Aflp Assessment of Ficus Cultivars for Identification and Conservation

  • Shaymaa H. Ali University of Duhok
  • Jaladet M. S. Jubrael University of Duhok
Keywords: Fig (Ficus carica), AFLP, genetic similarity

Abstract

The fig (Ficus carica L., Moraceae), a typical Mediterranean fruit crop, is characterized by large adaptive potentialities to various ecological areas. To create a molecular characterization for 16 cultivars of Ficus, five  primer combinations were used to amplify these cultivar’s genomic DNA, producing a total of 292 legible bands (markers) were revealed, of which 281(96%) distinct polymorphic band patterns. The genetic diversity among 16 cultivars of fig ranged between 0.2124-0.7154. The lowest genetic distance was found between Qashe kani and Arzani where as the highest genetic distance was found between Qarani rash and Ribari. Cluster analysis by Using UPGMA method based on the similarity coefficient, cultivars were separated into seven major genetic clusters which were named as (F1, F2, F3, F4, F5, F6 and F7) with many sub-clusters. The analyzed data illustrates a good variability in the genetic pool of the local common fig making it a valuable source for incorporation into potential breeding programs for the region.

Author Biographies

Shaymaa H. Ali, University of Duhok

Department of Molecular Biology, Scientific Research Center, Faculty of Science, Duhok University, Kurdistan Region-Iraq

Jaladet M. S. Jubrael, University of Duhok

Department of Molecular Biology, Scientific Research Center, Faculty of Science, Duhok University, Kurdistan Region-Iraq

