Spectrophotometric Determination of Catecholamines Via Charge Transfer Complexation with Bromanil, Applications to Catecholamine Drug Formulations

  • MAHMOOD A. HASAN University of Duhok
Keywords: The

Abstract

A simple, sensitive, rapid and low-cost spectrophotometric method for catecholamine (levodopa, methyldopa and dopamine) determination in pure and drug formulations is described. This method is based on the complexation reaction of catecholamine with bromanil. Absorbance of the resulting green colored products is measured at 741 nm for levodopa complex and, 738 nm for methyldopa and dopamine. Beer's Law is obeyed in a concentration range of 0.2 – 3.5 µg ml-1 for levodopa, 0.1 – 4.0 µg ml-1 for methyldopa, and 0.2 – 2.5 µg ml-1  for dopamine and the molar absorptivity is 38222, 41474, and 48202 l.mol-1 cm-1 for levodopa, methyldopa, and dopamine respectively,  with an excellent correlation coefficient (r = 0.9974) for levodopa, and (r = 0.998) for both methyldopa and dopamine.. The results show a simple, accurate, fast and readily applied method to the determination of levodopa methyldopa, and dopamine in pharmaceutical products. The analytical results obtained for these products by the proposed method are in agreement with those of the official standard method.   

Author Biography

MAHMOOD A. HASAN, University of Duhok

Dept. of chemistry, Faculty of Science, University of Duhok, Kurdistan Region-Iraq (Accepted for publication: June 9, 2013)

References

Bergamini, M.F., Santos, Stradiotto A. L., Zanoni N. R. 2005, A disposable electrochemical sensor for the determination of levodopa, M. V. B. J. of pharmaceutical and Biomedical analysis Vol. 39, No. 1, pp. 54-59.
British Pharmacopeia 2007.
Canov es Torres. A., mellado Romero A. and
Martinez calatayud. J. 1998, FIAFluorometric determination of adrenaline in pharmaceutical formulations by oxidation with molecular oxygen

Yuanyuan Suna, Yuhai Tanga, Xiaohui Zhenga,
Hong Yaoa & Zhao Xua 2004,
Determination of Catecholamines by Flow Injection Chemiluminescence Method Based on Their Restraining Effects on the
Luminol–Potassium Chlorate System., Analytical Letters, Vol. 37, Issue 12, pp. 2445-2458.
Zapata-Urzùa C, Pérez-Ortiz M, Bravo M, Olivieri AC, Alvarez-Lueje A. 2010. Simultaneous voltammetric determination of levodopa, carbidopa and benserazide in pharmaceuticals using multivariate calibration. Talanta 82 (3), pp. 962-968.
considerations. Analytical Letters, Vol. 39, No. Issue 2, pp. 327-339.
Nagaraja P., Sirinivasa Murthy K. C., et.al. 1998, "Spectrophotometric for the determination of certain catecholamine derivatives in pharmaceutical preparations", Talanta, Vol.46, pp. 39-44.
Paulo Roberto, Ribeiro S., Leonardo Pezza, and Helena R. Pezza. 2006, Determination of methyldopa in pharmaceutical formulations by combined spot testdiffuse reflectance spectroscopy. J. Braz.
Chem. Soc., Vol. 17, No. 4, 674-679.
Published
2013-06-30
How to Cite
HASAN, M. (2013). Spectrophotometric Determination of Catecholamines Via Charge Transfer Complexation with Bromanil, Applications to Catecholamine Drug Formulations. Science Journal of University of Zakho, 1(1), 253-260. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/84
Section
Science Journal of University of Zakho