In Vitro Rooting Of Hybrid Rose (Rosa Carolina L.)Shoots and Subsequent Establishment
Keywords:
In vitro, Hybrid rose, Rosa carolina, RootingAbstract
A successful in vitro propagation system has been developed for the valuable ornamental plant hybrid rose (Rosa carolina L.) by in vitro culture of shoot tips from mature plants. First shoot tip explants were excised and cultured on basal medium of Murashige and Skoog (1962) containing several concentrations of BAP and Kinetin. Multiple shoot formation of up to 3 shoots was obtained on MS medium supplemented with 4.0 mg/l BAP. Induced microshoots was then tested for root initiation on full MS medium supplemented with different concentrations of Indolbutyric acid (IBA) and Naphthalene acetic acid (NAA) and the best root formation was observed on a medium containing 1.0 and 2.0 mg/l of IBA and 2.0 mg/l of NAA. The regenerated plants were successfully transplanted to the greenhouse and appeared similar characteristics to the parent plants in morphologic features.
References
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Soomro, R.; Yasmin,S. and Aleem,R. (2003). In vitro propagation of Rosa indica. Pakistan J.of Biological Sciences, 6(9): 826-830.
Senapati, S.K. and Rout,G.R. (2008). Study of culture conditions for improved micropropagation of hybrid rose. Hort.Sci., 35(1): 27-35.
Yan,M.; Byrne, D.H.and Jing,C. (1996). Propagation of rose species in vitro. In vitro Cellular and Developmental Biology Plant, 32: 103-108.
Xing,W.; Bao,M.; Qin,H. and Ning,G. (2010). Micropropagation of Rosa rugosa through axillary shoot proliferation.Acta Biologica Cracoviensia Series Botanica, 52(2): 69-75.
Bressan, P.H.; Kim,Y.J.; Hyndman,S.E.; Hasegawa,P.M. and Bressan,R.A. (1982).Factors affecting in vitro propagation of rose. Journal of American Society Horticulture Science 107: 979-90.
Barve, D.M.; Lyenr, R.S. ; Kendurkar, S. and Mascarenhas, A.F. (1984). An efficient method for rapid propagation of some budded rose varieties. Ind. J. of Hort., 41:1-7.
Carelli, B.P. and Echeverrigaray, S. (2002). An improved system for the in vitro propagation of rose cultivars. Scientia Horticulturae 92:69-74.
Davies, D.R. (1980). Rapid propagation of roses in vitro. Scientia Horticulturae, 13: 385-9.
Dhawan, V. and Bhojwani, S.S. (1986). Micropropagation in crop plants. Glimpses Plant Res., 7:1-75.
Douglas, G.C.; Rutledge, C.B.; Casey, A.D. and Richardson, D.H.S. (1989). Micropropagation of floribunda ground cover and miniature roses. Plant Cell Tissue Organ Culture, 19: 55-64.
Economon , A .S . and Spanodaki, M. J. (1985). In vitro propagation of Gardenia. HortScience, 20: 213.
Horn, W.A.H. (1992). Micropropagation of rose (Rosa L.).In: Bajaj Y.P.S. (Eds). Biotechnology in agriculture and forestry. High-tech and micropropagation IV. Springer. Germany, Vol:20.
Kanchanapoom, K.; Sakpeth, P.and Kanchanapoom,K.(2010). In vitro flowering of shoots regenerated from cultured nodal explants of Rosa hybrida cv. ‘Heirloom’.Science Asia 36:161-164.
Maheswaran, M.; Gnanam, R. and Sreerangasamy, S. R. (2000). Plant Tissue Culture, Principlesand Practices. CPMB.
Murashige, T. and F. Skoog. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. , 15: 473-497.
Murashige, T.; M. Serpa. and J. B. Jones (1974). Clonal multiplication of gerbera through tissue culture. Hortscience, 9: 175-180.
Martin, C. (1985). Plant breeding in vitro. Endeavour, 9:81-6.
Nak-Udom, N.; Kanchanapoom,K. and Kanchanapoom,K. (2009). Micropropagation from cultured nodal explants of rose (Rosa hybrid L.cv. Perfume Delight,). Songklanakarin J. Sci. Technol., 31:583-586.
Pierik, R.L.M. (1991). Horticulture new technologies and application Proceeding of the international seminar on new frontiers in horticulture. Curr.Plant Sci. Biolechnol.Agric.,12:141-153.
Pratheesh, P. T. and Anilkumar,M. (2012).In vitro flowering in Rosa in indica L. Inter. J. of Pharmacy and Biological Sciences, 2:196-200.
Razavizadeh, R. and Eshanpour, A.A.(2008). Optimization of in vitro propagation of Rosa hybrid L. Cultivar Black Red. American-Eurasian J.Agric & Environ.Sci., 3(1): 96-99.
Salman, M. A. (1988). Fundamentals of plant Cell and Tissue Culture. Ministry of Higher Education and Scientific Research. P.375 (in Arabic).
Salekjalai, M.; Jafari, B. and Tarinejad,A. (2011). Iv vitro multiplication of Rose (Rosa hybriad.cv.Baccara). American-Eurasian J. Agric & Environ.Sci., 11(1): 111-116.
SAS. (2001). SAS/STAT Vser's Guide for personal Computers. Release 6.12 SAS Institute Inc. Cary, NC, USA.
Soomro, R.; Yasmin,S. and Aleem,R. (2003). In vitro propagation of Rosa indica. Pakistan J.of Biological Sciences, 6(9): 826-830.
Senapati, S.K. and Rout,G.R. (2008). Study of culture conditions for improved micropropagation of hybrid rose. Hort.Sci., 35(1): 27-35.
Yan,M.; Byrne, D.H.and Jing,C. (1996). Propagation of rose species in vitro. In vitro Cellular and Developmental Biology Plant, 32: 103-108.
Xing,W.; Bao,M.; Qin,H. and Ning,G. (2010). Micropropagation of Rosa rugosa through axillary shoot proliferation.Acta Biologica Cracoviensia Series Botanica, 52(2): 69-75.
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Published
2013-06-30
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DANIAL, G. H., & OMER, M. S. (2013). In Vitro Rooting Of Hybrid Rose (Rosa Carolina L.)Shoots and Subsequent Establishment. Science Journal of University of Zakho, 1(1), 147–152. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/57
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