Identification of Candida Spp. Isolated From Urine by Phenotypic Methods and Multiplex Pcr in Duhok, Iraq

  • Ahmed B. Mohammed University of Zakho
  • Jumma H. Ali Central Medical Laboratory
  • Samir K. Abdullah University of Zakho
Keywords: Candida species, Multiplex PCR, Urine, Chromogenic Candida agar, Iraq

Abstract

The present study was aimed to isolate and identify Candida species from urine samples obtained from patients with urinary tract infection from Duhok province. Species identification was based on colony color on chromogenic Candida agar as well as multiplex PCR method. Results revealed the prevalence of non-albicans CandidaC.krusei was the most frequently isolated species with the prevalence of 26.3% followed by C.kefyr 23.68%, C.glabrata (18.42% and C.albicans 15.78%. Multiplex PCR method seemed to be more accurate and reliable for identification of Candida yeast.

Author Biographies

Ahmed B. Mohammed, University of Zakho

Dept. of Biology, Faculty of Science, University of Zakho, Kurdistan Region, Iraq

Jumma H. Ali, Central Medical Laboratory

Central Medical Laboratory, Duhok, Kurdistan Region – Iraq.

Samir K. Abdullah, University of Zakho

Dept. of Biology, Faculty of Science, University of Zakho, Kurdistan Region, Iraq.

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Published
2017-03-30
How to Cite
Mohammed, A., Ali, J., & Abdullah, S. (2017). Identification of Candida Spp. Isolated From Urine by Phenotypic Methods and Multiplex Pcr in Duhok, Iraq. Science Journal of University of Zakho, 5(1), 11-15. https://doi.org/10.25271/2017.5.1.292
Section
Science Journal of University of Zakho