Human Scalp Hair Follicles Express Prostaglandin E2 Lipid Mediator and Receptors For Pge2
Keywords:
Alopecia, Hair Follicle, Immunohistochemistry, Lipidomics, Prostaglandin E2 receptor, RT-PCRAbstract
The hair follicle, which is a unique characteristic of the mammals, plays a vital role in a personal appearance and self-image confidence throughout the life and hair growth disorders cause serious psychological distress. The common pathological abnormalities are abnormal hair loss (alopecia) and excessive hair growth (hirsutism), but they are poorly controlled. Therefore an improved treatment for these disorders requires a better understanding of the mechanisms regulating hair growth. Prostaglandin analogues such as latanoprost, which are used for glaucoma treatment, also makes eyelashes grow longer. The mechanism of action by which these drugs stimulate eyelash hair growth is poorly understood. To determine whether PGE2 lipid mediator and receptors for PGE2 (EP2) are expressed in scalp hair follicle, electrospray tandem mass spectrometry coupled to liquid chromatography (LC/ESI-MS/MS), RT-PCR and Immunohistochemistry were carried out. Human scalp skin from non-balding areas (occipital and parietal) was obtained from healthy donors undergoing elective cosmetic surgery; with appropriate ethical approval. Isolated scalp hair follicle expresses PGE2 lipid mediator genes and protein for EP2. So isolated human scalp follicles contain PGE2 lipid mediator and express genes and protein for EP2 indicating that PGE2 may play a vital role in regulating hair growth, which may lead to a new method for hair loss treatment.
References
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Colombe, L., A. Vindrios, et al. (2007). "Prostaglandin metabolism in human hair follicle." Exp Dermatol 16(9): 762-769.
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Gulec, A. T., N. Tanriverdi, et al. (2004). "The role of psychological factors in alopecia areata and the impact of the disease on the quality of life." Int J Dermatol 43(5): 352-356.
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Jahoda, C. A. (1992). "Induction of follicle formation and hair growth by vibrissa dermal papillae implanted into rat ear wounds: vibrissa-type fibres are specified." Development 115(4): 1103-1109.
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Jansen, V. A. and M. van Baalen (2006). "Altruism through beard chromodynamics." Nature 440(7084): 663-666.
Kaufman, K. D., E. A. Olsen, et al. (1998). "Finasteride in the treatment of men with androgenetic alopecia. Finasteride Male Pattern Hair Loss Study Group." J Am Acad Dermatol 39(4 Pt 1): 578-589.
Kempen, E. C., P. Yang, et al. (2001). "Simultaneous quantification of arachidonic acid metabolites in cultured tumor cells using high-performance liquid chromatography/electrospray ionization tandem mass spectrometry." Anal Biochem 297(2): 183-190.
Masoodi, M. and A. Nicolaou (2006). "Lipidomic analysis of twenty-seven prostanoids and isoprostanes by liquid chromatography/electrospray tandem Colombe, L., J. F. Michelet, et al. (2008). "Prostanoid receptors in anagen human hair follicles." Exp Dermatol 17(1): 63-72.
Colombe, L., A. Vindrios, et al. (2007). "Prostaglandin metabolism in human hair follicle." Exp Dermatol 16(9): 762-769.
Curran, M. P. (2009). "Bimatoprost: a review of its use in open-angle glaucoma and ocular hypertension." Drugs Aging 26(12): 1049-1071.
Durn, J. H., K. M. Marshall, et al. (2010). "Lipidomic analysis reveals prostanoid profiles in human term pregnant myometrium." Prostaglandins Leukot Essent Fatty Acids 82(1): 21-26.
Garg, S. and A. G. Messenger (2009). "Alopecia areata: evidence-based treatments." Semin Cutan Med Surg 28(1): 15-18.
Girman, C. J., T. Rhodes, et al. (1998). "Effects of self-perceived hair loss in a community sample of men." Dermatology 197(3): 223-229.
Gulec, A. T., N. Tanriverdi, et al. (2004). "The role of psychological factors in alopecia areata and the impact of the disease on the quality of life." Int J Dermatol 43(5): 352-356.
Hamilton, J. B. (1951). "Patterned loss of hair in man; types and incidence." Ann N Y Acad Sci 53(3): 708-728.
