Relaxant Effects of Essential Oils Of Eucalyptus Camaldulensis on Aortic Rings in Male Albino Rats
Keywords:
Eucalyptus camaldulensis, Eucalyptus camaldulensis, essential oils, vasorelaxationAbstract
The essential oil (EO) produced a potent dilation in aortic rings with percentage of relaxation 95.659%.The significant relaxant effect on thoracic aortic rings was inhibited by GLIB (10-5) and 4-AP (1mM)with percentages of relaxation, 74.577% and 51.494%, respectively. On the other hand, vasorelaxation in aortic rings did not influenced by pretreatment with TEA and BaCl2. Pretreatment of aortic rings with nifedipine, significantly blocked the relaxation to 38.7%. On the other hand, the dose-response shifted to the right in endothelium denuded preparation. The percentage of relaxation in endothelium-denuded was 82.28%. Preincubation of aortic rings with L-NAME and Indomethacin altered the dilation from control significantly, and the percentage of relaxation found to be 60.18% and 77.077%, respectively. The present study suggest that the EO of E .camaldulensis depresses the aortic force development, probably acting as a Ca++ channel antagonism and partially via NO and cyclooxygenase pathways, the involvement of KATP and KV channels in the vasorelaxatoin induced by EO.
References
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Peixoto-Neves, D., Silva-Alves, K.S., Gomes, M.D.M., Lima, F.C., Lahlou, S., Magalhães, P.J.C., Ceccatto, V.M., Coelho-de-Souza, A.N. and Leal-Cardoso, J.H. (2010).Vasorelaxant effects of the monoterpenic phenol isomers, carvacrol and thymol, on rat isolated aorta. Fundamental & Clinical Pharmacology, 24: 341–350.
Perez-Hernandeza, N, Ponce-Montera H, Ortiza MI, Carino-Cortesa R and Joseph-Nathanb P. (2009).Structure-Activity Relationships of Aromadendranes in Uterus-Relaxant Activity. Z. Naturforsch. 64 c: 840 – 846.
Pinto, NV, Assreuy AM, Coelho-de-Souza AN, Ceccatto VM, Magalhães PJ, Lahlou S, Leal-Cardoso JH. (2009). Endothelium-dependent vasorelaxant effects of the essential oil from aerial parts of Alpiniazerumbet and its main constituent 1,8-cineole in rats.
Ribeiro TP, Porto DL, Menezes CP, Antunes AA, Silva DF, De Sousa DP, Nakao LS, Braga VA, Medeiros IA. (2010). Unravelling the cardiovascular effects induced by alpha-terpineol: a role for the nitric oxide-cGMP pathway. ClinExpPharmacol Physiol. 37(8):811-6.
Saito K, Okabe T, Inamori Y, Tsujibo H, Miyake Y, Hiraoka K, Ishida N (1996). The biological properties of monoterpenes: Hypotensive effects on rats and antifungal activities on plant pathogenic fungi of monoterpenes. MokuzaiGakkaishi 42: 677-680.
Santos, MRV, Moreira FV, Fraga BP, De Sousa DP, Bonjardim LR and Quintans-Junior LJ.( 2011). Cardiovascular effects of monoterpenes: a review. Brazilian J Pharmacognosy 21(4): 764-771.
Silva J, Abebe W, Sousa SM, Duarte VG, Machado ML, and Matos FA. (2003). Analgesic and anti-inflammatory effects of essential oils of Eucalyptus. J. Ethnopharmacol. 89, 277–283.
Soares MCMS, Damiani CEN, Moreira CM, Stefanon I, Vassallo DV. 2005. Eucalyptol, an essential oil, reduces contractile activity in rat cardiac muscle. Brazilian Journal of Medical and Biological Research 38: 453-461.
Van Den Dool, H, Kratz PD. (1963). A generalization of the retention index system linear temperature programmed gas-liquid partition chromatography. Journal of Chromatography, 11, 463.
Akin M, Aktumsek A and Nostro A. (2010). Antibacterial activity and composition of the essential oils of Eucalyptus camaldulensis Dehn. and Myrtuscommunis L. growing in Northern Cyprus. African J. Biotech., 9 (4): 531-535
Bastos, VP, Brito TSTS, Lima FJB, Pinho JPM, Lahlou SS, Abreu Matos FJ, Santos AA and Magalhães PJ. (2009). Inhibitory effect of 1,8-cineole on guinea-pig airway challenged with ovalbumin involves a preferential action on electromechanical coupling crossref, 36(11):1120-6.
Bhatti HN, Iqbal Z, Chatha SAS AND Bukhari IH.(2007).Variations in Oil Potential and Chemical Composition of Eucalyptus crebra Among Different Districts of Punjab–Pakistan. Intern J Agricul& Biology, 9(1): 136- 138.
Braun L and Cohen M. (2007).Herba and natural supplements.2nd ed. Elsevier Australia.
Cheng SS, Huang CG, Chen YJ, Yu JJ, Chen WJ, Chang ST.( 2009). Chemical compositions and larvicidal activities of leaf essential oils from two eucalyptus species. J Bioresource Technology 100: 452–456.
Coelho-de-Souza LN, Leal-Cardoso JH, de Abreu Matos FJ, Lahlou S, Magalhães PJ. 2005. Relaxant effects of the essential oil of Eucalyptus tereticornis and its main constituent 1,8-cineole on guinea-pig tracheal smooth muscle. Planta Med. 71(12):1173-5.
Earley S, Gonzales AL, Garcia ZI. 2010. A dietary agonist of transient receptor potential cation channel V3 elicits endothelium-dependent vasodilation. MolPharmacol 77: 612-620.
