Assessment of Genetic Diversity and Population Structure of Some Soft and Hard Wheat Varieties Based on SSR Marker
DOI:
https://doi.org/10.25271/sjuoz.2020.8.3.732Keywords:
Triticum sp., genetic diversity, hard and soft wheat, population, SSRs markerAbstract
Wheat (Triticum spp.) is one of the most important cereal crops in Iraq and the world. It includes many species and varieties. The two major cultivated species of wheat are, durum wheat (Tritium durum Desf.) which is tetraploid (2n= 28) and the common wheat (Triticum aestivum L.) which is hexaploid (2n = 42). Ten wheat varieties from both species were examined using ten Simple sequence repeat (SSR) markers (WMC17, WMC20, WMC21, WMC24, WMC25, WMC48, WMC50, WMC283, Xgwm11 and Xgwm626). Various genetic parameters were calculated using Power Marker V3.25 software. A total of 156 alleles were detected in both species. The gene diversity in wheat varieties from both species collectively varied from 0.85 to 1.00, which indicates considerable genetic diversity in the examined varieties. All markers used in this study were highly informative and the polymorphic information content (PIC) values were higher than 0.50 in all loci. Hence all markers are considered useful for genetic diversity studies in wheat’s populations. The dendrogram separated the populations into two main clades and many subgroups. Azadi variety was simplicifolious. This study confirms the discriminating power of SSR typing and its usefulness for comparison within hard and soft wheat populations.
References
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Suresh S, Chung JW, Cho GT, Sung JS, Park JH, Gwag JG,
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Ya Narantsetseg, Sebastin Raveendar, Bayarsukh N, Myagmarsuren, Ya, Jung-Ro Lee, Kyung-Jun Lee, Myoung-Jae Shin, Gyu-Taek Cho, Kyung-Ho Ma, Gi-An Lee (2017) Genetic Diversity and Population Structure of Mongolian Wheat based on SSR Markers: Implications for Conservation and Management. Plant Breed. Biotech. 5(3):213~220.
Yadav, M. K., & Chand, P. (2018). Assessment of Genetic Diversity among Twenty Indian Wheat (Triticum aestivum L.) Cultivars using Simple Sequence Repeat (SSR) Markers. Int. J. Curr. Microbiol. App. Sci, 7(3), 1708-1717.
Zarei Abbasabad E, Mohammadi SA, Moghaddam M, Jalal Kamali MR. (2016). Analysis of genetic diversity, population structure and linkage disequilibrium in Iranian wheat landraces using SSR markers. Plant Genetic Resources 1: 1-8.
Zhang D, Bai G, Zhu C, Yu J, Carver BF. (2010). Genetic diversity, population structure, and linkage disequilibrium in U.S. elite winter wheat. The Plant Genome 3: 117-127.
Allen, A. M., Winfield, M. O., Burridge, A.J., Downie, R. C., Benbow, H. R., Barker, G. L., Wilkinson, P.A., Coghill, J., Waterfall, C., Davassi, A. and Scopes, G. (2017). Characterization of a Wheat Breeders’ Array suitable for high‐ throughput SNP genotyping of global accessions of hexaploid bread wheat (Triticum aestivum L.). Plant biotechnology journal, 15(3): 390-401.
Babay E., Chaabane R., Mzid-Abdmouleh R., Ben Naceur M. (2015). Diversity of Tunisian bread wheat genotypes revealed by Morpho-agronomical and microsatellite markers. Bioscience Journal, 31(3), 701-708.
Bafghi R. Mollaheydari, A. Baghizadeh, Gh. Mohammadi-Nejad, B. Nakhoda(2014) Assessment of genetic diversity in Iranian wheat (Triticum aestivum L.) cultivars and lines using microsatellite markers. Journal of Plant Molecular Breeding (JPMB). Vol. 2. no.1, 74-89.