Jahoda, C. A. (1992). "Induction of follicle formation and hair growth by vibrissa dermal papillae implanted into rat ear wounds: vibrissa-type fibres are specified." Development 115(4): 1103-1109.
Jahoda, C. A., K. A. Horne, et al. (1984). "Induction of hair growth by implantation of cultured dermal papilla cells." Nature 311(5986): 560-562.
Jansen, V. A. and M. van Baalen (2006). "Altruism through beard chromodynamics." Nature 440(7084): 663-666.
Kaufman, K. D., E. A. Olsen, et al. (1998). "Finasteride in the treatment of men with androgenetic alopecia. Finasteride Male Pattern Hair Loss Study Group." J Am Acad Dermatol 39(4 Pt 1): 578-589.
Kempen, E. C., P. Yang, et al. (2001). "Simultaneous quantification of arachidonic acid metabolites in cultured tumor cells using high-performance liquid chromatography/electrospray ionization tandem mass spectrometry." Anal Biochem 297(2): 183-190.
Masoodi, M. and A. Nicolaou (2006). "Lipidomic analysis of twenty-seven prostanoids and isoprostanes by liquid chromatography/electrospray tandem mass spectrometry." Rapid Commun Mass Spectrom 20(20): 3023-3029.
Messenger, A. G. and J. Rundegren (2004). "Minoxidil: mechanisms of action on hair growth." Br J Dermatol 150(2): 186-194.
Michelet, J. F., S. Commo, et al. (1997). "Activation of cytoprotective prostaglandin synthase-1 by minoxidil as a possible explanation for its hair growth-stimulating effect." J Invest Dermatol 108(2): 205-209.
Nishimura, E. K., S. R. Granter, et al. (2005). "Mechanisms of hair graying: incomplete melanocyte stem cell maintenance in the niche." Science 307(5710): 720-724.
Noble, J. E. and M. J. Bailey (2009). "Quantitation of protein." Methods Enzymol 463: 73-95.
Randall, V. A. (2001). "Is alopecia areata an autoimmune disease?" Lancet 358(9297): 1922-1924.
Randall, V. A. (2007). "Hormonal regulation of hair follicles exhibits a biological paradox." Semin Cell Dev Biol 18(2): 274-285.
Reynolds, A. J., C. Lawrence, et al. (1999). "Trans-gender induction of hair follicles." Nature 402(6757): 33-34.
Rogers, N. E. and M. R. Avram (2008). "Medical treatments for male and female pattern hair loss." J Am Acad Dermatol 59(4): 547-566; quiz 567-548.
Samad, T. A., A. Sapirstein, et al. (2002). "Prostanoids and pain: unraveling mechanisms and revealing therapeutic targets." Trends Mol Med 8(8): 390-396.
Shorter, K., N. P. Farjo, et al. (2008). "Human hair follicles contain two forms of ATP-sensitive potassium channels, only one of which is sensitive to minoxidil." FASEB J 22(6): 1725-1736.
Sotiropoulou, P. A., A. Candi, et al. "Bcl-2 and accelerated DNA repair mediates resistance of hair follicle bulge stem cells to DNA-damage-induced cell death." Nat Cell Biol 12(6): 572-582.
Taylor, A. W., R. S. Bruno, et al. (2006). "Benefits of prolonged gradient separation for high-performance liquid chromatography-tandem mass spectrometry quantitation of plasma total 15-series F-isoprostanes." Anal Biochem 350(1): 41-51.
Whiting, D. A., E. A. Olsen, et al. (2003). "Efficacy and tolerability of finasteride 1 mg in men aged 41 to 60 years with male pattern hair loss." Eur J Dermatol 13(2): 150-160.
Wise, H., Y. H. Wong, et al. (2002). "Prostanoid signal integration and cross talk." Neurosignals 11(1): 20-28.
Yang, P., E. Felix, et al. (2002). "Quantitative high-performance liquid chromatography/electrospray ionization tandem mass spectrometric analysis of 2- and 3-series prostaglandins in cultured tumor cells." Anal Biochem 308(1): 168-177.
