Flow Injection Spectrophotometric Determination of Histamine in Fish Meals
Article Sidebar
-
Flow- Injection, Determination, Histamine, Fish
Abstract
The study describes flow injection spectrophotometric methods for the determination of histamine in fish and fish meal samples. The methods were based on diazotization of p-toluidine and p-nitroaniline and subsequent coupling with histamine in alkaline medium. The calibration graph with diazotizated p-toluidine gives a linear range from 3.0 to 25.0 µg/ml with correlation coefficient 0.9967, while diazotization of p-nitroaniline and coupling with histamine gives a linear range from 0.5 to 22.0µg/ml with correlation coefficient 0.9985and detection limits 1 µg/ml and 0.3 µg/ml respectively. The effect of common amino acids and some ions on the determination of histamine was examined. Amberlite resin (weak cation exchanger) was used for the removing of histamine from interferences. The recommended FI procedures were successfully applied to the quantitation of histamine in 5 commercial fish meal products after appropriate sample treatment.
Full text article
Authors
Copyright (c) 2013 Mohammad S. Abdullah, Nabil A. Fakhri
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License [CC BY-NC-SA 4.0] that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work, with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online.