The Effect of Urtica Dioica Extracts on Third Larval Instars of Tribolium Confusum (DU VAL) Using Two Methods of Extraction

Authors

  • Mehvan M. Ali Rasheed Dept. of Biology, Faculty of Science, University of Zakho, Zakho, Kurdistan Region, Iraq
  • Badia'a Mahmoud Al-Chalabi Dept. of Biology, Faculty of Science, University of Zakho, Zakho, Kurdistan Region, Iraq
  • Sofyan H. Sedo Al-Sinjari Dept. of Biology, Faculty of Science, University of Zakho, Zakho, Kurdistan Region, Iraq

DOI:

https://doi.org/10.25271/sjuoz.2022.10.4.974

Keywords:

Urtica dioica, Tribolium confusum, Extraction methods, mortality rate.

Abstract

The effect of ethanolic extracts of Urtica dioica on mortality of the third Larval instar of Tribolium confusum, was studied using the Soxhlet and solvent extraction methods. Experiments at 30 ± 1°C and 70 ± 5% relative humidity. In laboratory conditions. The mortality data were recorded after 24, 48, and 72 hours. The mortality rate increased by an increase in concentrations (0.5, 1, 1.5, and 2.5 mg/ml), the highest mortality rate was 100% at 1.5 mg/ml, while the lowest percentage of mortality 73.33% was observed after 24 hrs of exposure at 0.5 mg/ml dose by Soxhlet extraction method, whereas the highest mortality rates 100% at 2.5 mg/ml concentration, while the lowest percentage of mortality 90% was observed after 24 hrs of exposure at 0.5 mg/ml dose by Solvent extraction methods. The results show that the plant extracts tested at different concentrations were toxic to the larvae of T. confusum, and both methods of extraction had nearly similar effects on larvae.

 

Author Biographies

Mehvan M. Ali Rasheed, Dept. of Biology, Faculty of Science, University of Zakho, Zakho, Kurdistan Region, Iraq

Dept. of Biology, Faculty of Science, University of Zakho, Zakho, Kurdistan Region, Iraq (Mehvan.ali@stud.uoz.edu.krd)

Badia'a Mahmoud Al-Chalabi , Dept. of Biology, Faculty of Science, University of Zakho, Zakho, Kurdistan Region, Iraq

Dept. of Biology, Faculty of Science, University of Zakho, Zakho, Kurdistan Region, Iraq (badeyamahmoud50@gmail.com)

Sofyan H. Sedo Al-Sinjari , Dept. of Biology, Faculty of Science, University of Zakho, Zakho, Kurdistan Region, Iraq

Dept. of Biology, Faculty of Science, University of Zakho, Zakho, Kurdistan Region, Iraq (sofyan.sedo@uoz.edu.krd)

References

Abbott, W. S. (1925). A method of computing the effectiveness of an insecticide. J. Econ. Entomol, 18(2), 265–267.

Abdulmajeed, A. M., & Al-Chalabi, B. . (2017). The Cumulative Effects of Plant Extract Tavri Shrub (Daphne Mucronata) on Some Biological Aspects of Mosquito Culex Molestus Forskal. Science Journal of University of Zakho, 5(2), 167–171.

AL-Attar, H. J. (2006). Comparison of toxicity and residual effectiveness of Actara®(Thiamethoxam®) with three pyrethroid insecticides against five Coleopteran species infesting stored–products. Tikrit Journal of Pure Science, 11(2).

AL-Jamil, S. K., & AL-Sinjari, S. H. S. (2019). The Effect of the Extent of the Exposure and Concentration for the Three Types of Surface Treatment in the Mortality of Larvae and Adults of Confused Flour Beetle, Tribolum confusum (Du Val). Tikrit Journal for Agricultural Sciences, 18(3), 100–107.

Al-Joary, Y. I. M. A., Al-Obaidi, R. G., & Al-Ejaidy, S. (2021). ‘study of the repellent activity of some medicinal herbs powder against adults of Tribolium confusum duv.(tenebrionidae: coleoptera).’ Plant Archives, 21(1), 445–450.

Al-Sinjari, S. H. S., & Al-Attar, H. J. (2015). Toxic effects of essential oils of Elattaria cardamomum L. and Lambda-Cyhalothrin on Tribolium confusum (Duval). SJUOZ, 3, 15–26.

Al Abadi A. Q. et al. (2008). The attractiveness and repellency effect of some vegetable oils in the adult confused flour beetle.Tribolium confusum duv.(Tenebriondae, Coleoptera)’, journal of techniquse, 21(2). Journal of Techniquse, 21(2).

