Multilocus Sequence Typing of Klebsiella Pneumoniae Producing Extended Spectrum Β-Lactamases Isolated From Kurdistan Region, Iraq.
Keywords:
Klebsiella pneumoniae, ESBL, MLSTAbstract
This study was purposed to sequence analysis of ESBLs genotype of K pneumoniae using partial sequence and Multilocus sequence typing (MLST). A total of 275 K. pneumoniae isolatesinvolved three general hospitals in Duhok, Erbil, and Sulymania, from September 2010 to June 2011. The Minimum Inhibitory Concentration (MIC) was measured by Phoenix system that confirmed 187 ESBL producing isolates followed by the Double Disk Synergy Test (DDST). Then, 12 isolates were selectedaccording to sample diversity, high resistancy to β-lactam and cephalosporins and harboring combination of three genes (TEM, SHV, and CTX-M). Partial sequence analysis of TEM; showed two different genotypes regarding blaTEM as 9 isolates (75%) from different samples (wound, sputum and blood) from three provinces harbor TEM-1 gene and 3 isolates (25%) only from urine in three provinces harbor TEM-198 gene. SHV analysis revealed characterization of selected isolates into six different genotypes. The common genotype was blaSHV-11 involved five isolates from sputum and blood in Erbil and Sulymania provinces, and wound in Duhok province. Only one genotype as all 12 isolates (100%) from different samples and different provinces was found harbored CTX-M-15 gene. Multilocus sequence typing (MLST) study performed using seven housekeeping genes (gapA, infB, mdh, pgi, phoE, rpoB and tonB). A total of 8 different sequence types (STs) were identified;ST11 was dominant sequence type, accounting 41.6 %( 5 isolates) and was harboring combination of TEM-1, SHV-11 and CTX-15 genes.
References
Arlet, G., M.; Rouveau, I.; Casin, P. J.; Bouvet, Lagrange, P. H. and Philippon, A. (1994). Molecular epidemiology of Klebsiella pneumoniae strains that produce SHV-4 beta-lactamase and which were isolated in 14 French hospitals. J. Clin. Microbiol., 32:2553–2558.Brisse, S. D.;Milatovic, A. C.;Fluit, J. V.andSchmitz, F. J.(2000).Epidemiology of quinolone resistance of Klebsiella pneumoniae and Klebsiella oxytocain Europe. Eur. J. Clin. Microbiol. Infect. Dis., 19:64–68.
Damjanova, I.; Toth, A.; Pa´ szti, J.; Hajbel-Ve´ kony, G.; Jakab, M.; Berta, J.; Milch, H. & Fu zi, M. (2008). Expansion and countrywide dissemination of ST11, ST15, and ST147 ciprofloxacin-resistant CTXM-15-type β-lactamase-producing Klebsiella pneumoniae epidemic clones in Hungary in 2005 – the new MRSAs. J AntimicrobChemother., 62, 978–985.
Diancourt, L.; Passet, V.; Verhoef, J.; Grimont, P.A. and Brisse, S. (2005). Multilocus Sequence Typing of Klebsiella pneumoniae Nosocomial Isolates. Journal of clinical microbiology. 43: 4178–4182
Enright, M. C. and Spratt,B. G. (1999).Multilocus sequence typing.Trends Microbiol. 7:482–487.
Feil, E. J.; Li, B. C.;Aanensen, D. M.;Hanage, W. P. andSpratt, B. G. (2004).eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J. Bacteriol.186:1518–1530.
Jolley, K. A.; Chan, M. S. andMaiden, M. C.(2004).mlstdbNet – distributed multi-locus sequence typing (MLST) databases. BMC Bioinformatics 5:86.
Kaur, J.; Chopra, S.; Sheevani, and Mahajan, G. (2013). Double Disc Synergy Test to Detect ESBL Production in Urinary Isolates of Escherichia coli and Klebsiella pneumonia Journal of Clinical and Diagnostic Research, 7: 229-233
Kitchel, BRasheed, J.K.; Patel, J.B.; Srinivasan, A.; Navon-Venezia, S.; Carmeli, Y.; Brolund, A. andGiske, C.G. (2009). Molecular epidemiology of KPC-producing Klebsiella pneumoniae isolates in the United States: clonal expansion of multilocus sequence type 258. Antimicrob.Agents Chemother. 53:3365–3370.
Ko, K.S.; Lee, J.Y.; Baek, J.Y.; Suh, J.Y.; Lee, M.Y.; Choi, J.Y.; Yeom, J.S.; Kim, Y.S.; Jung, S.I.; Shin, S.Y.; Heo, S.T.; Kwon, K.T.; Son, J.S.;, Kim, S.W.; Chang, H.H.; Ki, H.K.; Chung,D.R.; Peck, K.R. and Song, J.H.(2010). Predominance of an ST11 extended-spectrum beta-lactamase-producing Klebsiella pneumoniae clone causing bacteraemia and urinary tract infections in Korea. J Med Microbiol. 59:822-8.
