Vasorelaxant and Antispasmodic Effects of Some Polyphenols and Glycoside in Rat Smooth Muscles
Keywords:
vasorelaxant, antispasmodic, polyphenol, glycoside, rat, smooth musclesAbstract
Objective: The present study was undertaken to investigate the effect of Luteolin, chlorogenic acid, amygdalin and tannic acid on vascular smooth muscle contractility.
Materials and Methods: The effect of different concentrations of some polyphenols and glycosides on contractile responses of isolated aorta, ileum and trachea to Acetylcholine, KCl and phenylephrine (PE) were evaluated.
Results: The results of the current study indicated that luteolin and chlorogenic acid has potent relaxant effects on KCl and PE-induced contractions in rat’s aortic ring. Amygdalin and tannic acid reduced ACh-induced ileum contractions significantly. Conclusions: The inhibitory effect of luteolin and chlorogenic acid on the contraction induced by PE and KCl may be due to their anti-adrenergic and anti-hyperpolarizing effect.
References
Andrade R.G., Jr., Dalvi L.T., Silva J.M., Jr., Lopes G.K., Alonso A. & Hermes-Lima M. (2005) The antioxidant effect of tannic acid on the in vitro copper-mediated formation of free radicals. Arch Biochem Biophys 437, 1-9.
Bankar G.R., Nayak P.G., Bansal P., Paul P., Pai K.S., Singla R.K. & Bhat V.G. (2011) Vasorelaxant and antihypertensive effect of Cocos nucifera Linn. endocarp on isolated rat thoracic aorta and DOCA salt-induced hypertensive rats. J References
Andrade R.G., Jr., Dalvi L.T., Silva J.M., Jr., Lopes G.K., Alonso A. & Hermes-Lima M. (2005) The antioxidant effect of tannic acid on the in vitro copper-mediated formation of free radicals. Arch Biochem Biophys 437, 1-9.
Bankar G.R., Nayak P.G., Bansal P., Paul P., Pai K.S., Singla R.K. & Bhat V.G. (2011) Vasorelaxant and antihypertensive effect of Cocos nucifera Linn. endocarp on isolated rat thoracic aorta and DOCA salt-induced hypertensive rats. J Ethnopharmacol 134, 50-4.
Baroni A., Paoletti I., Greco R., Satriano R.A., Ruocco E., Tufano M.A. & Perez J.J. (2005) Immunomodulatory effects of a set of amygdalin analogues on human keratinocyte cells. Experimental Dermatology 14, 854-9.
Calixto J.B., Nicolau M. & Rae G.A. (1986) Pharmacological actions of tannic acid. I. Effects on isolated smooth and cardiac muscles and on blood pressure. Planta Med, 32-5.
Chang K.H., Shin M.-S., Yang H.-Y., Lee J.-W., Kim Y.-S., Lee M.-H., Kim J., Kim K.-H. & Kim C.-J. (2006) Amygdalin induces apoptosis through regulation of Bax and Bcl-2 expressions in human DU145 and LNCaP prostate cancer cells. Biol. Pharm. Bull. 29, 1597– 602.
Chen D., Bi A., Dong X., Jiang Y., Rui B., Liu J., Yin Z. & Luo L. (2014) Luteolin exhibits anti-inflammatory effects by blocking the activity of heat shock protein 90 in macrophages. Biochem Biophys Res Commun 443, 326-32.
Chung K.F. (2008) Airway Smooth Muscle in Asthma and COPD: Biology and Pharmacology. In: Airway Smooth Muscle in Asthma and COPD (pp. 303-12. John Wiley & Sons, Ltd.
Duarte J., Perez Vizcaino F., Utrilla P., Jimenez J., Tamargo J. & Zarzuelo A. (1993) Vasodilatory effects of flavonoids in rat aortic smooth muscle. Structure-activity relationships. Gen Pharmacol 24, 857-62.
Fatehi M., Farifteh F. & Fatehi-Hassanabad Z. (2004) Antispasmodic and hypotensive effects of Ferula asafoetida gum extract. J Ethnopharmacol 91, 321-4.
Fulton D., Mcgiff J.C. & Quilley J. (1996) Cytochrome P450 arachidonate metabolites: deficit in diabetes mellitus. FASEB J 9.
