Effects of Punica Granatum Seed Hydromethanol Extract on Contractility of Isolated Aorta in Female Albino Rats

  • Omar A. M. Al-Habib University of Zakho
  • Lina N. Adam University of Zakho
Keywords: Punica granatum, hydro methanol seeds extract, K -channels blockers, Ca -channels blocker, L-NAME, COX


The aim of the present study was to evaluate the physiological effect of the Punica granatum hydromethanol seeds extract on the contractility of isolated rat aortic rings. The isolated rat’s thoracic aorta was placed in an organ bath containing Krebs solution and the contraction was recorded isometrically. The results demonstrated that P. granatum hydro- methanol extract (1.5 to 5 mg/ml) caused a significant relaxant effect on the contractions induced by phenylephrine (0.01 mM) with IC50 ± SEM of 2.682 ± 0.197 mg/ml and the percentage of relaxation for PE-induced contraction was 52.88 ± 0.831. In endothelium intact and denuded aortic rings, the extract induced more or less the same response, except at high concentrations used (4.5 – 5.0 mg/ml) in which the endothelium denuded rings produced a significant reduction in the percent of relaxation from 49.91 to 25.42.  Relaxant effect of hydromethanol seed extracts on intact aorta was not affected by nitric oxide synthase sinhsibitor ( L-NAME, 3*10-4), gaunyl cyclase inhibitor ( methylene blue 1*10-5) and PGI2 inhibitor (Indomethacin, 3*10-5), and thus, the percentages of relaxation were 59.85 ± 0.084, 58.59 ± 0.566 and 56.76 ± 0.693 respectively. In addition, incubation of aortic rings with the K+ channels blockers TEA, GLIB,, 4AP and BaCl2, that Kca, KATP and Kv channels played no role on vasorelaxation induced by hydromethanol extract, while Kir enhanced the relaxation induced by the extract to76.40%. Finally, hydro methanol extract significantly enhanced dose-response relaxation after incubation of thoracic aortic rings with Nifedipine (10-6 M) to 91.91 % with IC50 ± SEM 1.774 ± 0.096. It can be concluded from the results of the current work that the fraction of Punica granatum hydromethanol seed extract has vasorelaxant effects on rat aortic which was partially dependent on endothelium, Kir and Ca channels.

Author Biographies

Omar A. M. Al-Habib, University of Zakho

Dept. of Biology, Faculty of Science, University of Zakho, Kurdistan Region, Iraq. (omar.alhabib@uoz.edu.krd)

Lina N. Adam, University of Zakho

Dept. of Biology, Faculty of Science, University of Zakho, Kurdistan Region, Iraq. (lina.adam@uoz.edu.krd)


Ajay, M., Gilani A. H. and Mustafa M. R., (2003). Effects of flavonoids on vascular smooth muscle of the isolated rat thoracic aorta. Life Sciences. 74(5): 603-612.
Al-Habib, O. A., Salihi A. B. Q., Moran O., Picco C. and Baroni D., (2015). Modulation of Aortic Inward Rectifier Potassium Channels Activity by Hydrogen Sulfide. Conference Paper.
AL-Habib, O.A.M. and Shekha M.S., (2010). Vasorelaxant Effect of Aqueous Extract of Crataegus azarolus aronia and Quercetin on Isolated Albino Rat’s Thoracic Aorta. J. Duhok Univ. 13 (1): 7-13.
Amin, H., Ibrahim M. F., Hussain F., Sardar A. and Vidari, G., (2016). Phytochemistry and Ethnopharmacology of Some Medicinal Plants Used in the Kurdistan Region of Iraq. Natural product communications. 11(3): 291-296.
Anahita, A., Asmah R. and Fauziah O., (2015). Evaluation of total phenolic content, total antioxidant activity, and antioxidant vitamin composition of pomegranate seed and juice. General Medicine: Open Access, 2015.
Borochov-Neori, H., Judeinstein S., Tripler E., Harari M., Greenberg A., Shomer I. and Holland D., (2009). Seasonal and cultivar variations in antioxidant and sensory quality of pomegranate (Punica granatum L.) fruit. Journal of Food Composition and Analysis. 22(3): 189-195.
Costantini, S., Rusolo F., De Vito V., Moccia S., Picariello G., Capone F., Guerriero E., Castello G., Volpe, M. G., (2014). Potential anti-inflammatory effects of the hydrophilic fraction of pomegranate (Punica granatum L.) seed oil on breast cancer cell lines. Molecules. 19(6): 8644-8660.
Dipak, G., (2012). Phytochemical and pharmacological profile of Punica granatum. International Journal of Pharmacy. 3(2): 65-68.
Fulton, D., McGiff J. C. and Quilley J., (1996). Role of phospholipase C and phospholipase A2 in the nitric oxide-independent vasodilator effect of bradykinin in the rat perfused heart. Journal of Pharmacology and Experimental Therapeutics. 278(2): 518-526.
Herlekar, I., (2014). Fruit power: pomegranate helps fight Hepatitis C infection. CURRENT SCIENCE. 107(7): 1105.
Nakamura, Y., Matsumoto H. and Todoki K., (2002). Endothelium-dependent vasorelaxation induced by black currant concentrate in rat thoracic aorta. The Japanese journal of pharmacology. 89(1): 29-35.
Nostro, A., Germano M., D’angelo V., Marino A. and Cannatelli M., (2000). Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Letters in applied microbiology. 30(5): 379-384.
Salleh, N. and Ahmad V. N., (2013). In-Vitro effect of Ficus deltoidea on the contraction of isolated rat’s uteri is mediated via multiple receptors binding and is dependent on extracellular calcium. BMC complementary and alternative medicine. 13(1):1.
Shahbaz, S.E., (2010). Trees and Shrubs, A Field Guide to the Trees and Shrubs of Kurdistan Region of Iraq. Duhok University Press.
Shekha, M.S.S., (2010). Physiological Effects of Carataegus aronia Fractions on Isolated Smooth Muscle and Perfused “Langendorff” Heart in Albino Rats. Ph.D. thesis. Duhok University. Kurdistan region-Iraq.
Tzulker, R., Glazer I., Bar-Ilan I., Holland D., Aviram M. and Amir R., (2007). Antioxidant activity, polyphenol content, and related compounds in different fruit juices and homogenates prepared from 29 different pomegranate accessions. Journal of Agricultural and Food Chemistry. 55(23): 9559-9570.
Verma, S. and Singh, S., (2008). Current and future status of herbal medicines. Veterinary world. 1(11): 347-350.
Viuda-Martos, M., Ruiz-Navajas Y., Fernández-López J., Sendra E., Sayas-Barberá E. and Pérez-Álvarez J. A., (2011). Antioxidant properties of pomegranate (Punica granatum L.) bagasses obtained as co-product in the juice extraction. Food Research International. 44(5): 1217-1223.
Woods, M. B. and Woods, M., (2011). Ancient medical technology: from herbs to scalpels: Twenty-First Century Books.
How to Cite
Al-Habib, O., & Adam, L. (2017). Effects of Punica Granatum Seed Hydromethanol Extract on Contractility of Isolated Aorta in Female Albino Rats. Science Journal of University of Zakho, 5(1), 37-43. https://doi.org/10.25271/2017.5.1.298
Science Journal of University of Zakho