Maiden, M. C.;Bygraves, J. A.;Feil, E.;Morelli, G.; Russell, J. E.,Urwin, R.; Zhang, Q.; Zhou J.;Zurth, K.;Caugant, D. A.; Feavers, I. M.; Achtman, M. and Spratt, B. G. (1998). Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc. Natl. Acad. Sci. USA 95:3140–3145.
Monnet, D. L.; Biddle, J. W.; Edwards, J. R.; Culver, D. H.;Tolson, J. S.;Martone, W. J.;Tenover,F. C. and Gaynes,R. . (1997). Evidence of interhospital transmission of extended-spectrum beta-lactam-resistant Klebsiella pneumoniae in the United States, 1986 to 1993.The National Nosocomial Infections Surveillance. Syst. Infect. Control Hosp. Epidemiol. 18:492–498.
Oteo, J.;Cuevas, O.;López-Rodríguez, I.;Banderas-Florido, A.;Vindel, A.;Pérez-Vázquez, M.;Bautista, V.;Arroyo, M.;García-Caballero, J.;Marín-Casanova, P.;González-Sanz, R.;Fuentes-Gómez, V.; Oña-Compán, S.;García-Cobos, S. andCampos, J.(2009).EmergenceofCTX-M-15-producing Klebsiella pneumoniae of multilocus sequence types 1, 11, 14, 17, 20, 35 and 36 as pathogens and colonizers in newborns and adults.JAntimicrobChemother. 64:524-8.
Paterson, D. L.;Ko, W. C.; Von Gottberg, A.;Mohapatra, S.;Casellas, J. M.;Goossens, H.;Mulazimoglu, L.;Trenholme, G.; Klugman, K. P.; Bonomo, R. A.; Wagener, M.; McCormack, J. G.; andYu, V. L. (2004). International prospective study of Klebsiella pneumoniae bacteremia: implications of extended-spectrum beta-lactamase production in nosocomial infections. Ann. Intern. Med. 140:26–32.
Paterson, D. L.;Mulazimoglu, L.;Casellas, J. M.;Ko, W. C.;Goossens, H.; Von Gottberg,A.;Mohapatra, S.;Trenholme, G. M.;Klugman, K. P.; McCormack, J. G. and Yu, V. L. (2000). Epidemiology of ciprofloxacin resistance and its relationship to extended-spectrum beta-lactamase production in Klebsiella pneumoniae isolates causing bacteremia. Clin. Infect. Dis. 30:473–478.
Qi, Y.; Wei, Z.; Ji, S.; Du, X.; Shen, P.and Yu, Y. (2011). ST11, the dominant clone of KPC-producing Klebsiella pneumoniae in China. J. Antimicrob. Chemother. 66:307–312.
Toth, A.; Damjanova, I.; Puskás, E.; Jánvári, L.; Farkas, M.; Dobák, A.; Böröcz, K. and Pászti J. (2010). Emergence of a colistin-resistant KPC-2-producing Klebsiella pneumoniae ST258 clone in Hungary. Eur. J. Clin. Microbiol.Infect. Dis. 29:765–769.
Yun-Tae, K.; Kim, T.U. and Baik, H. S. (2006). Characterization of Extended Spectrum β-Lactamase Genotype TEM, SHV, and CTX-M producing Klebsiella pneumoniae Isolated from Clinical Specimens in Korea. J. Microbiol., 16:889-895.
(http: //www. blast.ncbi.nlm.nih.gov).
(http://www.pasteur.fr/recherche/genopole/PF8/mlst/Kpneumoniae.html).
(http://www.pasteur.fr/recherche/genopole/PF8/mlst/Kpneumoniae.html)
Damjanova, I.; Toth, A.; Pa´ szti, J.; Hajbel-Ve´ kony, G.; Jakab, M.; Berta, J.; Milch, H. & Fu zi, M. (2008). Expansion and countrywide dissemination of ST11, ST15, and ST147 ciprofloxacin-resistant CTXM-15-type β-lactamase-producing Klebsiella pneumoniae epidemic clones in Hungary in 2005 – the new MRSAs. J AntimicrobChemother., 62, 978–985.
Diancourt, L.; Passet, V.; Verhoef, J.; Grimont, P.A. and Brisse, S. (2005). Multilocus Sequence Typing of Klebsiella pneumoniae Nosocomial Isolates. Journal of clinical microbiology. 43: 4178–4182
Enright, M. C. and Spratt,B. G. (1999).Multilocus sequence typing.Trends Microbiol. 7:482–487.
Feil, E. J.; Li, B. C.;Aanensen, D. M.;Hanage, W. P. andSpratt, B. G. (2004).eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J. Bacteriol.186:1518–1530.
Jolley, K. A.; Chan, M. S. andMaiden, M. C.(2004).mlstdbNet – distributed multi-locus sequence typing (MLST) databases. BMC Bioinformatics 5:86.
