural Development 21(2) 2009.
Nei, M. (1972). Genetic Distance betweenPopulations. American Naturalist 106:283-292.
Sanguinetti, J.; Neto, E. D., and Simpson, A.J. (1994). Rapid Silver Staining and Recovery of PCR Products Separated on Polyacrylamide Gels. Biotechniques. 17:915-918.
Taha, S. A.(1990). The Effect of Castration and Fattening Period on Quantitative and Qualitative Aspects of Meat Production in Local Goats (PhD Thesis). College of Agriculture. University of Baghdad.In Arabic.
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Published
2013-06-30
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Mohammed, A. B. (2013). Characterization of Two Domestic Goat Breeds in Duhok Province / Iraq Using Microsatellites. Science Journal of University of Zakho, 1(1), 101–107. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/50
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