Correlation Between Plasma Endothelin-1, Blood Glucose and Serum Calcium in Male Rats Instillated with Bleomycin
Keywords:
: Endothelin-1, Bleomycin, blood glucos, calcium ionsAbstract
Endothelin-1 is profibrotic by stimulating fibroblast replication, migration, contraction, collagen synthesis and secretion while decreasing collagen degradation.In the present study,20male rats weighing 240 grams and 10-12 weeks old were utilized.Pulmonary fibrosis was induced by a single instillation of bleomycin (90 mg/kg).After 7 days of instillation, elevated plasma ET-1 was considered as bleomycin induced pulmonary fibrosis. High dose of bleomycin instillation caused significant increase in plasma ET-1as compared with normal saline instillation. Blood glucose and serum calcium were also markedly elevated. Statistical analysis using Pearson coefficient of correlation revealed that there was a positive relationship between plasma ET-1 and blood sugar (r=0.7). The same correlation was detected between plasma ET-1 and serum calcium (r=0.7).The present study concluded thatthere is a strong correlation between plasma ET-1 and blood glucose and it may be due to decreaseof insulinsensitivity, and inhibits β-cells to release insulin .In addition,the role of calcium ions cannot be excluded in raising blood glucose by ET-1.
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Fagan, K.A; MeMurtry, I.F and Rodman,D.M., (2001) .Role of endothelin-1 in lung disease. Respir. Res, 2: 90-101.
Ferri, C; Pittoni, V ; Piccoli, A; Laurenti, O; Cassone, M.R; Bellini, C; Properzi, G; Valesini, G; Mattia, G.D and Santucci, A., (1995) .Insulin stimulates endothelin-1 secretion from human endothelial cells and modulates its circulating levels in vivo. Journal of Clinical Endocrinology and Metabolism.Vol.80,No.3.
Hamaguchi, Y; Nishizawa, Y; Yasui, M; Masegawa, M; Kaburagi, Y; Komura; Nagaoka, T; Saito, E; Shimada, Y; Takehara, K; Kadono, T; Steeber, D.A; Tedder, T.F and Sato, S.,(2002). Intracellular adhesion molecule-1 and L-slsctin regulate bleomycin-induced lung fibrosis. America. Journal of Pathology.161: 1607-1618
Keane, M. P; Belperio, G.A; Arenberg D. A; Burdick, M. D; Xu, Z. J; Xue, Y. Y; and Strieter, R. M., (1999).IFN-γ inducible protein -10 attenuates bleomycin- induced pulmonary fibrosis via Inhibition of angiogenesis. The journal of immunology, 163: 5686 – 5692.
Mutsaers, S.E; Foster, M.L; Chambers, R.C; Laurent, G.J and McAnulty, R.J., (1998).Increased endothelin-1 and its localization during the development of bleomycin induced pulmonary fibrosis in rats.Am.J.Respire.Cell Med.Biol.18:611-619.
Park.S.H; Saleh, D; Giaid, A and Michel, R.P., (1997).Increased endothelin-1 in bleomycin-induced pulmonary fibrosis and the effect of an endothelin receptor anragonist.Am.J.Respir.Crit.Care Med.156:600-608.
Rossi, G. P; Seccia, T. M; Albertin, G; and Pessina, A. C., (2000) .Measurement of endothelin: Clinical and research use.Ann. ClinBiochem, 37: 608- 626.
SerranoMollar,A;Closa,D;Cortijo,J;Marcillo,E.J;Prats,N;Gironella,M;Panes,J;Rosell,Jand Bulbena,O (2002).P-selectin up regulation in bleomycin –induced lung injury in rats :effect of N-acetyl-L-cysteine Thorax 57:629-634
ShiWen,X;Chen,Y;Denton,C.P;Renzoni,M.E,Bou_Gharios,G,Pearson,J.D,DuBios,M.D.M;Black,C.M;Leask,A and Abraham, D.J., (2004).Endothelin-1 promotes myofibroblast induction through receptor via a rac/phosphoinositide 3-kinase /Akt-pathway and is essential for the enhanced contractive fibrotic fibroblasts.Mol.Biol.Cell.15:2707-2719.
Sun, G; Stacey, M.A, Bellini.A; Marini, M.andMattoli, S., (1997).Endothelin-1 induced bronchial myofibroblast differentiation Peptides.18:1449-1451.
Syiem, D; Syngai, G; Khup, P.Z; Khongwir, B.S; Kharbnli, B. and Kayang, H., (2002).Hypoglycemic effects of Potentilliafulgens L. in normal and alloxan-induced diabetic mice.Journal of Ethnopharmacology, 83: 51-56
Teder, P and Noble, P, W., (2000) .A cytocine reborn? Endothelin- 1 in pulmonary inflammation and fibrosis Am. J Resp. Cell mol. Biol vol. 23, 7- 10.
Teuscher, A.U; Lerch, M; Shaw, S; Pacini, G; Ferrari, P. and Weidmann, P., (1998). Endothelin-1 infusion inhibits insulin responsiveness in normal men. J. Hypertension, 16:1279-1284
Villaschi, S and Nicosia, R.F., (1994) .paracrine interaction between fibroblast and endothelial cells in a serum free culture model: modulation of angiogenesis and collagen gel contraction Lab Invest. 71: 291- 299 .
Wang,H-D;Yamaya,M;Okinaga,S;Jia,Y-X,Kamanaka,M;Takahashi,H;Guo,L-Y,Ohrui,T and Saski,H.A(2002).Bilirubinamelioratesbleomycin –induced pulmonary fibrosis in rats. Am.J.Res.Crit.Care Med.165:406-411.
Wilkes, J, J; Hevenda, A; Olefsky, J (2003). Chronic endothelin- 1 treatment leads to insulin resistance in vivo. Diabetes, vol. 32, 1904- 1909.
Yanagisawa, M; Kurihara, H; Kimura, S; ; Tombbe, Y; Kobayashi, M; Mitsui Y; Yazaki, Y; Goto, K; and Masaki, T., (1988) .A novel potent vasoconstrictor peptide produced by vascular endothelial cells Nature; 332: 411- 415
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2013-06-30
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MAULOOD, I. M., JAF, H. N. H., & MAHMUD, A. M. R. (2013). Correlation Between Plasma Endothelin-1, Blood Glucose and Serum Calcium in Male Rats Instillated with Bleomycin. Science Journal of University of Zakho, 1(1), 164–169. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/60
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