Kaur, J.; Chopra, S.; Sheevani, and Mahajan, G. (2013). Double Disc Synergy Test to Detect ESBL Production in Urinary Isolates of Escherichia coli and Klebsiella pneumonia Journal of Clinical and Diagnostic Research, 7: 229-233
Kitchel, BRasheed, J.K.; Patel, J.B.; Srinivasan, A.; Navon-Venezia, S.; Carmeli, Y.; Brolund, A. andGiske, C.G. (2009). Molecular epidemiology of KPC-producing Klebsiella pneumoniae isolates in the United States: clonal expansion of multilocus sequence type 258. Antimicrob.Agents Chemother. 53:3365–3370.
Ko, K.S.; Lee, J.Y.; Baek, J.Y.; Suh, J.Y.; Lee, M.Y.; Choi, J.Y.; Yeom, J.S.; Kim, Y.S.; Jung, S.I.; Shin, S.Y.; Heo, S.T.; Kwon, K.T.; Son, J.S.;, Kim, S.W.; Chang, H.H.; Ki, H.K.; Chung,D.R.; Peck, K.R. and Song, J.H.(2010). Predominance of an ST11 extended-spectrum beta-lactamase-producing Klebsiella pneumoniae clone causing bacteraemia and urinary tract infections in Korea. J Med Microbiol. 59:822-8.
Maiden, M. C.;Bygraves, J. A.;Feil, E.;Morelli, G.; Russell, J. E.,Urwin, R.; Zhang, Q.; Zhou J.;Zurth, K.;Caugant, D. A.; Feavers, I. M.; Achtman, M. and Spratt, B. G. (1998). Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc. Natl. Acad. Sci. USA 95:3140–3145.
Monnet, D. L.; Biddle, J. W.; Edwards, J. R.; Culver, D. H.;Tolson, J. S.;Martone, W. J.;Tenover,F. C. and Gaynes,R. . (1997). Evidence of interhospital transmission of extended-spectrum beta-lactam-resistant Klebsiella pneumoniae in the United States, 1986 to 1993.The National Nosocomial Infections Surveillance. Syst. Infect. Control Hosp. Epidemiol. 18:492–498.
Oteo, J.;Cuevas, O.;López-Rodríguez, I.;Banderas-Florido, A.;Vindel, A.;Pérez-Vázquez, M.;Bautista, V.;Arroyo, M.;García-Caballero, J.;Marín-Casanova, P.;González-Sanz, R.;Fuentes-Gómez, V.; Oña-Compán, S.;García-Cobos, S. andCampos, J.(2009).EmergenceofCTX-M-15-producing Klebsiella pneumoniae of multilocus sequence types 1, 11, 14, 17, 20, 35 and 36 as pathogens and colonizers in newborns and adults.JAntimicrobChemother. 64:524-8.
Paterson, D. L.;Ko, W. C.; Von Gottberg, A.;Mohapatra, S.;Casellas, J. M.;Goossens, H.;Mulazimoglu, L.;Trenholme, G.; Klugman, K. P.; Bonomo, R. A.; Wagener, M.; McCormack, J. G.; andYu, V. L. (2004). International prospective study of Klebsiella pneumoniae bacteremia: implications of extended-spectrum beta-lactamase production in nosocomial infections. Ann. Intern. Med. 140:26–32.
Paterson, D. L.;Mulazimoglu, L.;Casellas, J. M.;Ko, W. C.;Goossens, H.; Von Gottberg,A.;Mohapatra, S.;Trenholme, G. M.;Klugman, K. P.; McCormack, J. G. and Yu, V. L. (2000). Epidemiology of ciprofloxacin resistance and its relationship to extended-spectrum beta-lactamase production in Klebsiella pneumoniae isolates causing bacteremia. Clin. Infect. Dis. 30:473–478.
Qi, Y.; Wei, Z.; Ji, S.; Du, X.; Shen, P.and Yu, Y. (2011). ST11, the dominant clone of KPC-producing Klebsiella pneumoniae in China. J. Antimicrob. Chemother. 66:307–312.
Toth, A.; Damjanova, I.; Puskás, E.; Jánvári, L.; Farkas, M.; Dobák, A.; Böröcz, K. and Pászti J. (2010). Emergence of a colistin-resistant KPC-2-producing Klebsiella pneumoniae ST258 clone in Hungary. Eur. J. Clin. Microbiol.Infect. Dis. 29:765–769.
Yun-Tae, K.; Kim, T.U. and Baik, H. S. (2006). Characterization of Extended Spectrum β-Lactamase Genotype TEM, SHV, and CTX-M producing Klebsiella pneumoniae Isolated from Clinical Specimens in Korea. J. Microbiol., 16:889-895.
(http: //www. blast.ncbi.nlm.nih.gov).
(http://www.pasteur.fr/recherche/genopole/PF8/mlst/Kpneumoniae.html).
(http://www.pasteur.fr/recherche/genopole/PF8/mlst/Kpneumoniae.html)
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Published
2014-12-30
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Khalid, H. M., Jubrael, J. M., & Yousif, S. Y. (2014). Multilocus Sequence Typing of Klebsiella Pneumoniae Producing Extended Spectrum Β-Lactamases Isolated From Kurdistan Region, Iraq. Science Journal of University of Zakho, 2(2), 293–298. Retrieved from https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/229
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