Ali, W. K., & Mohammed, H. H. (2013). Toxic effect of some plant extracts on the mortality of flour beetle Tribolium confusum (Duval)(Coleoptera: Tenebrionidae). Entomol Ornithol Herpetol, 2(115), 983–2161.

Austin, S. B. (2008). The Paradox of L.,“Stinging Nettle”: A Blessing and a Curse.

Banat, F., Pal, P., Jwaied, N., & Al-Rabadi, A. (2013). Extraction of olive oil from olive cake using soxhlet apparatus. American Journal of Oil and Chemical Technologies, 1(4), 1–8.

Bandar, H., Hijazi, A., Rammal, H., Hachem, A., Saad, Z., & Badran, B. (2013). Techniques for the extraction of bioactive compounds from Lebanese Urtica Dioica. American Journal of Phytomedicine and Clinical Therapeutics, 1(6), 507–513.

Bashir, T. M., & Al-Habib, O. A. M. (2021). The Role of K+, Ca2+ channels and Endothelial Hyperpolarizing Factors in Vasorelaxation Induced by Tribulus terrestris. Technium BioChemMed, 2(1), 94–100.

Bisht, S., Bhandari, S., & Bisht, N. S. (2012). Urtica dioica (L): an undervalued, economically important plant. Agric Sci Res J, 2(5), 250–252.

Bourgeois, C., Leclerc, É. A., Corbin, C., Doussot, J., Serrano, V., Vanier, J.-R., Seigneuret, J.-M., Auguin, D., Pichon, C., & Lainé, É. (2016). Nettle (Urtica dioica L.) as a source of antioxidant and anti-aging phytochemicals for cosmetic applications. Comptes Rendus Chimie, 19(9), 1090–1100.

Boussaada, O., Kamel, M. B. H., Ammar, S., Haouas, D., Mighri, Z., & Helal, A. N. (2008). Insecticidal activity of some Asteraceae plant extracts against Tribolium confusum. Bulletin of Insectology, 61(2), 283–289.

Coile, N. C. (1999). Urtica chamaedryoides Pursh: a stinging nettle, or fireweed and some related species. Fla. Department Agric. & Consumer Services, Division of Plant Industry.

Dadang, E. D. F., & Prijono, D. (2009). Effectiveness of two botanical insecticide formulations to two major cabbage insect pests on field application. J. ISSAAS, 15(1), 42–51.

Derbalah, A., & Ahmed, S. (2011). Oil and powder of spearmint as an alternative to Sitophilus oryzae chemical control of wheat grains. Journal of Plant Protection Research.

Esmaili, M., Vojoudi, S., & Parsaeyan, E. (2013). Fumigant toxicity of essential oils of Mentha pulegium L. on adults of Callosobruchus maculatus, Tribolium castaneum, Lasioderma serricorne and Sitophilus oryzae in laboratory conditions. Technical Journal of Engineering and Applied Sciences, Giza, 3(9), 732–735.

Fathi, A., & Shakarami, J. (2014). Larvicidal effects of essential oils of five species of Eucalyptus against Tribolium confusum (du Val) and T. castaneum (Herbest). International Journal of Agriculture and Crop Sciences (IJACS), 7(5), 220–224.

García, M., Donadel, O. J., Ardanaz, C. E., Tonn, C. E., & Sosa, M. E. (2005). Toxic and repellent effects of Baccharis salicifolia essential oil on Tribolium castaneum. Pest Management Science: Formerly Pesticide Science, 61(6), 612–618.

James, D. G., Lauby, G., Seymour, L., & Buckley, K. (2015). Beneficial insects associated with stinging nettle, Urtica dioica Linnaeus, in central Washington State. The Pan-Pacific Entomologist, 91(1), 82–90.

Kareru, P., Kipkorir, Z. R., & Wamaitha, M. E. (2013). Use of botanicals and safer insecticides designed in controlling insects: the African case. Insecticides–Development of Safer and More Effective Technologies, 10, 297–309.

Khanam, L. A. M., Talukder, D., Khan, A. R., & Rahman, S. M. (1990). Insectidical properties of Royna, Aphanamixis polystachya Wall.(Parker)(Meliaceae) against Tribolium confusum Duval. Journal of Asiatic Society of Bangladesh Science, 16, 71–74.

