Rapid Differentiation of Pleurotus Ostreatus from Pleurotus Sapidus Using Pcr Technique
Keywords:
Pleurotus ostereatus, Pleurotus sapidus, Higher Fungi, PCR, (ITS) region primers, RAPD-PCR, Edible mushroomsAbstract
Pleurotus ostreatus and Pleurotus sapidus are difficult to differentiate using standard morphologically based characteristics. This study, using two taxon-selective primers for the internal transcribed spacer (ITS) region in the nuclear ribosomal repeat unit. These primers, ITS1-F & ITS4, were intended to be specific for the higher fungi, respectively. RAPD-PCR technique was used to reveal DNA polymorphism in DNA of the two fungi Pleurotus ostreatus and Pleurotus sapidus in order to search for the sources of differences that could be used as a DNA marker represent the differentiation between these two species . In RAPD-PCR, eight different decamer primers chosen randomly were employed to detect the genetic polymorphisms among studied strains .
References
Albores, S., Pianzzola, M.J. ,Soubes, M., Cerdeiras, M.P. . 2006 . Biodegradation of agroindustrial wastes by Pleurotus spp for its use as ruminant feed . Electric Journal of Biotechnology. Vol. 9 , No. 3 .
Ali, B.A. (2003). Detection of DNA alteration in abnormal phenotype of broiler chicken male by random amplified polymorphic DNA (RAPD). African journal of Biotechnology. 2(6): 153-156.
Baldwin, B.G. (1992). "Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example from the Compositaogy". Molecular Phylogenetics and Evolution 1 (1): 3–16.
Bresinsky, A., O. Hilber, and H. P. Molitoris. 1977. The genus Pleurotus as an aid for understanding the concept of species in Basidiomycetes. Bibl. Mycol. 61:229-258.
Chang, Shu-ting; Miles, Philip G. (2004)."Pleurotus – A Mushroom of Broad Adaptability". Mushrooms: cultivation, nutritional value, medicinal effect, and environmental impact (2nd ed.). CRC Press. pp. 315–325. ISBN 978-0-8493-1043-0.
Firenzuoli F., Gori L., Lombardo G. (2007). The medicinal mushroom Agaricus blazei Murrill: review of literature and pharmaco- toxicological problems. Advance Access Publication, 5 (1): 3-15.
Gardes, M., and Bruns, T.D. (1993). "ITS primers with enhanced specificity for basidiomycetes: application to the identification of mycorrhiza and rusts". Molecular Ecology. 2 (2): 113–118 .
Kernodle, S.P.; Cannon, R.E. and Scandalios, J.G. (1993). Concentration of primer and template qualitatively affects product in RAPD-PCR. Biotechniques. 1: 362-364.
McPherson, M.J. and MØller, S.G. (2001). PCR The basic background to bench. Cornwell Press, Trowbridge, UK.
Moncalvo J., Drehmel D., Vilgalys R. (2000). Variation in modes and rates of evolution in nuclear and mitochondrial ribosomal DNA in the mushroom genus Amanita (Agaricales, Basidiomycota):phylogenetic implications. Mol. Phylogenet. Evol., 16: 8-63.
Pacioni G., Leonardi M., Aimola P., Ragnelli A., Rubini A., Paolocci F.(2007). Isolation and characterization of some mycelia inhabiting Tuber ascomata. Mycol. Res., III: 1450-1460.
Petersen, R. H., and Hughes K. W. . 1999. Species and speciation in mushrooms? Development of a species concept poses difficulties. Bio. Sci. 49:440-452.
Ro H., Kim S., Ryu J., Jeon C., Leed T., Le H. (2007). Comparative studies on the diversity of the edible mushroom Pleurotus eryngii: ITS sequence analysis, RAPD fingerprinting, and physiological characteristics. Mycol. Res. III, 710-715.
Stamets, Paul (2000). "Chapter 21: Growth Parameters for Gourmet and Medicinal Mushroom Species". Growing gourmet and medicinal mushrooms = [Shokuyo oyobi yakuyo kinoko no sabai] (3rd ed.). Berkeley, California, USA: Ten Speed Press. pp. 316–320. ISBN 978-1-58008-175-7.
Williams, J.G.K.; Kubelik, A.R.; Livak, K.J.; Rafalski, J. A. and Tingey, S. V.(1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research.18(22): 6531-6535.
Ali, B.A. (2003). Detection of DNA alteration in abnormal phenotype of broiler chicken male by random amplified polymorphic DNA (RAPD). African journal of Biotechnology. 2(6): 153-156.
Baldwin, B.G. (1992). "Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example from the Compositaogy". Molecular Phylogenetics and Evolution 1 (1): 3–16.
Bresinsky, A., O. Hilber, and H. P. Molitoris. 1977. The genus Pleurotus as an aid for understanding the concept of species in Basidiomycetes. Bibl. Mycol. 61:229-258.
Chang, Shu-ting; Miles, Philip G. (2004)."Pleurotus – A Mushroom of Broad Adaptability". Mushrooms: cultivation, nutritional value, medicinal effect, and environmental impact (2nd ed.). CRC Press. pp. 315–325. ISBN 978-0-8493-1043-0.
Firenzuoli F., Gori L., Lombardo G. (2007). The medicinal mushroom Agaricus blazei Murrill: review of literature and pharmaco- toxicological problems. Advance Access Publication, 5 (1): 3-15.
Gardes, M., and Bruns, T.D. (1993). "ITS primers with enhanced specificity for basidiomycetes: application to the identification of mycorrhiza and rusts". Molecular Ecology. 2 (2): 113–118 .
Kernodle, S.P.; Cannon, R.E. and Scandalios, J.G. (1993). Concentration of primer and template qualitatively affects product in RAPD-PCR. Biotechniques. 1: 362-364.
McPherson, M.J. and MØller, S.G. (2001). PCR The basic background to bench. Cornwell Press, Trowbridge, UK.
Moncalvo J., Drehmel D., Vilgalys R. (2000). Variation in modes and rates of evolution in nuclear and mitochondrial ribosomal DNA in the mushroom genus Amanita (Agaricales, Basidiomycota):phylogenetic implications. Mol. Phylogenet. Evol., 16: 8-63.
Pacioni G., Leonardi M., Aimola P., Ragnelli A., Rubini A., Paolocci F.(2007). Isolation and characterization of some mycelia inhabiting Tuber ascomata. Mycol. Res., III: 1450-1460.
Petersen, R. H., and Hughes K. W. . 1999. Species and speciation in mushrooms? Development of a species concept poses difficulties. Bio. Sci. 49:440-452.
Ro H., Kim S., Ryu J., Jeon C., Leed T., Le H. (2007). Comparative studies on the diversity of the edible mushroom Pleurotus eryngii: ITS sequence analysis, RAPD fingerprinting, and physiological characteristics. Mycol. Res. III, 710-715.
Stamets, Paul (2000). "Chapter 21: Growth Parameters for Gourmet and Medicinal Mushroom Species". Growing gourmet and medicinal mushrooms = [Shokuyo oyobi yakuyo kinoko no sabai] (3rd ed.). Berkeley, California, USA: Ten Speed Press. pp. 316–320. ISBN 978-1-58008-175-7.
Williams, J.G.K.; Kubelik, A.R.; Livak, K.J.; Rafalski, J. A. and Tingey, S. V.(1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research.18(22): 6531-6535.
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Published
2013-06-30
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ABDULMALK, H. W. (2013). Rapid Differentiation of Pleurotus Ostreatus from Pleurotus Sapidus Using Pcr Technique. Science Journal of University of Zakho, 1(1), 153–157. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/58
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