The Integrity of DNA Extracted from Oryctolagus Cuniculus Carcasses Treated With Different Corrosive Chemical Substances

  • Onyekachi O. Iroanya Dept. of Cell Biology and Genetics University of Lagos https://orcid.org/0000-0002-5122-1466
  • Jeffrey O. Ogbeide Dept. of Cell Biology and Genetics, University of Lagos
  • Tochukwu F. Egwuatu Dept. of Cell Biology and Genetics, University of Lagos
Keywords: DNA integrity, DNA degradation, Bone, Tissue, corrosive chemicals, hydrochloric acid, Sodium hydroxide

Abstract

There is a gap in the literature concerning the use of corrosive chemicals to erode human body. The aim of this study is to determine the integrity of DNA samples extracted from rabbits’ carcasses immersed in different chemical. Twenty-one healthy rabbits were grouped into seven. Animals in group 1 were enclosed in transparent containers while groups 2 to 7 were submerged in 1% hydrochloric acid (HCl), sodium hypochlorite (NaClO), calcium hypochlorite (CaOCl2), 7.3% sodium hydroxide (NaOH), water and 35% HCl respectively. Tissue sample were collected on day(s) 1, 3, 7 and 12 for DNA extraction and analysis. The most destructive agent in this study was 35% HCl where the bones and almost all tissues degraded within 24hrs, followed by Sodium hydroxide while, there was no marked degradation in NaClO, CaOCl2, water and dilute HCl groups. Distinct, observable effects of each chemical on the extracted DNA samples revealed decreased DNA integrity and degradation at various time frames. Further studies need to be carried out to ascertain the concentration of these chemicals especially NaOH that will completely destroy the body of the rabbits within a short period of time. Chromosomal aberration analysis should also be included in future research.

Author Biographies

Onyekachi O. Iroanya, Dept. of Cell Biology and Genetics University of Lagos

Dept. of Cell Biology and Genetics, University of Lagos, Nigeria (oiroany@unilag.edu.ng)

Jeffrey O. Ogbeide, Dept. of Cell Biology and Genetics, University of Lagos

Dept. of Cell Biology and Genetics, University of Lagos, Nigeria (jeffzyme@gmail.com)

Tochukwu F. Egwuatu, Dept. of Cell Biology and Genetics, University of Lagos

Dept. of Cell Biology and Genetics, University of Lagos, Nigeria (tochukwufrankegwuatu@gmail.com)

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Published
2020-06-30
How to Cite
Iroanya, O., Ogbeide, J., & Egwuatu, T. (2020). The Integrity of DNA Extracted from Oryctolagus Cuniculus Carcasses Treated With Different Corrosive Chemical Substances. Science Journal of University of Zakho, 8(2), 42-47. https://doi.org/10.25271/sjuoz.2020.8.2.714
Section
Science Journal of University of Zakho