Khoshnoud, H., & Khayamy, M. (2008). Insecticidal effects of ethanolic extract from Verbascum cheiranthifolium Boiss. against two stored-product insect pests species. Journal of Biological Sciences, 8(1), 191–195.

Kregiel, D., Pawlikowska, E., & Antolak, H. (2018). Urtica spp.: Ordinary plants with extraordinary properties. Molecules, 23(7), 1664.

Lee, B.-H., Annis, P. C., & Choi, W.-S. (2004). Fumigant toxicity of essential oils from the Myrtaceae family and 1, 8-cineole against 3 major stored-grain insects. Journal of Stored Products Research, 40(5), 553–564.

Liu, Z. L., Zhao, N. N., Liu, C. M., Zhou, L., & Du, S. S. (2012). Identification of insecticidal constituents of the essential oil of Curcuma wenyujin rhizomes active against Liposcelis bostrychophila Badonnel. Molecules, 17(10), 12049–12060.

Mahfuz, I., & Khanam, L. A. M. (2007). Toxicity of some indigenous plant extracts against Tribolium confusum Duval. Journal of Bio-Science, 15, 133–138.

Mohammed, H. H. (2013). Repellency of Ethanolic Extract of Some Indigenous Plants against Tribolium confusum(Duval)(Coleoptera: Tenebrionidae). Agric. Vet. Sci, 2(6), 27–31.

Ndione, R. D., Faye, O., Ndiaye, M., Dieye, A., & Afoutou, J. M. (2007). Toxic effects of neem products (Azadirachta indica A. Juss) on Aedes aegypti Linnaeus 1762 larvae. African Journal of Biotechnology, 6(24).

Ngamo, T. S., Ngatanko, I., Ngassoum, M. B., Mapongmestsem, P. M., & Hance, T. (2007). Persistence of insecticidal activities of crude essential oils of three aromatic plants towards four major stored product insect pests. African Journal of Agricultural Research, 2(4), 173–177.

Phillips, T. W., & Throne, J. E. (2010). Biorational approaches to managing stored-product insects. Annual Review of Entomology, 55, 375–397.

Raja, M., & William, S. J. (2008). Impact of volatile oils of plants against the cowpea beetle Callosobruchus maculatus (FAB.)(Coleoptera: Bruchidae). International Journal of Integrative Biology, 2(1), 62–64.

Saidana, D., Halima-Kamel, M. Ben, Mahjoub, M. A., Haouas, D., Mighri, Z., & Helal, A. N. (2007). Insecticidal activities of Tunisian halophytic plant extracts against larvae and adults of Tribolium confusum. Tropicultura, 25(4), 193.

Saleem, M., Hussain, D., Rashid, R. S., Saleem, H. M., Ghouse, G. H., & Abbas, M. (2013). Insecticidal activities of two citrus oils against Tribolium castaneum (herbst). American Journal of Research Communication, 1(6), 67–74.

Scientific, A. (2018). Solvent extraction method of plant using ethanol. coleparmer.co.uk/blog/2018/06/28/steps-safe-solvent-extraction/

Verheggen, F., Ryne, C., Olsson, P.-O., Arnaud, L., Lognay, G., Högberg, H.-E., Persson, D., Haubruge, E., & Löfstedt, C. (2007). Electrophysiological and behavioral activity of secondary metabolites in the confused flour beetle, Tribolium confusum. Journal of Chemical Ecology, 33(3), 525–539.

Zaka, S. M., Iqbal, N., Saeed, Q., Akrem, A., Batool, M., Khan, A. A., Anwar, A., Bibi, M., Azeem, S., & Bibi, R. (2019). Toxic effects of some insecticides, herbicides, and plant essential oils against Tribolium confusum Jacquelin du Val (Insecta: Coleoptera: Tenebrionidae). Saudi Journal of Biological Sciences, 26(7), 1767–1771.

Zhou, S., Chan, S. Y., Goh, B. C., Chan, E., Duan, W., Huang, M., & McLeod, H. L. (2005). Mechanism-based inhibition of cytochrome P450 3A4 by therapeutic drugs. Clinical Pharmacokinetics, 44(3), 279–304.

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Published

2022-11-07

How to Cite

Rasheed, M. M. A., Al-Chalabi , B. M., & Al-Sinjari , S. H. S. (2022). The Effect of Urtica Dioica Extracts on Third Larval Instars of Tribolium Confusum (DU VAL) Using Two Methods of Extraction. Science Journal of University of Zakho, 10(4), 201–205. https://doi.org/10.25271/sjuoz.2022.10.4.974

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Science Journal of University of Zakho