Protective Role of Melatonin in L-Name Induced Hypertension in Male Albino Rats
Keywords:
melatonin, L-NAME, hypertension, NO, oxidative stressAbstract
The objective for the present study is to investigate the effects of melatonin (MEL) on systolic blood pressure (SBP), some biochemical parameters; serum (malondialdehyde (MDA), superoxide dismutase (SOD), reduced glutathione (GSH), nitric oxide (NO)) in NG-nitro-L-arginine methyl ester hydrochloride (L-NAME) treated rats. The male albino rats divided into five groups treated for 4 weeks: Group 1: Control rats. Group 2: L-NAME (35 mg/100 ml drinking water). Group 3: L-NAME (35 mg/100 ml drinking water) + melatonin (30 mg/Kg diet). Group 4: L-NAME (35 mg/100 ml drinking water) + melatonin (60 mg/Kg diet). Group 5: L-NAME (35 mg/100 ml drinking water) + melatonin (120 mg/Kg diet). A significant elevation in SBP and serum MDA were detected in L-NAME treated rats. Co-administration of melatonin with L-NAME prevented increasing in SBP and serum level of MDA in a dose dependent manner. On the other hand serum levels of SOD and GSH were decreased in response to L-NAME treatment, while, co-treatment with melatonin increased SOD and GSH in a dose dependent manner. The decrease serum NO level in response to L-NAME was significantly increased by melatonin but its level was decreased by increasing melatonin doses. In conclusion: L-NAME induced hypertension model was associated with decreased NO level, interestingly; melatonin increased serum NO in L-NAME treatments, but with increasing dose of MEL, NO level was decreased. Furthermore; MEL through its antioxidant properties reduced oxidative stress and prevented lipid peroxidation.
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Badyal, D.K., Lata H. and Dadhich A.P., (2003). Animal models of hypertension and effects of drugs. Indian Journal of pharmacology. 35: 349-362.
Chuang, J.I., Chen S.S. and Lin M.T., (1993). Melatonin decreases brain serotonin release, arterial pressure and heart rate in rats. Pharmacology. 47: 91-97.
Deniz, E., Colakoglu N., Sari A., Sonmez M.F., Tugrul I., Oktar S., Ilhan S. and Sahna E., (2006). Melatonin attenuates renal ischemia-reperfusion injury in nitric oxide synthase inhibited rats. Acta Histochem. 108(4): 303-9.
Ding, Y., Gonick H.C. and Vaziri N.D., (2001). Lead- induced hypertension: increased hydroxyl radical production. Am J Hypertension. 14: 169-175.
Girouard, H., Chulak C.H., Lejossec M., Lamontagne D. and De Champlain J., (2001). Vasorelaxant effects of the chronic treatment with melatonin on mesenteric artery and aorta of spontaneously hypertensive rats. J Hypertens. 19: 1369-1377.
Guyton, A.C. and Hall J.E., (2006). Textbook of medical physiology.12th edition. W.B. Saunders Company, Philadelphia.
Hara, M., Yoshida M., Nishijima H., Yokosuka M., Iigo M., Ohtani-kaneko R., Shimada A., Hasegawa T., Akama Y. and Hirata K., (2001). Melatonin, a pineal secretory product with antioxidant properties, protects against cisplatin-induced nephrotoxicity in rats. J pineal Res. 30: 129-138.
Huk, I., Nanobashivili J., Neumayar C., Punz A., Mueller M., Afkhampour K., Mittlboek M., Losrt U., Polterauer P., Rath E., Patton S. and Malinski T., (1997). L-arginine treatment alters the kinetics of nitric oxide and superoxide release and reduces ischemia / reperfusion injury in skeletal muscle. Circulation. 34(7):63-69.
K-Laflamme, A., Wu L., Foucart S. and de Champlain J., (1998). Impaired basal sympathetic tone and alpha 1-adrenergic responsiveness in association with the hypotensive effect of melatonin in spontaneously hypertensive rats. Am J Hypertens. 11: 219-229.
