Toxic Effects of Miller on Chromosomes and Sperms of Albino Males Mice
Keywords:
glyphosate, chromosome aberrations, sperm abnormalities, herbicideAbstract
The genotoxicity of Miller (glyphosate) herbicide was evaluated in males albino mice Mus muscles BALB/c strain. The tested parameters were chromosomal aberrations and sperm abnormalities to evaluate a possible damage effects on genetic material and sperms. The herbicide was administered orally to adult male laboratory mice in 4 different doses (0, 250, 500, and 750 mg/kg of body weight). The mice were killed at two periods: after 3 and 6 weeks. The results of chromosome aberration assay revealed that all the tested doses and periods induced structural chromosomal aberrations (CAs) such as centromeric gaps, chromatid gaps, delations, dicentric chromosomes and ring chromosomes. The results of sperm abnormality assay revealed that miller has the ability to induce sperm abnormalities in all doses used comparing to untreated mice, which represented by hookless sperm, swollen head sperm, amorphous head sperm, sperm without head, defective hook sperm, banana head sperm, double head sperm, sperm without tail, double tail sperm, bent midpiece defect and coiled tails.
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Wyrobek, A. J., J. A. Heddle, and W. R. Bruce (1975). Chromosomal abnormalities and the morphology of mouse sperm heads. Canadian Journal of Genetics and Cytology, 17(4): 675-681, 10.1139/g75-083
Wyrobek, A.J.(1979). Changes in mammalian sperm morphology after X-ray and chemical exposure. Genetics 59:105-119.
Yousef.M.I., M.H.Salem, H.Z.Ibrahim, S.Helmi, M.A.Seehyand K. Bertheussen(1995). Toxic effects of carbofuran and glyphosate on semen characteristics in rabbits. J. Environ. Sci. Health B 30:513-34
Zhao. S., A. Narang, J. Gierthy, and G. Eadon (2002). Detection and characterization of DNA adducts formed from metabolites of the fungicide ortho-phenylphenol. Journal of Agricultural and Food Chemistry, vol. 50, no. 11, pp. 3351–3358.
Anton.F.A., L.M.Cuadra., P.Gutierrez., E.Laborda and P. Laborda (1993). Degradation behavior of the pesticides glyphosate and diflubenzuron in water. Bull. Environ. Contam. Toxicol. 51:881-8.
Bhunya.S. and B.Behera (1988). Studies on the genotoxicity of monocrotophose (Astaf) im mammalian in vivo system. Mut. Res. 223: 287-293.
Buffin.D. and T.Jewell (2001). Health and environmental impacts of glyphosate: The implications of increased use of glyphosate in association with genetically modified crops. Pesticide Action Network UK. Edited by Pete Riley, Mary Taylor, Emily Diamand and Helen Barron
Cal/EPA (1992). Summary of toxicology data, glyphosate (isopropylamine salt). California Department of Pesticide Regulation, California Environmental Protection Agency, RP-11/3/92.
Clair, E., R.Mesnage, C.Travert and GÉ.Séralini (2012). A glyphosate-based herbicide induces necrosis and apoptosis in mature rat testicular cells in vitro and testosterone decrease at lower levels. Toxicol In Vitro. 26(2):269-79
Cornell University (2007). Pesticides in the environment. Pesticide fact sheets and tutorial, module 6. Pesticide Safety Education Program.
Duncan,D.B.( 1955). Multiple range and multiple F tests. Biometrics11:1–42.
El-Nahas.S.M., M.H.A. De-Hondt and H.E.Abdou (1989). Chromosome aberration in spermatogonia and sperm abnormalities in curacron-treated mice. Mut. Res. 222(4): 409-414.
Evans, E.P., G. Breckon and C.F. Ford. (1966). An air drying method for meiotic preparation from mammalian testis. Cytogenetic. 41(6): 317-321.
Holden.H.E., V.A.Ray, M.G.Wahrenburg, and J.D.Zelenski (1973). Mutagenecity studies with 6-mercapto purine. 1-Cytogenetic activity in vivo. Mutat. Res. 20: 257-263.
Hurtado de Catalfo Graciela, Astiz Mariana, Alaniz María J. T. de and Marra Carlos Alberto (2011).Frequently-used agrochemicals lead to functional and morphological spermatozoa alterations in rats. Journal of Toxicology and Environmental Health Sciences Vol. 3(7), pp. 180–192,
Jha.A.M., A.C. Singh and M.K. Bharti, (2002). Clastogenicity of carbazole in mouse bone marrow cells in vivo. Mut. Res. 521(1-2): 11-7.
Joshi.S.C., [ikkuR.Mathur, and N.Gulati (2007). Testicular toxicity of chlorpyrifos (an organophosphate pesticide) in albino rat, Toxicology and Industrial Health. 23: 439-444.
Kourakis.A., M.Mouratidou, G.Kokkinos, A.Barbouti, A. Kotsis, D. Mourelatos and J. Dozi-Vassiliades (1992). Frequencies of chromosomal aberrations in pesticides sprayers working in plastic green houses. Mut. Res. 279(2): 145-148.
Lander.F., L.E.Knudsen, M.O.Gamborg, H.Jarventaus, and H.Norppa (2000). Chromosome aberrations in pesticide-exposed greenhouse workers. Scandinavian Journal of Work, Environment and Health, vol. 26, no. 5, pp. 436–442.
