Bacteriological and Molecular Characterization of Diarrheagenic Escherichia Coli Pathotypes From Children in Duhok City, Iraq

  • Haveen K. Hasan Technical Institute, Duhok Polytechnic University, Duhok, Iraq
  • Najim A. Yassin Dept. of Medical Microbiology, College of Medicine, Duhok University, Kurdistan Region - Iraq.
  • Souzan H. Eassa Dept. of Histology and Medical Biology, College of Medicine, Duhok University, Kurdistan Region - Iraq.
Keywords: Diarrheagenic Escherichia coli, Stool, MacConkey-Sorbitol agar, Virulence factors, PCR

Abstract

Background: A variety of diarrheagenic E coli (DEC) are responsible for causing different types of diarrhea in children especially in developing courtiers.

Objectives: This study was primarily aimed to isolate and bacteriological characterizing of E coli from diarrheic infant stool and to investigate their antibiotic resistance patterns and then using molecular identification of DEC pathotypes for better discrimination.

Methods: Total of 400 fresh stools specimens were collected from children with diarrhea in Heevi Hospital in Duhok city, Iraq. The samples were cultured on selective media such as (MacConkey and MacConkey sorbitol agar). Colonies were identified through biochemical reaction and VITEK 2 system and then antibiotic susceptibility profiles were determined.

Results: A total of 349(87.2%) samples were yielded positive for growth of E coli. Out of these, 50 phenotypically-identified E coli were then subjected to PCR assay targeting certain virulence factors (alt, eae, sxt1 and sxt2) for discrimination of pathotyes. 13/50(26%) Enterotoxigenix E. coli (ETEC) was detected, 5/50(10%) Enterohemorrhagic E coli EHEC was detected, while no Enteropathogenic E coli (EPEC) was detected. All pathotypes were more frequent in samples from male children aged between 2-3 years that were artificial feeding pattern. Moreover, all pathotypes expressed high resistant to ampicillin, cephalosporin and tetracycline while little resistance to imipenem was observed. Conclusion: The study concludes presence of diarrheagenic E coli pathotypes in our community causing diarrhea in children and emphasize on using of PCR assay for best discrimination. 

Author Biographies

Haveen K. Hasan, Technical Institute, Duhok Polytechnic University, Duhok, Iraq

Technical Institute, Duhok Polytechnic University, Duhok, Iraq (haveenbio@gmail.com).

Najim A. Yassin, Dept. of Medical Microbiology, College of Medicine, Duhok University, Kurdistan Region - Iraq.

Dept. of Medical Microbiology, College of Medicine, Duhok University, Kurdistan Region - Iraq (najim56@yahoo.com).

Souzan H. Eassa, Dept. of Histology and Medical Biology, College of Medicine, Duhok University, Kurdistan Region - Iraq.

Dept. of Histology and Medical Biology, College of Medicine, Duhok University, Kurdistan Region - Iraq (souzan.eassa@uod.ac)

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Published
2020-06-30
How to Cite
Hasan, H., Yassin, N., & Eassa, S. (2020). Bacteriological and Molecular Characterization of Diarrheagenic Escherichia Coli Pathotypes From Children in Duhok City, Iraq. Science Journal of University of Zakho, 8(2), 52-57. https://doi.org/10.25271/sjuoz.2020.8.1.702
Section
Science Journal of University of Zakho