Relaxant Effects of Rutin on Rat Smooth Muscles

  • Mudhir S. Shekha University of Salahaddin
  • Omar A.M Al-Habib University of Zakho
Keywords: relaxant, aorta, Ileum, Trachea, Rutin

Abstract

The aim of the present study was to investigate the relaxant effects of rutin on rat thoracic aorta, ileum and trachea. Rutin (1X10-7 - 6X10-3 M) caused a concentration-dependent relaxation of aortic rings precontracted with phenylephrine (PE 10-6M) while produced non-significant relaxed effect on KCl induced contraction with Log IC50’s -3.025 and -2.885 M/ml respectively. Rutin exhibited potent inhibitory effects on (ACh  10-6 M) precontracted ileum of the rat with Log IC50 -5.996 M/mL. Rutin produced more potent inhibitory effect on ACh- than KCl-induced contractions in trachea, with Log IC50’s of -4.371 and -4.301 M/mL respectively. While rutin showed more potent inhibitory effect on PE-induced contractions potently than KCl precontracted aorta with Log IC50’s was -3.025 M and  -2.885 respectively. From the data obtained during the present work, it can be concluded that rutin induces concentration-dependent relaxation in rat's smooth muscle. The mechanism of the relaxation may involves via nitric oxide-guanylyl cyclase pathway  for aorta and anticholinergic activity for ileum and trachea.

Author Biographies

Mudhir S. Shekha, University of Salahaddin

Dept. of Biology, Colleges of Science, University of Salahaddin, Kurdistan Region-

Omar A.M Al-Habib, University of Zakho

Dept. of Biology, Faculty of Science, University of Zakho, Kurdistan Region-Iraq

References

Amira S, Rotondo A, Mulè F. 2008. Relaxant effects of flavonoids on the mouse isolated stomach: structure-activity relationships. Eur J Pharmacol. 3;599 (1-3):126-30.
Arts I. C. and Hollman P. C. 2005 “Polyphenols and Disease Risk in Epidemiologic Studies,” The American Journal of Clinical Nutrition, Vol. 81, No. 1, 317S-325S.
Petri, N., Tannergren, C., Holst, B., Mellon, F., Bao, Y., Plumb, G., Bacon, J., O'Leary, K., Kroon, P., Knutson, L., Forsell, P., Eriksson, T., Lennernas, H., & Williamson, G. (2003). Absorption/ metabolism of sulforaphane and quercetin, and regulation of phase ii enzymes, in human jejunum in vivo. Drug Metabolism and Disposition 31: 805-813.
Djelili H. , Lekhmici A, Emmanuel N, and Philippe D. (2012). Relaxant Effects of Quercetin and Rutin on Human Isolated Bronchus. Chinese Medicine, 3, 94-100
Macander P. J. 1986. “Flavonoids Affect Acetylcholine, Pros-taglandin E2 and Antigen-Mediated Smooth Muscle Con-traction,” Progress in Clinical and Biological Research, Vol. 213, pp. 489-492.
Van Rossum, J.M., 1963. Cumulative concentration response curves. II. Techniques for the making of concentration–response curves in isolated organs and the evaluation of drug parameters. Archives Internationales de Pharmacodynamie et de Therapie 143, 299–330
Fulton D., Mcgiff J.C. and Quilley J. 1996. Cytochrome P450 arachidonate metabolites: deficit in diabetes mellitus. FASEB J.;9:A113.
Zhou XM, Yao H, Xia ML, Cao CM, Jiang HD and Xia Q. 2006. Comparison of vasodilatation effect between quercetin and rutin in the isolated rat thoracic aorta. Zhejiang Da Xue Xue Bao Yi Xue Ban. Jan;35(1):29-33
Abdalla S, Zarga MA, Afifi F, al-Khalil S, Mahasneh A, Sabri S. 1989. Effects of 3,3'-di-O-methylquercetin on guinea-pig isolated smooth muscle. J Pharm Pharmacol. 41(2):138-41.
Gojković-Bukarica L , Novaković A , Lešić A. , Bumbaširević M. . 2007. Endothelium-dependent relaxation of rat aorta induced by resveratrol Acta Veternaria (Beograd) Vol. 57, 2-3. 123-132 .
Published
2013-09-30
How to Cite
Shekha, M., & Al-Habib, O. (2013). Relaxant Effects of Rutin on Rat Smooth Muscles. Science Journal of University of Zakho, 1(2), 543-548. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/264
Section
Science Journal of University of Zakho