Lioi. M.B, Maria R. Scarfi, Antonietta Santoro, Rocchina Barbieri , Olga Zeni, Francesca Salvemini, Dino Di Berardino, Matilde V. Ursini . (1998) .Cytogenetic damage and induction of pro-oxidant state in human lymphocytes exposed in vitro to glyphosate, vinclozolin, atrazine, and DPX-E9636. Environ Mol Mutagen 32(1):39-46.
Material safety data sheet (2010) Bayer glyphosate 450 herbicide. www.bayercropscience.com.au
Mathew.G., K.K.Vijayalaxmi and M.Abdul-Rahiman (1992). Methyl parathion induced sperm shape abnormalities in mouse. Mut. Res. 280(3): 169-173.
McMullan.P., J.Honeggar and E.Logusch (1990). Confined rotational crop study of glyphosate Part II. Quantitation, characterization and identification of glyphosate and its metabolites in rotational crops. Unpublished report no. MSL-981, 1990, submitted to U.S. Environmental Protection Agency by Monsanto Agricultural Labs. Reregistration Eligibility Decision (RED) Glyphosate; EPA.
Miller.A., J.A.Gervais, B.Luukinen, K.Buhl and D.Stone ( 2010). Glyphosate Technical Fact Sheet; National Pesticide Information Center, Oregon State University Extension Services.
Moxon, M. E. (2004).Glophosate acid: multigeneration reproduction toxicity in rats. Unpublished report noCTL/P/6332, study no. RR0784, 2000, submitted to WHO by Syngenta Crop Protection AG, Basel, Switzerland, prepared by Zeneca Agrochemicals, Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, England. Pesticide Residues in Food - Evaluations Part 2: Toxicological; International Programme on Chemical Safety, World Health Organization, Food and Agriculture Organization: Rome, Italy.
NTP (1992). NTP technical report on toxicity studies of glyphosate (case # 1071-83-6) administered in dosed feed to F344/N rats and B6C3F1 mice. NIH Publication 92-3135.
Peluso.M., A.Munnia, C.Bolognesi and S.Parodi (1998). P32-Postlabeling detection of DNA adducts in mice treated with the herbicide Roundup. Environmental and Molecular Mutagenesis, vol. 31, no. 1, pp. 55 59.
Poletta, G. L., A.Larriera, E.Kleinsorge and M.D.Mudry (2009).Genotoxicity of the herbicide formulation Roundup (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the Comet assay and the Micronucleus test. Mutation Research, vol. 672, no. 2, pp. 95–102,.
Prasad.S., S.Srivastava, M.Singh, and Y. Shukla, (2009). Clastogenic Effects of Glyphosate in Bone Marrow Cells of Swiss Albino Mice. Journal of Toxicology Volume 2009. Proteomics Laboratory, Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Lucknow 226001, India
Sharma, A.K. and Sharma, A. (1980). Chromosome techniques. (3rd ed.), Buterworths. London.
Sulaiman, K.M. (2000). The genetic and histological effects of cigarette smoke on albino laboratory mice Mus musculus. M.Sc. Thesis. College of education / University of Salahaddin-Erbil. Iraq
Swati.D.J.(2004). Occupational Female Reproductive Hazards. Master of Industrial Hygiene Programme, Institute of Science and Technology for Advanced Studies and Research, Science College. Journal of Health Management. 6 (2) 201-210.
Taha, H.M. (2000). The genetic effects of pesticides (Raxil and Granstar) on albino laboratory mice Mus musculus. M.Sc. Thesis. Dept. of Biol./College of education. University of Salahaddin-Erbil. Iraq.
U.S. EPA(1993), Reregistration Eligibility Decision, (RED) : Glyphosate EPA-738-R-93-014 U.S Environmental Protection Agency, Office of Prevention, Pesticides, and Toxic Substances, Office of Pesticide Programs, U.S. Government Printing Office: Washington, DC, 1993.
Van der Werf. H. M. G., ( 1996). Assessing the impact of pesticides on the environment, Agriculture, Ecosystems and Environment, vol. 60, no. 2-3, pp. 81–96,
Vencill, W. K. (2002). Herbicide Handbook, 8th ed. Weed Science Society of America: Lawrence, KS p 231-234.
WHO.(1996).Data Sheets on Pesticides: Glyphosate International Programme on Chemical Safety, World Health Organization, Food and Agriculture Organization: Geneva, Switzerland.
Wyrobek, A. J., J. A. Heddle, and W. R. Bruce (1975). Chromosomal abnormalities and the morphology of mouse sperm heads. Canadian Journal of Genetics and Cytology, 17(4): 675-681, 10.1139/g75-083
Wyrobek, A.J.(1979). Changes in mammalian sperm morphology after X-ray and chemical exposure. Genetics 59:105-119.
Yousef.M.I., M.H.Salem, H.Z.Ibrahim, S.Helmi, M.A.Seehyand K. Bertheussen(1995). Toxic effects of carbofuran and glyphosate on semen characteristics in rabbits. J. Environ. Sci. Health B 30:513-34
Zhao. S., A. Narang, J. Gierthy, and G. Eadon (2002). Detection and characterization of DNA adducts formed from metabolites of the fungicide ortho-phenylphenol. Journal of Agricultural and Food Chemistry, vol. 50, no. 11, pp. 3351–3358.
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2014-06-30
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Fattah, Y. M., & Mustafa, S. I. (2014). Toxic Effects of Miller on Chromosomes and Sperms of Albino Males Mice. Science Journal of University of Zakho, 2(1), 54–64. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/105
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