Graf B.A., Milbury P.E. & Blumberg J.B. (2005) Flavonols, flavones, flavanones, and human health: epidemiological evidence. J Med Food 8, 281-90.
Gülçin İ., Huyut Z., Elmastaş M. & Aboul-Enein H.Y. (2010) Radical scavenging and antioxidant activity of tannic acid. Arabian Journal of Chemistry 3, 43-53.
Habauzit V. & Morand C. (2012) Evidence for a protective effect of polyphenols-containing foods on cardiovascular health: an update for clinicians. Therapeutic Advances in Chronic Disease 3, 87-106.
Hwang H.J., Kim P., Kim C.J., Lee H.J., Shim I., Yin C.S., Yang Y. & Hahm D.H. (2008) Antinociceptive effect of amygdalin isolated from Prunus armeniaca on formalin-induced pain in rats. Biol Pharm Bull 31, 1559-64.
Hwang S.J., Kim Y.W., Park Y., Lee H.J. & Kim K.W. (2014) Anti-inflammatory effects of chlorogenic acid in lipopolysaccharide-stimulated RAW 264.7 cells. Inflamm Res 63, 81-90.
Karaki H. & Weiss G.B. (1988) Calcium release in smooth muscle. Life Sci 42, 111-22.
Ko W.C., Shih C.M., Leu I.J., Chen T.T. & Chang J.P. (2005) Mechanisms of relaxant action of luteolin in isolated guinea pig trachea. Planta Med 71, 406-11.
Ethnopharmacol 134, 50-4.
Baroni A., Paoletti I., Greco R., Satriano R.A., Ruocco E., Tufano M.A. & Perez J.J. (2005) Immunomodulatory effects of a set of amygdalin analogues on human keratinocyte cells. Experimental Dermatology 14, 854-9.
Calixto J.B., Nicolau M. & Rae G.A. (1986) Pharmacological actions of tannic acid. I. Effects on isolated smooth and cardiac muscles and on blood pressure. Planta Med, 32-5.
Kumar S.P., Patel J.S. & Saraf M.N. (2008) Mechanism of vasorelaxant activity of a fraction of root extract of Sesamum indicum Linn. Indian J Exp Biol 46, 457-64.
Leblais V., Krisa S., Valls J., Courtois A., Abdelouhab S., Vila A.M., Merillon J.M. & Muller B. (2008) Relaxation induced by red wine polyphenolic compounds in rat pulmonary arteries: lack of inhibition by NO-synthase inhibitor. Fundam Clin Pharmacol 22, 25-35.
Loizzo M.R., Said A., Tundis R., Rashed K., Statti G.A., Hufner A. & Menichini F. (2007) Inhibition of angiotensin converting enzyme (ACE) by flavonoids isolated from Ailanthus excelsa (Roxb) (Simaroubaceae). Phytother Res 21, 32-6.
Menaa F., Menaa A. & Tréton J. (2014) Polyphenols in Human Health and Disease. 1, 819–30.
Namkung W., Thiagarajah J.R., Phuan P.-W. & Verkman A.S. (2010) Inhibition of Ca(2+)-activated Cl(−) channels by gallotannins as a possible molecular basis for health benefits of red wine and green tea. The FASEB Journal 24, 4178-86.
Reddy H., Watson N., Ford A.P.D.W. & Eglen R.M. (1995) Characterization of the interaction between muscarinic M2 receptors and β-adrenoceptor subtypes in guinea-pig isolated ileum. Br J Pharmacol 114, 49-56.
Sato Y., Itagaki S., Kurokawa T., Ogura J., Kobayashi M., Hirano T., Sugawara M. & Iseki K. (2011) In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid. Int J Pharm 403, 136-8.
Shekha M.S. & Al-Habib O.A.M. (2012) Vasorelaxant, antispasmodic and Cardiotonic effect of the Chloroform Fraction of Crataegus aronia on isolated rat muscles. International Journal of Biological and Biomedical Sciences 1, 6-11.
Shi T., Duan Z.H., Papay R., Pluskota E., Gaivin R.J., de la Motte C.A., Plow E.F. & Perez D.M. (2006) Novel alpha1-adrenergic receptor signaling pathways: secreted factors and interactions with the extracellular matrix. Mol Pharmacol 70, 129-42.