Ziboh, V. A., C. C. Miller, et al. (2000). "Significance of lipoxygenase-derived monohydroxy fatty acids in cutaneous biology." Prostaglandins Other Lipid Mediat 63(1-2): 3-13.
Colombe, L., A. Vindrios, et al. (2007). "Prostaglandin metabolism in human hair follicle." Exp Dermatol 16(9): 762-769.
Curran, M. P. (2009). "Bimatoprost: a review of its use in open-angle glaucoma and ocular hypertension." Drugs Aging 26(12): 1049-1071.
Durn, J. H., K. M. Marshall, et al. (2010). "Lipidomic analysis reveals prostanoid profiles in human term pregnant myometrium." Prostaglandins Leukot Essent Fatty Acids 82(1): 21-26.
Garg, S. and A. G. Messenger (2009). "Alopecia areata: evidence-based treatments." Semin Cutan Med Surg 28(1): 15-18.
Girman, C. J., T. Rhodes, et al. (1998). "Effects of self-perceived hair loss in a community sample of men." Dermatology 197(3): 223-229.
Gulec, A. T., N. Tanriverdi, et al. (2004). "The role of psychological factors in alopecia areata and the impact of the disease on the quality of life." Int J Dermatol 43(5): 352-356.
Hamilton, J. B. (1951). "Patterned loss of hair in man; types and incidence." Ann N Y Acad Sci 53(3): 708-728.
Jahoda, C. A. (1992). "Induction of follicle formation and hair growth by vibrissa dermal papillae implanted into rat ear wounds: vibrissa-type fibres are specified." Development 115(4): 1103-1109.
Jahoda, C. A., K. A. Horne, et al. (1984). "Induction of hair growth by implantation of cultured dermal papilla cells." Nature 311(5986): 560-562.
Jansen, V. A. and M. van Baalen (2006). "Altruism through beard chromodynamics." Nature 440(7084): 663-666.
Kaufman, K. D., E. A. Olsen, et al. (1998). "Finasteride in the treatment of men with androgenetic alopecia. Finasteride Male Pattern Hair Loss Study Group." J Am Acad Dermatol 39(4 Pt 1): 578-589.
Kempen, E. C., P. Yang, et al. (2001). "Simultaneous quantification of arachidonic acid metabolites in cultured tumor cells using high-performance liquid chromatography/electrospray ionization tandem mass spectrometry." Anal Biochem 297(2): 183-190.
Masoodi, M. and A. Nicolaou (2006). "Lipidomic analysis of twenty-seven prostanoids and isoprostanes by liquid chromatography/electrospray tandem Colombe, L., J. F. Michelet, et al. (2008). "Prostanoid receptors in anagen human hair follicles." Exp Dermatol 17(1): 63-72.
Colombe, L., A. Vindrios, et al. (2007). "Prostaglandin metabolism in human hair follicle." Exp Dermatol 16(9): 762-769.
Curran, M. P. (2009). "Bimatoprost: a review of its use in open-angle glaucoma and ocular hypertension." Drugs Aging 26(12): 1049-1071.
Durn, J. H., K. M. Marshall, et al. (2010). "Lipidomic analysis reveals prostanoid profiles in human term pregnant myometrium." Prostaglandins Leukot Essent Fatty Acids 82(1): 21-26.
Garg, S. and A. G. Messenger (2009). "Alopecia areata: evidence-based treatments." Semin Cutan Med Surg 28(1): 15-18.
Girman, C. J., T. Rhodes, et al. (1998). "Effects of self-perceived hair loss in a community sample of men." Dermatology 197(3): 223-229.
Gulec, A. T., N. Tanriverdi, et al. (2004). "The role of psychological factors in alopecia areata and the impact of the disease on the quality of life." Int J Dermatol 43(5): 352-356.
Hamilton, J. B. (1951). "Patterned loss of hair in man; types and incidence." Ann N Y Acad Sci 53(3): 708-728.
Jahoda, C. A. (1992). "Induction of follicle formation and hair growth by vibrissa dermal papillae implanted into rat ear wounds: vibrissa-type fibres are specified." Development 115(4): 1103-1109.
Jahoda, C. A., K. A. Horne, et al. (1984). "Induction of hair growth by implantation of cultured dermal papilla cells." Nature 311(5986): 560-562.
