The Effect of the Different Concentration and Proportion of Mixtures of Actara Insecticide and Ginger Oil in the Mortality Rate of Larvae of Greater Wax Moth, Galleria Mellonella L.

Abstract

During this study the toxic effect of Actara and ginger oil of Zingiber officinale, individually or in combination, on the 3^rd and 5^th instar larvae of greater wax moth, Galleria mellonella L. was investigated. The percentages of mortality of 3^rd and 5^th instars larval stage exposed for Actara, at concentrations of 0.1, 0.15 and 0.2 µl/ larva, after 24 hours exposure were 17.50, 25.00 and 32.50 %, and 10.00, 12.50 and 17.50 respectively. The percentages of mortality of 3^rd and 5^th  instars larval stage exposed to mixture 1:1 of Actara insecticide and ginger oil at concentrations of  0.1, 0.15 and 0.2 µl/ larva were 26.60, 40.00 and 50.00% (for 3^rd instar), and 20, 26.60 and 33.30% (for 5^th instar) respectively, after exposure period of 24 hours. While the mortality of 3^rd and 5^th instars larval stage exposed to the mixing ratio 1:2 (Actara insecticide and ginger oil) of the same concentrations and same exposure period mentioned previously, were 36.60, 46.00 and 63.30 % (for 3^rd instar), and 26.60, 36.60 and 46.60% (for 5^th instar), respectively. Whereas in the mixing ratio 1:3(Actara insecticide and ginger oil) of same concentrations and same exposure period mentioned previously, the mortality (3^rd and 5^th instars larvae) were 53.30, 63.00 and 90.00 % (for 3^rd instar), and 33.30, 46.60 and 63.00% (for 5^th instar), respectively. The synergistic ratios for 3^rd and 5^th instars larval stage exposed to mixtures 1:1, 1:2 and 1:3 (different concentrations of Actara insecticide and ginger oil), were 1.25, 1.6 and 2.4 (for 3^rd instar), and 1.88, 3.50 and 4.01 (for 5^th instar), respectively, after 24 hours of exposure.  This indicates that a combination of different concentrations of the Actara insecticide with ginger oil produced synergistic effect.