Molecular Characterization of Echinococcus Granulosus Isolated from Human Hydatid Cyst Using Mitochondrial Cox1 Gene Sequencing in Dohuk Province- Kurdistan Region, Iraq

  • BAYRAM D. AHMED Central Public Health laboratory
  • WIJDAN M.S. MERO University of Zakho
  • A.M. SALIH University of Duhok
  • NING, XIAO Sichwan Center for Parasitic Disease Control, China.
  • ADRIANO CASULLI Dept. of Infectious, Parasitic and Immunomediated Diseases, Rome, Italy.
  • JASIM M. ABDO University of Duhok
Keywords: E. granulosus, Strain identification, G1 Sheep strain, Microvariants Genotype

Abstract

 

To characterize the circulating E. granulosus genotype in Duhok province, a total of 17 human (14 female and 3 male) hydatid cysts (10 from lung and 7 from liver) were subjected to molecular analysis using mitochondrial cox1 gene sequencing. Total DNA was extracted using Qiagene DNeasy blood and tissue mini kit then it was electrophoresed on 1.5% Agarose gel in TBE 0.5% buffer for 20 minutes and sequenced. Variable sequences of 418bp were obtained for 15 out of 17 samples. The sequences were aligned with old sequences of G1, G2, G3 genotypes and the new Turkish and Iranian genotypes. As a result, the Duhok isolates were more identical to the newer Turkish and Iranian G1 common sheep genotype sequences than the old ones due to the occurrence of different multiple nucleotide substitutions among nearly half of Duhok isolates. In conclusion, the results of this study showed the presence of the G1-G3 cluster sensu stricto cluster genotype in Duhok province, therefore preventive measures should be arranged accordingly.

Author Biographies

BAYRAM D. AHMED, Central Public Health laboratory

Central Public Health laboratory, Kurdistan Region-Iraq.

WIJDAN M.S. MERO, University of Zakho

University of Zakho, Faculty of Science, Kurdistan Region-Iraq.

A.M. SALIH, University of Duhok

Faculty of Science, University of Duhok, Kurdistan Region-Iraq.

NING, XIAO, Sichwan Center for Parasitic Disease Control, China.

Sichwan Center for Parasitic Disease Control, China.

ADRIANO CASULLI, Dept. of Infectious, Parasitic and Immunomediated Diseases, Rome, Italy.

Dept. of Infectious, Parasitic and Immunomediated Diseases, Rome, Italy.

JASIM M. ABDO, University of Duhok

Faculty of Veterinary Medicine, University of Duhok, Kurdistan Region-Iraq.

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Published
2013-06-30
How to Cite
AHMED, B., MERO, W., SALIH, A., XIAO, N., CASULLI, A., & ABDO, J. (2013). Molecular Characterization of Echinococcus Granulosus Isolated from Human Hydatid Cyst Using Mitochondrial Cox1 Gene Sequencing in Dohuk Province- Kurdistan Region, Iraq. Science Journal of University of Zakho, 1(1), 72-80. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/40
Section
Science Journal of University of Zakho

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