Molecular Identification and Hemolytic Activity of Candida Species Isolated from Urine of Healthy and Diabetic Women in Kurdistan of Iraq

  • Zinah S. Oufi Dept. of Biology, Faculty of Science, University of Zakho, Kurdistan Region, Iraq
  • Ahmed B. Mohammed Dept. of Biology, Faculty of Science, University of Zakho, Kurdistan Region, Iraq
  • Samir Kh. Abdullah Dept. of Medical Laboratory Technology, Alnoor University College, Nineva, Iraq
Keywords: Hemolysin, Candida spp., Urine, Diabetic Mellitus, Sequencing, ITS

Abstract

A survey was carried out on the incidence of Candida yeasts in urine of healthy (non-diabetic) and diabetic women in Duhok and Zakho cities from February 2019 to April 2019. Out of 620 urine samples examined, 144 samples were found positive for Candida infection (23.2%). There were significant differences in incidence of Candida infection between urine of healthy and diabetic women as well as among age groups. All Candida isolates from positive samples were identified by using Chromogenic Candida Agar medium and confirmed by sequencing of the ITS1 and ITS4 region of rDNA. The identified Candida species were C.albicans, C.glabrata, C.krusei and C.tropicalis. The most common species was C.albicans (34%), followed by C.glabrata (29.9%), then C.tropicalis (13.8%), whereas, C.krusei (=Pichia kudriavzevii) displayed the least incidence (9.7%). All tested strains (n=83) for their hemolytic activity on Sabouraud's dextrose agar amended with sheep blood and glucose showed positive activity. Candida albicans and C.tropicalis displayed the highest hemolytic activity.

Author Biographies

Zinah S. Oufi, Dept. of Biology, Faculty of Science, University of Zakho, Kurdistan Region, Iraq

Dept. of Biology, Faculty of Science, University of Zakho, Kurdistan Region, Iraq - (zinahmyco@gmail.com)

Ahmed B. Mohammed, Dept. of Biology, Faculty of Science, University of Zakho, Kurdistan Region, Iraq

Dept. of Biology, Faculty of Science, University of Zakho, Kurdistan Region, Iraq - (ahmed.mohammed@uoz.edu.krd)

Samir Kh. Abdullah, Dept. of Medical Laboratory Technology, Alnoor University College, Nineva, Iraq

Dept. of Medical Laboratory Technology, Alnoor University College, Nineva, Iraq - (samir.abdullah1947@gmail.com)

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Published
2020-03-30
How to Cite
Oufi, Z., Mohammed, A., & Abdullah, S. (2020). Molecular Identification and Hemolytic Activity of Candida Species Isolated from Urine of Healthy and Diabetic Women in Kurdistan of Iraq. Science Journal of University of Zakho, 8(1), 1-6. https://doi.org/10.25271/sjuoz.2020.8.1.684
Section
Science Journal of University of Zakho