References

Aggawal P. K., Brar D. S., Nands S., Huang N. and Khush G. S. (1999). Phylogenetic relationship among Oryza species revealed by AFLP markers. Theor. Appl. Genet., 98:1320 – 1328.
Angliolillo A., Mencuccini M. & Baldoni L. (1999). Olive genetic diversity assessed using amplified fragment length polymorphisms. Theor Appl Genet 98: 411–421.
Bamikole M. A., Ikhatua U. J., Babayemi O. J., Arigbede O. M., Etela I., Osagie P. (2001). Assessment of forage acceptability, some nutritive and anti-nutritive components of Ficus species in Benin, Nigeria. In: Malau-Aduli, Adeyinka IA, Eds. Strategies for poverty alleviation: animal production option. Proceedings of the 26th Conference of the Nigerian Society for Animal Production (NSAP). Zaria, Nigeria; pp. 310-3.
Berg C. C. (1989). Classification and distribution of Ficus, Experientia 45 605–611.
Cabrita L. F., Aksoy U., Hepaksoy S., Leitao J. M. (2001). Suit ability of isozyme, RAPD and AFLP markers to assess genetic differences and relatedness among fig (Ficus carica L.) clones. Sci Hortic; 87: 261-73.
Cervera M. T., Cabezas J. A., Sancha J. C., Martinze de Toda F. and Martinz- Zapater J. M. (1998). Applications of AFLPs to the characterization of grapevine Vitis vinifera L. genetic resource. A case study with accessions from Rioja (Spain). Theor. Appl. Genet., 97: 51-59.
Chessa I., Niedu G., Serra P. (1998). Fig germplasm characterization using isozymes analysis, Acta Hortic. 480: 43–148.
De Masi L., Cipollaro M., Di Bernardo G. (2003). Clonal selection and molecular characterization by RAPD analysis of the fig (Ficus carica L.) “Dottato” and “Blanco del Cilento” cultivars in Italy. Acta Hortic; 605: 65-8.
Giraldo E., Viruel M. A., Lopez-Corrales M., Hormaza J. I. (2005). Characterisation and cross-species transferability of microsatellites in the common fig (Ficus carica L.). J Hortic Sci Biotechnol 80:217–224.
Han T. H., De Jeu M., Van Eck H. and Jacobsen E. (2000). Genetic diversity of Chilean and Brazilian Alstroemeria species assessed by AFLP analysis. Heredity, 84: 564-569.
Hedfi J., Trifi M., Hannachi-Salhi A., Ould M. S. A., Rhouma A., Marrakchi M. (2003). Morphological and izozymic polymorphisms in Tunisian fig (Ficus carica L.) collection. Acta Hortic; 605: 319-25.
Jaccard, P., 1908. Nouvelles recherches sur la distribution florale. Bull Soc Vaudoise Sci. Nat, 44: 223-270.
Jubrael J. M.S. (2005). Assessment of AFLP- based Genetic Relationships among Date Palm (Phoenix dactylifera L.) Varieties of Iraq. J. AMER. Soc. HoRT. Sci. 130(3):442-447.
Khadari B., Hochu I., Bouzid L., Santoni S., Roger J. P., Kjellberg F. (2003). The use of microsatellite markers for identification and genetic diversity evaluation of the fig collection in CBNMP. Acta Hortic; 605: 77-86.
Khadari B., Grout C., Santoni S., Kjellberg F. (2005). Contrasted genetic diversity and differentiation among Mediterranean populations of Ficus carica L.: a study using mtDNA RFLP. Genet Resour Crop Evol 52:97–109.
Le Febvre V. B., Goffinet B., Chauvet J. C., Caromel P., Signoret P., Brand R. and Palloix A. (2001). Evaluation of genetic distances between pepper inbred lines for cultivar protection purposes: comparison of AFLP, RAPD and phenotypic data. Theor. Appl. Genet., 102: 741-750.
Machado C. A., Jousselin E., Kjellberg F., Compton S. G., Herre E. A. (2001). Phylogenetic relationships, historical biogeography and character evolution of fig pollinating wasps, P. Roy. Soc. Lond. B Bio. 268: 7–10.
Matthes M., Singh R., Cheah S.C. and Karp A. (2001). Variation in oil palm (Elaeis guineensis Jacq.) tissue culturederived regenerants revealed by AFLPs with methylation-sensitive enzymes. Theor. Appl. Genet., 102: 971-979.
Maniatis T., Fritsch E. F. and Sambrook J. (2001). In vitro application of DNA by the Polymerase Chain Reaction, in Molecular Cloning: A Laboratory Manual. 2nd ed., Cold Spring Harbor Laboratory Press, New York, U.S.A., p. 691.
Manandhar N. P. (1998). Native phytotherapy among the Raute tribe of Dadeldhura district, Nepal. J Ethnopharmacol; 60: 199-206.
Monte-Corvo L., Cabrita L., Oliveira C., & Leitao J., (2000). Assessment of genetic relationships among Pyrus species and cultivars using AFLP and RAPD markers. Genet Resour Crop Ev 47: 257–265.
Nei M., and Li W. H. (1979). Mathematical moder for studding genetic variation in terms of restriction endonuclease. Pro: Nat. Acad. Sci. U.S.A. 74:5269-5273.
Rohlf F. J. (1993). NT SYS-PC. Numerical Taxonomy and Multivariate Analysis System. Version 1.8 Exter Software, Setauket, New York, U.S.A.
Russell J., Fuller J. D., Macaulay M., Hatz B. G., Jahoor A., Powell W. and Waugh R. (1997). Direct comparison of level of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theor. Appl. Genet., 95: 714-722.
Sadder M. T., Ateyyeh A. F. (2006). Molecular assessment of polymorphism among local Jordanian genotypes of common fig (Ficus carica L.). Sci Hortic Amsterdam 107:347–351.
Salhi-Hannachi A., Trifi M., Zehdi S., Hedhi J., Mars M., Rhouma A. (2004). Inter simple sequence repeat fingerprints to access genetic diversity in Tunisian fig (Ficus carica L.) germplasm. Genet Resour Crop Evol 51:269–275.
Satyavati G. V., Prasad D. N., Gupta B., Srivastava R. K. (1976). Studies on ulcerogenic activity of curcumin. Indian J Physiol Pharmacol; 20(2): 92-3.
Subramanian S. S., Nair A. G. R. (1970). Sterols and flavonols of Ficus bengalensis. Phytochemistry; 9(12): 2583-4.
Torres A. M. and Tisserat B. (1980). Leaf isozymes as genetic markers in date palms. Amer. J. Bot. 67:162-167.
Valdeyron G. (1976). Sur le système génétique du figuier (Ficus carica L.), Essai d’interprétation évolutive, Ann. Inst. Natl. Agron. 5.
Valdeyron G., Llyod D. G., (1979). Sex differences and flowering phenology in the common fig, Ficus carica L., Evolution 33 (2) 673– 685.
Vavilov N. I. (1951). The origin, variation, immunity and breeding of cultivated plants. The Ronald Press Company, New York.
Vos P. R., Hogers M., Bleeker M., Reijans T., van de Lee M., Hornes A., Fritjters J., Pot J., Peleman M., Kuiper & Zabeau M., (1995). AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23: 4407–4414.
Weigand F., Baum and Udupa S. (1993). DNA Molecular Marker Techniques. Technical Manual No.20. International Center for Agricultural Research in the Dry Areas (ICARDA). Aleppo, Syria.
Yuan L., Warburton M., Zhang S., Khairallah M., Liu X., Peng Z. and Li L. (2000). Comparison of genetic diversity among maize inbred lines based on RFLP, SSRs, AFLPs, and RAPDs. Yi Chuan Bao, 2: 723-733.
Zhu J., Gale M. D., Quarrie S., Jackson M. T. and Bryan G. J. (1998). AFLP markers for the study of rice biodiversity. Theor. Appl. Genet., 96: 602-611.
Zhukovsky P. M. (1962). Cultivated plants and their relatives (abridged translation by Hudson PS). Commonwealth Agricultural Bureaux, England.
Zohary D., Hopf M. (1988). Domestication of plants in the old world. Oxford: Clarendon Press.
Zohary D., Hopf M. (1993). Domestication of plants in the old world. Clarendon Press, Oxford.
Published
2013-09-30
How to Cite
Ali, S., & Jubrael, J. (2013). Aflp Assessment of Ficus Cultivars for Identification and Conservation. Science Journal of University of Zakho, 1(2), 642-650. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/322
Section
Science Journal of University of Zakho