Protective Role of Melatonin in L-Name Induced Hypertension in Male Albino Rats

  • Aveen R. Khdhr Soran University
  • Almas M. R. Mahmud University of Salahaddin
Keywords: melatonin, L-NAME, hypertension, NO, oxidative stress


The objective for the present study is to investigate the effects of melatonin (MEL) on systolic blood pressure (SBP), some biochemical parameters; serum (malondialdehyde (MDA), superoxide dismutase (SOD), reduced glutathione (GSH), nitric oxide (NO)) in NG-nitro-L-arginine methyl ester hydrochloride (L-NAME) treated rats. The male albino rats divided into five groups treated for 4 weeks: Group 1: Control rats. Group 2: L-NAME (35 mg/100 ml drinking water). Group 3: L-NAME (35 mg/100 ml drinking water) + melatonin (30 mg/Kg diet). Group 4: L-NAME (35 mg/100 ml drinking water) + melatonin (60 mg/Kg diet). Group 5: L-NAME (35 mg/100 ml drinking water) + melatonin (120 mg/Kg diet). A significant elevation in SBP and serum MDA were detected in L-NAME treated rats. Co-administration of melatonin with L-NAME prevented increasing in SBP and serum level of MDA in a dose dependent manner. On the other hand serum levels   of SOD and GSH were decreased in response to L-NAME treatment, while, co-treatment with melatonin increased SOD and GSH in a dose dependent manner. The decrease serum NO level in response to L-NAME was significantly increased by melatonin but its level was decreased by increasing melatonin doses. In conclusion:  L-NAME induced hypertension model was associated with decreased NO level, interestingly; melatonin increased serum NO in L-NAME treatments, but with increasing dose of MEL, NO level was decreased. Furthermore; MEL through its antioxidant properties reduced oxidative stress and prevented lipid peroxidation.

Author Biographies

Aveen R. Khdhr, Soran University

Dept. of Biology, Faculty of Science, Soran University, Kurdistan Region - Iraq

Almas M. R. Mahmud, University of Salahaddin

Dept. of Biology, College of Science, University of Salahaddin, Kurdistan Region – Iraq.


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How to Cite
Khdhr, A., & Mahmud, A. (2016). Protective Role of Melatonin in L-Name Induced Hypertension in Male Albino Rats. Science Journal of University of Zakho, 4(1), 18 - 24. Retrieved from
Science Journal of University of Zakho