Genetic Variation Assessment of Some Prunus Species Using Srap Markers

  • Sabrya J. Sabrya University of Duhok
  • Avesta M. Ali University of Duhok
  • Jaladet M. S. Jubrael University of Duhok
Keywords: SRAP marker, Prunus species, genetic diversity


Genetic variation at molecular level was evaluated among three species of Prunus genus and a wild species, (P. arabica (wild), P. argentea (wild), P. dulcis (local), and  P. dulcis (wild ). The genetic variation assessment was carried out using SRAP molecular markers. The genetic similarity coefficient revealed the genetic relationship among the samples tested in which the highest genetic distance was between P. dulcis (local), and  P. dulcis (wild), and lowest genetic distance was between P. arabica (wild), P. argentea  (wild). The phylogenetic tree was obtained using UPGMA method depending on the total number of SRAP bands. There were two main groups in the dendrogram: the first one consists of two subgroup: P. arabica and P. argentea cluster together in one Subgroup and P. dulcis (wild) appear alone in this subgroup. P. dulcis (Local variety) appear in the second group alone.

Author Biographies

Sabrya J. Sabrya, University of Duhok

Scientific Research Center, University of Duhok, Kurdistan Reqion – Iraq.

Avesta M. Ali, University of Duhok

Scientific Research Center, University of Duhok, Kurdistan Reqion – Iraq.

Jaladet M. S. Jubrael, University of Duhok

Scientific Research Center, University of Duhok, Kurdistan Reqion – Iraq.


Ahmed, I., M. Islam, A. Mannan, R. Naeem and B. Mirza. 2009. Optimization of conditions for assessment of genetic diversity in barley (Hordeum vulgare L.) using microsatellite markers. Barley Genet. Newslett., 39: 5-12.
Aradhya M. K., Weeks C., Simon C.W. (2004). Molecular characterization of variability and relationships among seven cultivated and selected wild species of Prunus L. using Amplified Fragment Length Polymorphism. Sci. ort. 103:131- 114.
Cohen J.I., Williams JT, Plucknett DL, Shands HL (1991) Ex situ conservation of plant genetic resources: global development and environmental concerns. Science 253:866–872.
Evans, L.T. 1997. Adapting and improving crops: the endless task. Philosophical Transactions of the Royal Society of London: Biological Sciences 352:901-906.
Fu, J., M.F. Zhang and X.H. Qi. 2006. Genetic diversity of traditional Chinese mustard crops Brassica juncea as revealed by phenotypic differences and RAPD markers. Genet. Resou. & Crop Evol., 53: 1513-1519.
Graham, J., K. Smith, K. MacKenzie, L. Jorgenson, C. Hackett and W. Powell. 2004. The construction of a genetic linkage map of red raspberry based on AFLP’s genomic SSR markers. Theor. Appl. Genet., 109: 740-749.
Laidò, G., Mangini, G., Taranto, F., Gadaleta, A., Blanco, A., Cattivelli, L., Marone D., Mastrangelo, A, M., PapaR., and De Vita, P. (2013). Genetic diversity and population structure of tetraploid wheats ( Triticum turgidum L.) estimated by SSR, DArT and pedigree data. PLOS ONE 8: e 67280.
Lewer, K.S., C.A. Saski, B.J. Cuthbertson, D.C. Henry, M.E. Staton, D.S. Main, A.L. Dhanaraj, L.J.
Rowland and J.P. Tomkins. 2008. A blackberry expressed sequence tag library for the development of simple sequence repeat markers. BMC Plant Biol., 8: 69-76.
Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals Genetics 89:583-590.
Rao V.R. and Hodgkin T. (2002). Genetic diversity and conservation and utilization of plant genetic Resources. Plant Cell, Tissue and Organ Culture 68: 1–19, 2002
Sargent, D.J., F. Fernandez, A. Rys, V.H. Knight, D.W. Simpson and R.K. Tobutt. 2007. Mapping of A1 conferring resistance to the aphid Amphorophora idaei dw (dwarfing habit) in red raspberry using AFLP and micro-satellite markers. BMC Plant Biol., 7: 15.
Sreekanth, P. M., Balasundaran, M., Nazeem, P. A., and Suma, T. B. 2012. Genetic diversity of nine natural Tectona grandis L.f. locations of the Western Ghats in Southernern India. Conserv. Genet. 13: 1409-1419.
Weigand, F., Baum M. and Udupa S. (1993). DNA molecular marker technics, technical manual, No. 20 International Center for Agricultural Research in the Dry Areas (ICARDA). Aleppo, Syria.
How to Cite
Sabrya, S., Ali, A., & Jubrael, J. (2016). Genetic Variation Assessment of Some Prunus Species Using Srap Markers. Science Journal of University of Zakho, 4(2), 173-176. Retrieved from
Science Journal of University of Zakho