Gruenwald J, Brendler andJaenicke . (2000). PDR for Herbal Medicines".Medical Economics Company, Inc. at Montvale.
Jucá DM, da Silva MT, Junior RC Jr, de Lima FJ, Okoba W, Lahlou S, de Oliveira RB, dos Santos AA and Magalhães PJ. (2011). The essential oil of Eucalyptus tereticornis and its constituents, α- and β-pinene, show accelerative properties on rat gastrointestinal transit. Planta Med. 77(1):57-9.
Kheder DA, and Al-Habib OAM.( 2013). Physiological Effects of Essential Oils of Eucalyptus camaldulensisDehn Fractions onIso;ated Aortaand Trachea in Male Albino Rats. PhD Thesis.
Lahlou, S, André FernandesFigueiredo, Pedro Jorge Caldas Magalhães, andJosé Henrique Leal-Cardoso. (2002). Cardiovascular effects of 1,8-cineole, a terpenoid oxide present in many plant essential oils, in normotensive rats. Can. J. Physiol. Pharmacol. 80: 1125–1131.
Lahlou S, Interaminense LF, Leal-Cardoso JH, Duarte GP (2003).Antihypertensive effects of the essential oil of Alpiniazerumbet and its main constituent, terpinen-4-ol, in DOCA-salt hypertensive conscious rats.FundamClinPharmacol 17: 323-330.
Lima FJ, Brito TS, Freire WB, Costa RC, Linhares MI, Sousa FC, Lahlou S, Leal-Cardoso JH, Santos AA, Magalhães PJ. (2010). The essential oil of Eucalyptus tereticornis, and its constituents alpha- and beta-pinene, potentiate acetylcholine-induced contractions in isolated rat trachea. Fitoterapia. 81(6):649-55.
Magalhães PJ, Lahlou S, Jucá DM, Coelho-De-Souza LN, Da Frota PT, Da Costa AM, Leal-Cardoso JH (2008). Vasorelaxation induced by the essential oil of Croton nepetaefolius and its constituents in rat aorta are partially mediated by the endothelium. FundamClinPharmacol 22: 169-177.
Magyar J, Szentandrássy N, Bányász T, Fülöp L, Varró A, Nánási PP( 2004). Effects of terpenoid phenol derivatives on calcium current in canine and human ventricular cardiomyocytes.Eur J Pharmacol 487: 29-36.
Nascimento NR, Refosco RM, Vasconcelos EC, Kerntopf MR, Santos CF, Batista FJ, De Sousa CM, Fonteles MC. (2009). 1,8-Cineole induces relaxation in rat and guinea-pig airway smooth muscle. J Pharm Pharmacol. 61(3):361-6.
Peixoto-Neves, D., Silva-Alves, K.S., Gomes, M.D.M., Lima, F.C., Lahlou, S., Magalhães, P.J.C., Ceccatto, V.M., Coelho-de-Souza, A.N. and Leal-Cardoso, J.H. (2010).Vasorelaxant effects of the monoterpenic phenol isomers, carvacrol and thymol, on rat isolated aorta. Fundamental & Clinical Pharmacology, 24: 341–350.
Perez-Hernandeza, N, Ponce-Montera H, Ortiza MI, Carino-Cortesa R and Joseph-Nathanb P. (2009).Structure-Activity Relationships of Aromadendranes in Uterus-Relaxant Activity. Z. Naturforsch. 64 c: 840 – 846.
Pinto, NV, Assreuy AM, Coelho-de-Souza AN, Ceccatto VM, Magalhães PJ, Lahlou S, Leal-Cardoso JH. (2009). Endothelium-dependent vasorelaxant effects of the essential oil from aerial parts of Alpiniazerumbet and its main constituent 1,8-cineole in rats.
Ribeiro TP, Porto DL, Menezes CP, Antunes AA, Silva DF, De Sousa DP, Nakao LS, Braga VA, Medeiros IA. (2010). Unravelling the cardiovascular effects induced by alpha-terpineol: a role for the nitric oxide-cGMP pathway. ClinExpPharmacol Physiol. 37(8):811-6.
Saito K, Okabe T, Inamori Y, Tsujibo H, Miyake Y, Hiraoka K, Ishida N (1996). The biological properties of monoterpenes: Hypotensive effects on rats and antifungal activities on plant pathogenic fungi of monoterpenes. MokuzaiGakkaishi 42: 677-680.
Santos, MRV, Moreira FV, Fraga BP, De Sousa DP, Bonjardim LR and Quintans-Junior LJ.( 2011). Cardiovascular effects of monoterpenes: a review. Brazilian J Pharmacognosy 21(4): 764-771.
Silva J, Abebe W, Sousa SM, Duarte VG, Machado ML, and Matos FA. (2003). Analgesic and anti-inflammatory effects of essential oils of Eucalyptus. J. Ethnopharmacol. 89, 277–283.
Soares MCMS, Damiani CEN, Moreira CM, Stefanon I, Vassallo DV. 2005. Eucalyptol, an essential oil, reduces contractile activity in rat cardiac muscle. Brazilian Journal of Medical and Biological Research 38: 453-461.
Van Den Dool, H, Kratz PD. (1963). A generalization of the retention index system linear temperature programmed gas-liquid partition chromatography. Journal of Chromatography, 11, 463.
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2013-06-30
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Al-Habib, O. A. M., Kheder, D. A., & Gilardoni, G. (2013). Relaxant Effects of Essential Oils Of Eucalyptus Camaldulensis on Aortic Rings in Male Albino Rats. Science Journal of University of Zakho, 1(1), 139–146. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/56
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