Song Y.S. & Park C.M. (2014) Luteolin and luteolin-7-O-glucoside strengthen antioxidative potential through the modulation of Nrf2/MAPK mediated HO-1 signaling cascade in RAW 264.7 cells. Food Chem Toxicol 65, 70-5.
Zhao Y., Wang J., Ballevre O., Luo H. & Zhang W. (2012) Antihypertensive effects and mechanisms of chlorogenic acids. Hypertens Res 35, 370-4
Bankar G.R., Nayak P.G., Bansal P., Paul P., Pai K.S., Singla R.K. & Bhat V.G. (2011) Vasorelaxant and antihypertensive effect of Cocos nucifera Linn. endocarp on isolated rat thoracic aorta and DOCA salt-induced hypertensive rats. J References
Andrade R.G., Jr., Dalvi L.T., Silva J.M., Jr., Lopes G.K., Alonso A. & Hermes-Lima M. (2005) The antioxidant effect of tannic acid on the in vitro copper-mediated formation of free radicals. Arch Biochem Biophys 437, 1-9.
Bankar G.R., Nayak P.G., Bansal P., Paul P., Pai K.S., Singla R.K. & Bhat V.G. (2011) Vasorelaxant and antihypertensive effect of Cocos nucifera Linn. endocarp on isolated rat thoracic aorta and DOCA salt-induced hypertensive rats. J Ethnopharmacol 134, 50-4.
Baroni A., Paoletti I., Greco R., Satriano R.A., Ruocco E., Tufano M.A. & Perez J.J. (2005) Immunomodulatory effects of a set of amygdalin analogues on human keratinocyte cells. Experimental Dermatology 14, 854-9.
Calixto J.B., Nicolau M. & Rae G.A. (1986) Pharmacological actions of tannic acid. I. Effects on isolated smooth and cardiac muscles and on blood pressure. Planta Med, 32-5.
Chang K.H., Shin M.-S., Yang H.-Y., Lee J.-W., Kim Y.-S., Lee M.-H., Kim J., Kim K.-H. & Kim C.-J. (2006) Amygdalin induces apoptosis through regulation of Bax and Bcl-2 expressions in human DU145 and LNCaP prostate cancer cells. Biol. Pharm. Bull. 29, 1597– 602.
Chen D., Bi A., Dong X., Jiang Y., Rui B., Liu J., Yin Z. & Luo L. (2014) Luteolin exhibits anti-inflammatory effects by blocking the activity of heat shock protein 90 in macrophages. Biochem Biophys Res Commun 443, 326-32.
Chung K.F. (2008) Airway Smooth Muscle in Asthma and COPD: Biology and Pharmacology. In: Airway Smooth Muscle in Asthma and COPD (pp. 303-12. John Wiley & Sons, Ltd.
Duarte J., Perez Vizcaino F., Utrilla P., Jimenez J., Tamargo J. & Zarzuelo A. (1993) Vasodilatory effects of flavonoids in rat aortic smooth muscle. Structure-activity relationships. Gen Pharmacol 24, 857-62.
Fatehi M., Farifteh F. & Fatehi-Hassanabad Z. (2004) Antispasmodic and hypotensive effects of Ferula asafoetida gum extract. J Ethnopharmacol 91, 321-4.
Fulton D., Mcgiff J.C. & Quilley J. (1996) Cytochrome P450 arachidonate metabolites: deficit in diabetes mellitus. FASEB J 9.
Graf B.A., Milbury P.E. & Blumberg J.B. (2005) Flavonols, flavones, flavanones, and human health: epidemiological evidence. J Med Food 8, 281-90.
Gülçin İ., Huyut Z., Elmastaş M. & Aboul-Enein H.Y. (2010) Radical scavenging and antioxidant activity of tannic acid. Arabian Journal of Chemistry 3, 43-53.
Habauzit V. & Morand C. (2012) Evidence for a protective effect of polyphenols-containing foods on cardiovascular health: an update for clinicians. Therapeutic Advances in Chronic Disease 3, 87-106.
Hwang H.J., Kim P., Kim C.J., Lee H.J., Shim I., Yin C.S., Yang Y. & Hahm D.H. (2008) Antinociceptive effect of amygdalin isolated from Prunus armeniaca on formalin-induced pain in rats. Biol Pharm Bull 31, 1559-64.