Baloch Faheem Shehzad, Ahmad Alsaleh, Muhammad Qasim Shahid, Vahdettin Citi, Luis E. Sâenz de Miera, Muhammad Aasim, Muhammad Azhar Nadeem, Husnu Aktaş, Hakan Özkan, Rüştü Hatipoğlu (2017). A Whole Genome DArTseq and SNP Analysis for Genetic Diversity Assessment in Durum Wheat from Central Fertile Crescent. PLoS ONE12(1): e0167821.
Barcaccia Gianni (2010). Molecular Markers for Characterizing and Conserving Crop Plant Germplasm Chapter 10 S.M. Jain and D.S. Brar (eds.), Molecular Techniques in Crop Improvement. Springer Science +Business Media B.V. 2009
Bassam, Brant J & Peter M Gresshoff (2007). Silver staining DNA in polyacrylamide gels nature protocols vol.2 No.11. 2649.
Desheva, G. & Kyosev, B. (2015). Genetic diversity assessment of common winter wheat (Triticum aestivum L.) genotypes. Emirates Journal of Food and Agriculture, 27(3), 283-290.
El-Fiki, A. & Adly, M. (2019). Molecular characterization
and genetic diversity in some Egyptian wheat (Triticum aestivum L.) using microsatellite markers. Potravinarstvo Slovak Journal of Food Sciences, 13(1), 100-108.
Hao C., Wang L., Ge H., Dong Y. and Zhang X. (2011). Genetic diversity and linkage disequilibrium in Chinese bread wheat (Triticum aestivum L.) revealed by SSR markers. PLoS One 18;6(2) e17279.
Kara Karima, Najla Mezghani, Olfa Saddoud Debbabi, Maher Madini, Malika Rached-Kanouni1, M’barek Ben Naceur (2016). Assessment of genetic diversity of wheat (Triticum aestivum L.) using agro-morphological characters and microsatellite markers. International Journal of Biosciences vol. 9, No. 4, p. 92-101.
Kara, K., Kanouni, M. R., Debbabi, O. S., & Naceur, M. B. (2017). Genetic diversity of bread wheat genotypes (Triticum aestivum L.) revealed by agromorphological characteristics and microsatellite SSR markers. Int J Res Eng Technol, 6, 178-182.
Kara Karima, Rached-Kanouni Malika and Ben Naceur M’barek (2018). Investigation of Genetic Diversity among Bread Wheat Cultivars (Triticum aestivum L.) Using SSR Markers. 14th LISBON International Conference on Agricultural, Biological, Environmental and Medical Sciences (LABEMS-18) (Lisbon, Portugal) 17-19.
Kesawat, Mahipal Singh, and Basanta Das Kumar. (2009). Molecular Markers: It’s Application in Crop Improvement. Journal of Crop Science and Biotechnology 12 (4):169–81.
Khan MK, Pandey A, Choudhary S, Hakki EE, Akkaya MS, Thomas G. 2014. From RFLP to DArT: molecular tools for wheat (Triticum spp.) diversity analysis. Genetic Resources and Crop Evolution 61: 1001-1032.
Khan Mohd Kamran, Anamika Pandey, George Thomas, Mahinur S. Akkaya, Seyit Ali Kayis, Yusuf Ozsensoy, Mehmet Hamurcu, Sait Gezgin, Ali Topal and Erdogan E. Hakki (2015). Genetic diversity and population structure of wheat in India and Turkey. AoB PLANTS: plv083-plv083.
Kumar, P., Yadava, R. K., Kumar, S., & Kumar, P. (2016). Molecular diversity analysis in Wheat genotypes using SSR markers. Electronic Journal of Plant Breeding, 7(2), 464-468.
Nei, Masotoshi (1973). Genetic distance between populations. The American Naturalist, 106 (949), 283-292.
Nei, Masotoshi (1987). Molecular evolutionary genetics. Columbia university press. New York. 512 pp.
Raj, R. Sandeep, Yama S. Vyas, Viral Kumar M. Baranda, Madhvi N. Joshi, Shradha Nand Tyagi, and Snehal B. Bagatharia (2017). Ascertaining narrow genetic base in commercial accessions of wheat commonly grown in Gujarat via molecular markers. Electronic Journal of Plant Breeding 8 (2): 558.
