Rapid Differentiation of Pleurotus Ostreatus from Pleurotus Sapidus Using Pcr Technique

Authors

  • HADEEL WALEED ABDULMALK University of Baghdad

Keywords:

Pleurotus ostereatus, Pleurotus sapidus, Higher Fungi, PCR, (ITS) region primers, RAPD-PCR, Edible mushrooms

Abstract

Pleurotus ostreatus and Pleurotus sapidus are difficult to differentiate using standard morphologically based characteristics. This study, using two taxon-selective primers for the internal transcribed spacer (ITS) region in the nuclear ribosomal repeat unit. These primers, ITS1-F & ITS4, were intended to be specific for the higher fungi, respectively. RAPD-PCR technique was used to reveal DNA polymorphism in DNA of the two fungi Pleurotus ostreatus and Pleurotus sapidus in order to search for the sources of differences that could be used as a DNA marker represent the differentiation between these  two species . In RAPD-PCR, eight different decamer primers chosen randomly were employed to detect the genetic polymorphisms among studied strains .

Author Biography

HADEEL WALEED ABDULMALK, University of Baghdad

Dept. of Biotechnology, College of Science, University of Baghdad

References

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Published

2013-06-30

How to Cite

ABDULMALK, H. W. (2013). Rapid Differentiation of Pleurotus Ostreatus from Pleurotus Sapidus Using Pcr Technique. Science Journal of University of Zakho, 1(1), 153–157. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/58

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Science Journal of University of Zakho