Comparison of Mat with Elisa and Lat Tests in Detecting Toxoplasma Gondii Antibodies in Human Sera

Authors

  • Hemdad H. Mawlood University of Zakho
  • Wijdan M. S. Mero University of Zakho
  • Chunlei Su University of Tennessee
  • Abdallah M. Isa Tennessee State University

DOI:

https://doi.org/10.25271/2017.5.1.296

Keywords:

Toxoplasma gondii, MAT, LAT, ELISA

Abstract

The modified agglutination test (MAT) has been widely used for the detection of Toxoplasma gondii infection in numerous animal species. For the standard protocol of MAT test, T. gondii whole cell antigens were produced in the laboratory mice, which was a tedious process. The produced antigen in cell culture was used to assure its capability for MAT test.  For detecting the antibodies of T. gondii in human sera, comparison was made between MAT, enzyme linked immunosorbent assay (ELISA) and latex agglutination test (LAT).  A total of 96 human serum samples were tested. The anti-Toxoplasma IgG antibodies were found in 25.0% (24/96), 20.8% (20/96) and 13.5% (13/96) samples by MAT (cutoff 1:25), ELISA and LAT tests, respectively.  The MAT and ELISA tests matched 95.8% in detection of IgG antibodies, with the positive percent agreement of 100% (20/20) and negative percent agreement of 94.7% (72/76).  For the MAT versus LAT tests, the overall agreement was 88.5% (85/96), with the positive percent agreement of 100% (13/13) and negative percent agreement of 86.7% (72/83). These results suggest a strong correlation between the MAT and ELISA tests in detecting serum IgG to T. gondii in human sera.  In conclusion, T. gondii prepared in cell culture provides an alternative solution to produce antigens for MAT test. 

Author Biographies

Hemdad H. Mawlood, University of Zakho

Dept. of Biology, Faculty of Science, University of Zakho, Kurdistan Region, Iraq.

Wijdan M. S. Mero, University of Zakho

Dept. of Biology, Faculty of Science, University of Zakho, Kurdistan Region, Iraq.

Chunlei Su, University of Tennessee

Dept. of Microbiology, the University of Tennessee, Knoxville, TN. USA.nl

Abdallah M. Isa, Tennessee State University

Dept. of Biological Science, School of Life and Physical Sciences, Tennessee State University, USA.

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Published

2017-03-30

How to Cite

Mawlood, H. H., Mero, W. M. S., Su, C., & Isa, A. M. (2017). Comparison of Mat with Elisa and Lat Tests in Detecting Toxoplasma Gondii Antibodies in Human Sera. Science Journal of University of Zakho, 5(1), 28–31. https://doi.org/10.25271/2017.5.1.296

Issue

Vol. 5 No. 1 (2017): March, 2017

Section

Science Journal of University of Zakho

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