The Screening Azotobacter Spp., Bioavailability from Four Ecological Systems in Zakho Town, Kurdistam Region – Iraq
DOI:
https://doi.org/10.25271/sjuoz.2022.10.4.951Keywords:
Soil, soil texture, soil bacteria, Azotobacter, legume, pH, bacterial density, Zakho, Kurdistan-Region, IraqAbstract
This project was designed to analyse the impact of several environmental factors on the Azotobacter abundance in soil samples. Azotobacter spp. were isolated from four ecological systems and screened for calculating CFU numbers per two grams of soil. Soil samples with a range of textures were collected from Zakho town (Kurdistan Region – Iraq). Soil particle sizes distributions were measured as (% sand, % silt and % clay). Soil chemical and physical features like (pH, electrical conductivity, organic matters availability) in addition to various soil textures; Loam (4 samples), clay (3 samples), silt (1 sample), clay loam (4 samples) were determined. Electrical conductivity measurements were fallen between 35.7 dSm-1 in sample (L2) and 5.17 dSm-1 in sample (C2). Organic matters availability was from 17.6% in sample (C2) to 4.18% as in sample (R3). Soil samples' pH values were between 9.41 as in sample (R2) and 7.72 that found in sample (L3). Concerning all growth impact factors, the maximum number (370 CFU) of Azotobacter was observed in the R1 sample. No Azotobacter growth was observed in samples; G1 and G2 while sample G3 produced (55 CFU). All isolates show stable growth from the legume rhizosphere zoon (L1 105-, L2 60-, and L3 125-CFU). No isolates were obtained from the R2 soil sample while only 70 CFU were seen in the R3 sample. Samples; C1 and C3 formed 94 and 108 CFU respectively whereas no isolated colonies were noticed in the C2 soil sample.
References
Aasfar A., Bargaz A., Yaakoubi K., et al., (2021): Nitrogen fixing Azotobacter species as potential soil biological enhancers for crop nutrition and yield stability. Front. Microbiol., 12: 628379.
Adviento-Borbe M., Doran J., Drijber R., and Dobermann A., (2010): Soil electrical conductivity and water content after nitrous oxide and carbondioxide emission in intensively managed soils. J. Env. Qua., 2(3): 1999-2010.
Ali N., Soliman B., and Hassan M., (2007): Effect of Azotobacter and different source of organic matter on growth and nutrition of Valencia seedling in new soil. J. Agric. Sci. Mans. Univ., 32(10): 8553 - 8573.
Ambesh B., Roy A., Ngomle S., Bhattacharya P., and Meena V., (2017): Isolation and evaluation of Azotobacter spp. from different crop rhizosphere. Int. J. Curr. Microbiol. App. Sci., 6(4): 883-888.
Amutha R., Karunakaran S., Dhanasekaran S., and Monika K., (2014): Isolation and mass production of biofertilizer (Azotobacter and phosphobacter). Int. J. of Lat. Res. in Sci. and Tec., 3(1): 79-85.
Andjelkovic S., Vasic T., Radovica J., and Snezana B., (2018): Abundance of Azotobacter in the soil of natural and artificial grasslands. Solut. Proj. Sustain. Soil Manage. 172.
Becking J., (2006): The family Azotobacter-aceae, in: The Prokaryotes: Volume 6: Proteobacteria: Gamma Subclass, eds Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. (New York, NY: Springer), 759–783.
Bloom P., and Skyllberg U., (2012): Soil pH and pH buffering. In Huang, Pan Ming; Li, Yuncong; Sumner, Malcolm E. (eds.). Handbook of soil sciences: properties and processes (2nd ed.). Boca Raton, FL: CRC Press. pp. 19–1 to 19–14.
Colco R., (2005): Gram Staining. Current Protocols in Microbiology. Appendix 3 (1): Appendix D3C. doi: 10.1002/9780471729259.mca03cs00.
Dar G., Dar S., Bhat R., and Dervash M., (2020): Azotobacter as biofertilizer for sustainable soil and plant health under saline environmental conditions. In: Microbiota and Biofertilizers, A sustainable sontinuum for plant and soil health (pp. 231-254), Publisher: Springer Cham.
Elsyaed B., and Nady M., (2013): Bioremed-iation of pendimethalin contaminated soil. J. Microbiol. Res., 7(21): 2574-2588.
Eric L., and Thomas P., (2009): The hydrodyn-amics of swimming microorganisms. Rep., on Prog. Phys., 72 (9): 096601.
Fromme A., Funke F., Merz J., and Schembecker G., (2011): Conductance densities and fluidities of solutions of silver nitrate and of ammonium nitrate at 35 °C. J Chem, 31 (7): 617-630.
Geisler E., Bogler A., Bar-Zeev E., and Rahav E., (2020): Heterotrophic nitrogen fixation at the hyper-eutrophic Qishon river and estuary system. Fron., Microbiol. (11), Article 1370.
Imtiaz-Rashid R., Mujawar L., Shahzad T., Almeelbi T., Ismail I., and Oves M., (2016): Bacteria and fungi can contribute to nutrients bioavailability and aggregate formation in degraded soils. Microbiol. Res., (183): 26-41.