Kojsova, S., Jendekova L., Zicha J., Kunes J., Andriantsitohaina R. and Pechanova O., (2006). The effect of different antioxidants on nitric oxide production in hypertensive rats. Physiol Res.55 (1): S3-S16.
Kopkan, L. and Majid S.A., (2005). Superoxide contributes to development of salt sensitivity and hypertension induced by nitric oxide deficiency. Hypertension. 46: 1026.
Kunes, J., Hojna S., Kadlecova M., Dobesova Z., Rauchova H., Vokurkova M., Loukotova J., Pechanova O. and Zicha J., (2004). Altered balance of vasoactive systems in experimental hypertension; the role of relative NO deficiency. Physiological Research. 53: S 23-S 34.
Kurtz, A. and Wagner C., (1998). Role of nitiric oxide in the control of renin secretion. Am J Physiol Renal Physiol. 275: F849- F862.
Landmesser, U., Dikalov S., Price S.R., McCann L., Fukai T., Holland S.M., Mitch W.E. and Harrison D.G., (2003). Oxidation of tetrahydrobiopterin leads to uncoupling of endothelial cell nitric oxide synthase in hypertension. J Clin Invest. 111: 1201–1209.
Pacher, P., Beckman J.S. and Liaudet L., (2007). Nitric oxide and peroxynitrite in health and disease. Physiol. Rev. 87: 315-424.
Paulis, L. and Simko F., (2007). Blood pressure modulation and cardiovascular protection by melatonin: Potential mechanism behind. Physiol. Res. 56: 671-687.
Pechanova, O., Dobesova Z., Cejka J., Kunes J. and Zicha J., (2004). Vasoactive systems in L-NAME hypertension: the role of inducible NO synthase. J Hypertens. 22: 167-173.
Pechanova, O., Zicha J., Paulis L., Zenebe W., Dobesova Z., Kojsova S., Jendekova L., Sladkova M., Dovinova I., Simko F. and Kunes J., (2006). Effect of N-acetylcysteine and melatonin in adult SHR with established hypertension. Submitted to Eur J Pharmacol. Pediatr Nephrol. 22: 2011–2022.
Pierpaoli, W. and Regelson W., (1994). Pineal control of aging: effect of melatonin and pineal grafting on aging mice. Proc Nat / Acad Sci U S A. 91: 787-791.
Rodriguez, C., Mayo J.C., Sainz R.M., Antoli I., Herrera F., Martin V. and Reiter R.J., (2004). Regulation of antioxidant enzymes: a significant role for melatonin. J. Pineal. Res. 36: 91-9.
Tan, D.X., Reiter R.J., Manchester L.C., Yan M.T., El-Sawi M., Sainz R.M., Mayo J.C., Kohen R., Allegra M. and Hardeland R., (2002). Chemical and physical properties and potential mechanisms: Melatonin as a broad spectrum antioxidant and free radical scavenger. Curr Top Med Chem. 2: 181–197.
Uzun, H., Simsek G., Aydin S., Unal E., Karter Y., Yelmen N.K., Vehid S., Curgunlu A. and Kaya S., (2005). Potential effects of L-NAME on alcohol-induced oxidative stress. World J Gastroenterol.11(4):600-604.
Wu, W.R. and De Champlain J., (1998). Enhanced inhibition by melatonin of a-adrenoceptorinduced aortic contraction and inositol phosphate production in vascular smooth muscle cells from spontaneously hypertensive rats. J Hypertens. 16: 339-347.
Zanchi, A., Schaad N.C., Osterheld M.C., Grouzmann E., Nussberger J., Brunner H.R. and Waeber B., (1995). Effects of chronic NO synthase inhibition in rats on renin-angiotensin system and sympathetic nervous system. American Journal of Physiology. 268: H2267- H2273.
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