Jansen, V. A. and M. van Baalen (2006). "Altruism through beard chromodynamics." Nature 440(7084): 663-666.
Kaufman, K. D., E. A. Olsen, et al. (1998). "Finasteride in the treatment of men with androgenetic alopecia. Finasteride Male Pattern Hair Loss Study Group." J Am Acad Dermatol 39(4 Pt 1): 578-589.
Kempen, E. C., P. Yang, et al. (2001). "Simultaneous quantification of arachidonic acid metabolites in cultured tumor cells using high-performance liquid chromatography/electrospray ionization tandem mass spectrometry." Anal Biochem 297(2): 183-190.
Masoodi, M. and A. Nicolaou (2006). "Lipidomic analysis of twenty-seven prostanoids and isoprostanes by liquid chromatography/electrospray tandem mass spectrometry." Rapid Commun Mass Spectrom 20(20): 3023-3029.
Messenger, A. G. and J. Rundegren (2004). "Minoxidil: mechanisms of action on hair growth." Br J Dermatol 150(2): 186-194.
Michelet, J. F., S. Commo, et al. (1997). "Activation of cytoprotective prostaglandin synthase-1 by minoxidil as a possible explanation for its hair growth-stimulating effect." J Invest Dermatol 108(2): 205-209.
Nishimura, E. K., S. R. Granter, et al. (2005). "Mechanisms of hair graying: incomplete melanocyte stem cell maintenance in the niche." Science 307(5710): 720-724.
Noble, J. E. and M. J. Bailey (2009). "Quantitation of protein." Methods Enzymol 463: 73-95.
Randall, V. A. (2001). "Is alopecia areata an autoimmune disease?" Lancet 358(9297): 1922-1924.
Randall, V. A. (2007). "Hormonal regulation of hair follicles exhibits a biological paradox." Semin Cell Dev Biol 18(2): 274-285.
Reynolds, A. J., C. Lawrence, et al. (1999). "Trans-gender induction of hair follicles." Nature 402(6757): 33-34.
Rogers, N. E. and M. R. Avram (2008). "Medical treatments for male and female pattern hair loss." J Am Acad Dermatol 59(4): 547-566; quiz 567-548.
Samad, T. A., A. Sapirstein, et al. (2002). "Prostanoids and pain: unraveling mechanisms and revealing therapeutic targets." Trends Mol Med 8(8): 390-396.
Shorter, K., N. P. Farjo, et al. (2008). "Human hair follicles contain two forms of ATP-sensitive potassium channels, only one of which is sensitive to minoxidil." FASEB J 22(6): 1725-1736.
Sotiropoulou, P. A., A. Candi, et al. "Bcl-2 and accelerated DNA repair mediates resistance of hair follicle bulge stem cells to DNA-damage-induced cell death." Nat Cell Biol 12(6): 572-582.
Taylor, A. W., R. S. Bruno, et al. (2006). "Benefits of prolonged gradient separation for high-performance liquid chromatography-tandem mass spectrometry quantitation of plasma total 15-series F-isoprostanes." Anal Biochem 350(1): 41-51.
Whiting, D. A., E. A. Olsen, et al. (2003). "Efficacy and tolerability of finasteride 1 mg in men aged 41 to 60 years with male pattern hair loss." Eur J Dermatol 13(2): 150-160.
Wise, H., Y. H. Wong, et al. (2002). "Prostanoid signal integration and cross talk." Neurosignals 11(1): 20-28.
Yang, P., E. Felix, et al. (2002). "Quantitative high-performance liquid chromatography/electrospray ionization tandem mass spectrometric analysis of 2- and 3-series prostaglandins in cultured tumor cells." Anal Biochem 308(1): 168-177.
Ziboh, V. A., C. C. Miller, et al. (2000). "Significance of lipoxygenase-derived monohydroxy fatty acids in cutaneous biology." Prostaglandins Other Lipid Mediat 63(1-2): 3-13.
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2013-09-30
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Khidhir, K. G. (2013). Human Scalp Hair Follicles Express Prostaglandin E2 Lipid Mediator and Receptors For Pge2. Science Journal of University of Zakho, 1(2), 533–542. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/263
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