In Vitro Multiplication of Photinia (Photinia X Fraseri)Usingdifferent Culture Media

  • Layla Sh. Mohammed AL-Mizory Dohuk University
  • Ameena M. Hassan Duhok University
Keywords: Photinia x fraseri, tissue culture media, plant growth regulators

Abstract

A very high rate of multiple shoots was obtained from nodal explants of Photinia x fraseri on MS medium supplemented with a various concentration of Kin alone (1, 2, and 3) mgl-1alone and combination of Kin at (1, 2, and 3) mgl-1 with IBA or IAA at (0.2 and 0.4) mgl-1. The results show that the number of shoots/ explant was significantly affected the levels of Kin alone and combination with IBA or IAA. The number of shoots (6.00 shoot/ explant) when Kin added to the medium at 3mgl-1 in which it was significantly different from other Kin concentrations. While the effect of different medium with the fixed and best concentration of Kin+ IBA and Kin+ IAA on the average shoot number and leaves number /explant of Photinia explant (node segment and shoot tips) The highest number of shoots (5.90) was recorded in node segment when cultured on WPM containing Kin+ IBA and it was increased significantly compared with all treatments from shoot tip. However, the maximum number of leaves (20.10) was recorded on WPM medium supplemented with Kin+ IBA and it was increased significantly compared with all treatments which containing different media plus Kin at 2mgl-1 + 0.4mgl-1 IBA and Kin at 1mgl-1+ 0.4mgl-1 IAA.Shootlel form the combination of from photinia cultured on ¼MS medium improved 0.6 mgl-1NAA concentration produced the highest number of roots/shootlel (6.5roots/shootlet) compared with all treatments contains ½MS medium added IAA, IBA and NAA on all concentration. While, theshootlets from photinia cultured on ¼MS medium and supplemented with 0.6mgl-1NAA formed an average (5.29 cm) compared to all treatments containing ½MS medium. Such plantlets were successfully transferred to soil after hardening with a high rate of survival.

Author Biographies

Layla Sh. Mohammed AL-Mizory, Dohuk University

Horticulture Dept., Faculty of Agriculture and Forestry, Duhok University, Kurdistan Region, Iraq.

Ameena M. Hassan, Duhok University

Horticulture Dept., Faculty of Agriculture and Forestry, Duhok University, Kurdistan Region, Iraq.

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Published
2014-12-30
How to Cite
Mohammed AL-Mizory, L., & Hassan, A. (2014). In Vitro Multiplication of Photinia (Photinia X Fraseri)Usingdifferent Culture Media. Science Journal of University of Zakho, 2(2), 310-322. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/238
Section
Science Journal of University of Zakho