Iqbal A., Amanullah H., Mazhar I., (2018): Integrated use of phosphorus and organic matter improve fodder yield of Moth bean (Vigna aconitifolia (Jacq.) under irrigated and dryland conditions of Pakistan. J. AgriSear, 4(1): 10-15.
Islam Z., Sharif D., and Hossain M., (2008): A comparative study of Azotobacter spp. from different soil samples. J. Soil. Nat., 2(3):16-19.
Jones A., Breuning-Madsen H., and Brossard M., (2013): Soil atlas of Africa, European commission, publications office of the European Union, Brussels, Belgium.
Kizilkaya R., (2009): Nitrogen fixation capacity of Azotobacter spp. strains isolated from soils in different ecosystems and relationship between them and the microbiological properties of soils. Journal of Environmental Biology, 30(1):73-82.
Kumar M., Bauddh K., Manish S., Sanger P., and Singh R., (2015): Increase in growth, productivity and nutritional status of wheat (Triticum aestivum) and enrichment in soil microbial population applied with biofertilizers entrapped with organic matrix. J. Plant Nutr., 260-276.
Kumar R., Bhatia R., Kukreja K., Behl R., Dudeja S., and Narula N. (2007). Establishment of Azotobacter on plant roots: chemotactic response, development and analysis of root exudates of cotton (Gossypium hirsutum L.) and wheat (Triticum aestivum L.). J bas. Microbiol., 47(5), 436-439.
Lima M., Sasaki M., Marinho O., and Rocha F., (2020): Spot test for fast determination of hydrogen peroxide as a milk adulterant by smartphone-based digital image colorimetry. Microchem. J., 157: 105042.
MacFaddin J., (2000). Biochemical tests for identification of medical bacteria, 3rd ed. Lippincott Williams & Wilkins, Philadelphia, PA.
Mikolajewicz N, and Komarova S., (2019): Simultaneous fluorescent recordings of extracellular ATP and intracellular calcium in mammalian cells. Bio Protoc, 20; 9(10): e3242. doi: 10.21769/BioProtoc.3242.
Mwendwa S., (2022): Revisiting soil texture analysis: Practices towards a more accurate Bouyoucos method. Heliyon, Heliyon, 8(7): e09395.
Neina D., (2019): The role of soil pH in plant nutrition and soil remediation. App. and Environ. Soil Sci. V. 2019, Article 5794869.
Noar J., Loveless T., Navarro-Herrero J., Olson J., and Bruno-Bárcena J., (2015): Aerobic hydrogen production via nitrogenase in Azotobacter vinelandii CA6. J. App. Environ Microbiol, 81(13); 4507–4516.
O’Brien F., Almaraz M., Foster M., Hill A., Huber D., King E., and Leopold M., (2019): Soil salinity and pH drive soil bacterial community composition and diversity along a lateritic slope in the Avon river critical zone observatory, Western Australia. Fron. Microb.,
Othaman C., Isa M., Ismail R., Ahmad M., and Hui C., (2020): Factors that affect soil electrical conductivity (EC) based system for smart farming application. AIP Conf. Proc. 2203, 020055 https://doi.org/10.1063/1.5142147.
Rice C., Pires C., Lin J., and Sarto M., (2021): Chapter three: soil organic carbon assessment methods. Soil Health Series: V. 2 Laboratory Methods for Soil Health Analysis, (38-51), Soil Sci., Soc., of Ame., Inc.
Rodriguesa A., Ladeirab L., and Arrobasa M., (2018): Azotobacter-enriched organic manures to increase nitrogen fixation and crop productivity. Eur. J. Agr., 93: 88-94.
Rysan L., and Sarec O., (2008): Research of correlation between electric soil conductivity and yield based on the use of GPS technology. Res.in Agr. Eng., 54(3): 136–147.
Sambrook J., Fritsch F., and Maniatis T., (2001): Molecular cloning; A Laboratory Manual, Cold Spring Harbor Laboratory Press. New York.
Sumbul A., Ansari R., Rizvi R., and Mahmood I., (2020): Azotobacter: A potential bio-fertilizer for soil and plant health management. Saudi J. Biol. Sci., 27(12): 3634 – 3640.
Thomas G., (2018): Soil pH and soil acidity. Methods of Soil Analysis. SSSA Book Series. 475–490.
Ujah I., Achikanu C., and Nsude C., (2021): Isolation and characterization of Azotobactr species from three soil samples in Enugu state Nigeria, IOSR J. Biotech. and Biochem., 7(5): 14-18.
Umer M., Younis F., Abdo H., Karim N., and Abdulrahman N., (2021): Spatial distribution of heavy metals for environmental and agricultural assessment in Badinan province, Kurdistan Region, Iraq. Mat. Tod., 2 (5): 1872-1878.
USDA, (2014): Soil electrical conductivity, Soil Health: Guide for educators. United State, Department of Agriculture, Natural Resources Conservation Services.
Vashist H., Sharma D., and Gupta A., (2013): A review on commonly used biochemical test for bacteria. Int. J life Sci., 1(1): 20-30.
Vitolo M., (2022): Notes on urea hydrolysis by urease. J. Phar. and Pharmaceu. Sci., 11(3): 96-135.
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