Detection of Cpegene in Stool Samples of Food Poisoning Patients in Mosul/Iraq
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Clostridium perfringens, Food poisoning, Nested PCR, Cpe gene
Abstract
In this study, 27 stool samples collected from food poisoning patients in Mosul city were analyzed by both bacteriological standard methods and molecular methods. The bacteriological methods used for enumeration of C. perfringens while the nested PCR technique was used for detection cpe gene directly in stool samples in a method consists of a combination of nested PCR reaction with enrichment of the sample. Two pairs of oligonucleotide primers were used: the first primer pair amplifies a 425-bp fragment and the second pair amplifiesa199-bp within the 425-bp. Results showed that the number of C. perfringens were less than 103cell/g gave a 199-bp amplified fragment which indicated that those samples contain cpe gene. When we compared nested PCR result with the number of C. perfringens,7 out of 9 samples(77. 8%) which have greater than 103of C. perfringens per g were positive for cpe gene and 8 out of 18 samples(44. 4%) which have less than 103 of C. perfringens per g also gave cpe gene positive result.
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Copyright (c) 2013 ALAA H. AL-MOLA, AMERA M. AL-RAWI
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