THE LACK OF CORRELATION IN TYPE 2 DIABETES MELLITUS BETWEEN NON-CPG METHYLATION AND DIABETES RISK FACTORS, LIPID PROFILE, KIDNEY FUNCTION TEST, HBA1C, AND RANDOM BLOOD SUGAR
DOI:
https://doi.org/10.25271/sjuoz.2024.12.2.1259Keywords:
Non-CPG methylation, type 2 diabetes, ABCC8, CAPN10 and hypermethylationAbstract
Background: Chronic hyperglycemia and insulin resistance are two hallmarks of type 2 diabetes mellitus (T2DM), a complicated metabolic disease. The purpose of the current investigation was to identify Non-CPG methylation in the ABCC8 and CAPN10 promoter regions.
Method: Fifty people were divided into two groups: thirty-five were diagnosed with type 2 diabetes mellitus, and fifteen were control group. Utilizing direct bisulfite sequencing to identify Non-CPG methylation in the promoter region and determine the extent of DNA methylation. To ascertain whether a result was statistically significant at the level of significance 0.05, the T-independent test, Spearman's correlation, and Chi square tests were performed.
Results: Hypermethylation of DNA has been found in the diabetes promoter region of the CAPN10 and ABCC8 genes compared to the healthy group. Furthermore, Non-CPG methylation in both genes and the statistically significant CAPN10 gene has not been linked to risk factors or biochemical indicators.
Conclusion: This study concluded that the biochemical biomarkers and risk factors did not influence Non-CPG methylation of selected genes in type 2 diabetes mellitus
References
Ahmed, S. A. H., Ansari, S. A., Mensah-Brown, E. P., & Emerald, B. S. (2020). The role of DNA methylation in the pathogenesis of type 2 diabetes mellitus. Clinical epigenetics, 12, 1-23.
Bansal, A., & Pinney, S. E. (2017). DNA methylation and its role in the pathogenesis of diabetes. Pediatric diabetes, 18(3), 167-177.
Barrès, R., Osler, M. E., Yan, J., Rune, A., Fritz, T., Caidahl, K., ... & Zierath, J. R. (2009). Non-CPG methylation of the PGC-1α promoter through DNMT3B controls mitochondrial density. Cell metabolism, 10(3), 189-198.
Davegårdh, C., García-Calzón, S., Bacos, K., & Ling, C. (2018). DNA methylation in the pathogenesis of type 2 diabetes in humans. Molecular metabolism, 14, 12-25.
Dayeh, T., Volkov, P., Salö, S., Hall, E., Nilsson, E., Olsson, A. H., ... & Ling, C. (2014). Genome-wide DNA methylation analysis of human pancreatic islets from type 2 diabetic and non-diabetic donors identifies candidate genes that influence insulin secretion. PLoS genetics, 10(3), e1004160.
Harrison, A., & Parle-McDermott, A. (2011). DNA methylation: a timeline of methods and applications. Frontiers in genetics, 2, 74.
Kim, M. (2019). DNA methylation: a cause and consequence of type 2 diabetes. Genomics & informatics, 17(4).
Kovacova, V., & Janousek, B. (2012). Bisprimer—a program for the design of primers for bisulfite-based genomic sequencing of both plant and Mammalian DNA samples. Journal of Heredity, 103(2), 308-312.
Kresse, S. H., Brandt-Winge, S., Pharo, H., Flatin, B. T., Jeanmougin, M., Vedeld, H. M., & Lind, G. E. (2023). Evaluation of commercial kits for isolation and bisulfite conversion of circulating cell-free tumor DNA from blood. Clinical Epigenetics, 15(1), 151.
Li, Y., & Tollefsbol, T. O. (2011). DNA methylation detection: bisulfite genomic sequencing analysis. Epigenetics protocols, 11-21.
Low, H. C., Chilian, W. M., Ratnam, W., Karupaiah, T., Md Noh, M. F., Mansor, F., ... & Pung, Y. F. (2023). Changes in mitochondrial epigenome in type 2 diabetes mellitus. British Journal of Biomedical Science, 80, 10884.
Mutize, T., Mkandla, Z., & Nkambule, B. B. (2018). Global and gene-specific DNA methylation in adult type 2 diabetic individuals: a protocol for a systematic review. Systematic Reviews, 7(1), 1-5.
Parrillo, L., Spinelli, R., Nicolò, A., Longo, M., Mirra, P., Raciti, G. A., ... & Beguinot, F. (2019). Nutritional factors, DNA methylation, and risk of type 2 diabetes and obesity: perspectives and challenges. International journal of molecular sciences, 20(12), 2983.
Patch, A. M., Flanagan, S. E., Boustred, C., Hattersley, A. T., & Ellard, S. (2007). Mutations in the ABCC8 gene encoding the SUR1 subunit of the KATP channel cause transient neonatal diabetes, permanent neonatal diabetes or permanent diabetes diagnosed outside the neonatal period. Diabetes, Obesity and Metabolism, 9, 28-39.
Prasad, R. B., & Groop, L. (2015). Genetics of type 2 diabetes—pitfalls and possibilities. Genes, 6(1), 87-123.
Raciti, G. A., Desiderio, A., Longo, M., Leone, A., Zatterale, F., Prevenzano, I., ... & Beguinot, F. (2021). DNA methylation and type 2 diabetes: novel biomarkers for risk assessment?. International Journal of Molecular Sciences, 22(21), 11652.
Smail, H. O., & Mohamad, D. A. (2022). Identification DNA Methylation Change of ABCC8 Gene in Type 2 Diabetes Mellitus as Predictive Biomarkers. ARO-THE SCIENTIFIC journal of koya university, 10(1), 63-67.
Smail, H. O., & Mohamad, D. A. (2023). Identification of DNA methylation of gene changes in the patients with type 2 diabetes mellitus as a predictive biomarker instead of HbA1c, random blood sugar, lipid profile, kidney function test, and some risk factors. Endocrine Regulations, 57(1), 221-234.
Walaszczyk, E., Luijten, M., Spijkerman, A. M., Bonder, M. J., Lutgers, H. L., Snieder, H., ... & van Vliet-Ostaptchouk, J. V. (2018). DNA methylation markers associated with type 2 diabetes, fasting glucose and HbA1c levels: a systematic review and replication in a case–control sample of the Lifelines study. Diabetologia, 61(2), 354-368.
Willmer, T., Johnson, R., Louw, J., & Pheiffer, C. (2018). Blood-based DNA methylation biomarkers for type 2 diabetes: potential for clinical applications. Frontiers in endocrinology, 9, 744.
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Harem O. Smail
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License [CC BY-NC-SA 4.0] that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work, with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online.
