Detection of Enterohemorrhagic Escherichia Coli O157 in Sheep and Goats Using Fluorogenic and Chromogenic Culture Media
Keywords:
E .coli O157, Sheep, Goats, Feces, MilkAbstract
This study was carried out for the first time to investigate the occurrence of E. coli O157 in sheep and goats in Duhok province, Iraq. A total of 320 samples were collected from April to July 2009 as following: 100 fecal samples from sheep, 100 fecal samples from goats, 60 samples from sheep milk and 60 samples from goat milk. E coli O157 was isolated from 3 (3%) sheep fecal samples and 2 (2%) from goat fecal samples, while no E. coli O157 was detected in the milk samples from sheep and goats. E. coli O157 was detected only among E. coli isolates which were sorbitol non fermentative but MUG positive, while no E. coli O157 was detected among E. coli isolates which were negative for both sorbitol and MUG. All E. coli O157 isolates were resistant to 6 among 12 antimicrobial agents used in vitro drug sensitivity test
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Adwan M.A. and Adwan K.M.(2004). Isolation of shiga toxin Escherichia coli from raw beef in Palestine. International Journal of Food Microbiology, 97, 81-84.
Bauer A.W., Kirby W.M.M., Sherris J.C. and Turck M.(1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45, 493-496.
Bielaszewska M., Janda J., Blahova K., Minarikova H., Jilkova E., Karmali M.A., Laubova J., Sikolova J., Preston M.A., Khakhria R., Karch H., Klazarova H.and Nyc O.(1997). Human Escherichia coli O157:H7 infection associated with the consumption of unpasteurized goat's milk. Epidemiology and Infection, 199,299-305.
Chiueh L.C., Liu F.M.and Shihe D.Y.C.(2002). Prevalence of Shiga toxin-producing Escherichia coli in feces and raw milk of domestic cattle and sheep. Journal of Food Drug and Analysis, 10, 39-46.
Kuntz, T.B.and Kuntz, S.T.(1999). Enterohemorrhagic E. coli infection. Prime Care Updayes Ob/ Gyns, 6(6, 192-196.
Jenkins, C., Pearce, M.C., Chart, H., Cheasty. T., Willshaw, G.A., Gunn, G.J., Doygan, G., Smith, H.R., Synge, B.A. and Frankel, G.(2002). An eight-month study of a population of verocytotoxigenic Escherichia coli (VTEC) in a Scottish cattle herd. Journal of Applied Microbiology, 93,944-953.
Mc Donough P.L., Rossiter C.A., Rebhun R.B., Stehman S.M., Lein D.H.and Shin S.J.(2000). Prevalence of Escherichia coli O157:H7 from dairy cows in New York State and comparison of culture methods used during preharvest food safety investigations. Journal of Clinical Microbiology, 38(1), 318-322.
Molina P.M., Parma A.E.and Sanz M.E.(2003). Survival in acidic and alcoholic medium of shiga toxin-producing Escherichia coli O157:H7 and non-O157:H7 isolates in Argentina. BMC Microbiology, 13, 3-17.
Nataro, J.P. and Kaper, J.B.(1998). Diarrheagenic Escherichia coli. Clinical Microbiology Review, 11, 142-201.
Novotna R., Alexa P., Hamrik J., Madanat A., Smola J.and Cizek A.(2005). Isolation and characterization of Shiga toxin-producing Escherichia coli from sheep and goats in Jordan with evidence of multiresistant serotype O157:H7. Veterinary Medicine –Czech, 50 (3), 111-118.
Ojeda, A., Prado, V., Martinez, J., Arellano, K., Borczyk, A., Johnson, W., Lior, H. and Levine, M.M.(1995). Sorbitol-negative phenotype Enterohemorrhagic Escherichia coli strains of different serotypes and from different sources. Journal of Clinical Microbiology, 33, 2199-2201.
Oppeggaard H., Steinum T.M.and Wasteson Y. (2001). Horizontal transfer of a multi-drug resistance plasmid between coliform bacteria of human and bovine origin in a farm environment. Applied and Environmental Microbiology, 67, 3732-3734
Rubini S., Cardeti G., Amiti S., Manna G., Onorati R., Caprioli A.and Morabito S.(1999). Verotoxin-producing Escherichia coli O157 in sheep milk. Veterinary Record, 144,56.
Shiomi M., Togowa M., Fujita K.and Murata R.(1999). Effect of early oral fluoroquinolones in hemorrhagic colitis due to Escherichia coli O157:H7. Pediatric International: Official Journal of the Japan Pediatric Society, 41, 228-232.
Verma J.C. (1988). Studies on drug resistance in Salmonella organisms from animal sources. Ph.D. Thesis, Bacteriology, Rohilkhand University, Bareilly, India.
Ware, J.M., Abbott, S.L. and Janda, J.M. (2000). A new diagnostic problem: isolation of Escherichia coli O157:H7 strains with aberrant biochemical properties. Diagnostic Microbiology and Infectious Diseases,38, 185-187.
Wong C.S., Jelacic S., Habeeb R.L., Watkins S.L. and Tarr P.I.(2000). The risk of hemolytic uremic syndrome after antibiotic treatment of Escherichia coli O157:H7 infections. New England Journal of Medicine ,342,1930-1936.
Zhang X.P., McDaniel A.D., Wolf L.E., Keusch G.T., Waldor M.K. and Acheson D.W.K.(2000). Quinolone antibiotics induce shiga toxin-encoding bacteriophages, toxin production, and death in mice. Journal of Infectious diseases,181, 664-670.
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Published
2013-06-30
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SAEED, A. Y., & IBRAHIM, K. S. (2013). Detection of Enterohemorrhagic Escherichia Coli O157 in Sheep and Goats Using Fluorogenic and Chromogenic Culture Media. Science Journal of University of Zakho, 1(1), 115–119. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/53
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