Röder, M.S.; Korzun, V., Gill, B.S., Wendehake, K., Pleaschke, J., Tixier, M., Leroy, P., Ganal, M.W. (1998). A microsatellite map of wheat. Genetics 149: 2007-2023.
Roussel V, Koenig J, Beckert M, Balfourier F. (2004). Molecular diversity in French bread wheat accessions
related to temporal trends and breeding programmes. Theor. Appl. Genet. 108: 920-930.
Salehi, Marzeih, Ahmad Arzani, Majid Talebi and Asad Rokhzadi (2018). Genetic diversity of wheat wild relatives using SSR markers. Genetika, Vol. 50, No1, 131-141.
Salem, K. F., Röder, M. S., & Börner, A. (2015). Assessing genetic diversity of Egyptian hexaploid wheat (Triticum aestivum L.) using microsatellite markers. Genetic resources and crop evolution, 62(3), 377-385.
Shewry, P. R. (2009). Wheat. Journal of experimental botany, 60 (6), 1537-1553.
Singh Piyusha and Naveen Kumar Singh (2018). SSR Molecular Marker are efficient tools for finding
Genetic Diversity in Bread Wheat. International Journal of Current Microbiology and Applied SciencesSpecial Issue-7 pp. 1098-11053
Slim Amine, Luciana Piarulli, Houda Chennaoui Kourda, Mustapha Rouaissi, Cyrine Robbana, Ramzi Chaabane, Domenico Pignone, Cinzia Montemurro and Giacomo Mangini (2019). Genetic Structure Analysis of a Collection of Tunisian Durum Wheat Germplasm. Int.J.Mol.Sci. 20, 3362.
Sokal. R. R. and C. D. Michener (1958). A statistical method for evaluating systematic relationships, Univ. Kans. Sci. Bull, 28, 1409–1438.
Sönmezoğlu Özlem Ates and Begüm Terzi1(2018). Characterization of some bread wheat genotypes using molecular markers for drought tolerance. Physiol Mol Biol Plants: 24(1):159–166.
Suresh S, Chung JW, Cho GT, Sung JS, Park JH, Gwag JG,
Baek HJ (2014). Analysis of molecular genetic diversity and population structure in Amaranthus germplasm using SSR markers. Plant Biosyst. 148: 635-644.
Ya Narantsetseg, Sebastin Raveendar, Bayarsukh N, Myagmarsuren, Ya, Jung-Ro Lee, Kyung-Jun Lee, Myoung-Jae Shin, Gyu-Taek Cho, Kyung-Ho Ma, Gi-An Lee (2017) Genetic Diversity and Population Structure of Mongolian Wheat based on SSR Markers: Implications for Conservation and Management. Plant Breed. Biotech. 5(3):213~220.
Yadav, M. K., & Chand, P. (2018). Assessment of Genetic Diversity among Twenty Indian Wheat (Triticum aestivum L.) Cultivars using Simple Sequence Repeat (SSR) Markers. Int. J. Curr. Microbiol. App. Sci, 7(3), 1708-1717.
Zarei Abbasabad E, Mohammadi SA, Moghaddam M, Jalal Kamali MR. (2016). Analysis of genetic diversity, population structure and linkage disequilibrium in Iranian wheat landraces using SSR markers. Plant Genetic Resources 1: 1-8.
Zhang D, Bai G, Zhu C, Yu J, Carver BF. (2010). Genetic diversity, population structure, and linkage disequilibrium in U.S. elite winter wheat. The Plant Genome 3: 117-127.
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Published
2020-09-30
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Fattah, Y. M., & Tayib, N. N. (2020). Assessment of Genetic Diversity and Population Structure of Some Soft and Hard Wheat Varieties Based on SSR Marker. Science Journal of University of Zakho, 8(3), 80–87. https://doi.org/10.25271/sjuoz.2020.8.3.732
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Science Journal of University of Zakho
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