Hwang S.J., Kim Y.W., Park Y., Lee H.J. & Kim K.W. (2014) Anti-inflammatory effects of chlorogenic acid in lipopolysaccharide-stimulated RAW 264.7 cells. Inflamm Res 63, 81-90.
Karaki H. & Weiss G.B. (1988) Calcium release in smooth muscle. Life Sci 42, 111-22.
Ko W.C., Shih C.M., Leu I.J., Chen T.T. & Chang J.P. (2005) Mechanisms of relaxant action of luteolin in isolated guinea pig trachea. Planta Med 71, 406-11.
Ethnopharmacol 134, 50-4.
Baroni A., Paoletti I., Greco R., Satriano R.A., Ruocco E., Tufano M.A. & Perez J.J. (2005) Immunomodulatory effects of a set of amygdalin analogues on human keratinocyte cells. Experimental Dermatology 14, 854-9.
Calixto J.B., Nicolau M. & Rae G.A. (1986) Pharmacological actions of tannic acid. I. Effects on isolated smooth and cardiac muscles and on blood pressure. Planta Med, 32-5.
Kumar S.P., Patel J.S. & Saraf M.N. (2008) Mechanism of vasorelaxant activity of a fraction of root extract of Sesamum indicum Linn. Indian J Exp Biol 46, 457-64.
Leblais V., Krisa S., Valls J., Courtois A., Abdelouhab S., Vila A.M., Merillon J.M. & Muller B. (2008) Relaxation induced by red wine polyphenolic compounds in rat pulmonary arteries: lack of inhibition by NO-synthase inhibitor. Fundam Clin Pharmacol 22, 25-35.
Loizzo M.R., Said A., Tundis R., Rashed K., Statti G.A., Hufner A. & Menichini F. (2007) Inhibition of angiotensin converting enzyme (ACE) by flavonoids isolated from Ailanthus excelsa (Roxb) (Simaroubaceae). Phytother Res 21, 32-6.
Menaa F., Menaa A. & Tréton J. (2014) Polyphenols in Human Health and Disease. 1, 819–30.
Namkung W., Thiagarajah J.R., Phuan P.-W. & Verkman A.S. (2010) Inhibition of Ca(2+)-activated Cl(−) channels by gallotannins as a possible molecular basis for health benefits of red wine and green tea. The FASEB Journal 24, 4178-86.
Reddy H., Watson N., Ford A.P.D.W. & Eglen R.M. (1995) Characterization of the interaction between muscarinic M2 receptors and β-adrenoceptor subtypes in guinea-pig isolated ileum. Br J Pharmacol 114, 49-56.
Sato Y., Itagaki S., Kurokawa T., Ogura J., Kobayashi M., Hirano T., Sugawara M. & Iseki K. (2011) In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid. Int J Pharm 403, 136-8.
Shekha M.S. & Al-Habib O.A.M. (2012) Vasorelaxant, antispasmodic and Cardiotonic effect of the Chloroform Fraction of Crataegus aronia on isolated rat muscles. International Journal of Biological and Biomedical Sciences 1, 6-11.
Shi T., Duan Z.H., Papay R., Pluskota E., Gaivin R.J., de la Motte C.A., Plow E.F. & Perez D.M. (2006) Novel alpha1-adrenergic receptor signaling pathways: secreted factors and interactions with the extracellular matrix. Mol Pharmacol 70, 129-42.
Song Y.S. & Park C.M. (2014) Luteolin and luteolin-7-O-glucoside strengthen antioxidative potential through the modulation of Nrf2/MAPK mediated HO-1 signaling cascade in RAW 264.7 cells. Food Chem Toxicol 65, 70-5.
Zhao Y., Wang J., Ballevre O., Luo H. & Zhang W. (2012) Antihypertensive effects and mechanisms of chlorogenic acids. Hypertens Res 35, 370-4
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2014-12-30
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Shekha, M. S. (2014). Vasorelaxant and Antispasmodic Effects of Some Polyphenols and Glycoside in Rat Smooth Muscles. Science Journal of University of Zakho, 2(2), 220–227. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/177
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